Antibody-Free Colorimetric Detection of Total Aflatoxins in Rice Based on a Simple Two-Step Chromogenic Reaction

文献类型： 外文期刊

第一作者： Du, Bibai

作者： Du, Bibai;Yang, Kunhao;Pan, Long;Liu, Qingju;Gong, Lingling;Yang, Jingkui;He, Yujian;Su, Xiaoou;Wang, Peilong;He, Yujian

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期刊名称：ANALYTICAL CHEMISTRY （ 影响因子：6.986； 五年影响因子：6.755 ）

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收录情况： SCI

摘要： The prevalently used immunoassays for fast screening, of aftatoxins (AFs) usually cannot meet the requirement for simultaneous determination of total AFs (aflatoxin B-1 + aflatoxin B-2 + aflatoxin G(1) + aflatoxin G(2)) due to the deficiency of highly group-specific antibodies. This paper describes a two-step chromogenic reaction based method to quantitatively detect total AFs in rice using colorimetric measurement without antibody. In the method, colorless AFs transform into green colored indophenol products through the reaction with sodium hydroxide and 2,6-dibromoquinone-4-chloroimide (DBQC) successively, allowing selectively determining, total AFs up to 3.9 mu g/kg over other competitive mycotoxins under optimal conditions by a UV-vis spectrophotometer. In addition, the colorimetric measurement results of the rice samples agree well with that of a standard HPLC method, demonstrating the good reliability and applicability of the method. Uniquely, the Method has potential for on-site detection of total AFs in rice when using a nylon membtane-based device.

分类号： O65

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