Expression Profile of Antioxidant Enzymes in Hemocytes from Freshwater Prawn Macrobrachium rosenbergii Exposed to an Elevated Level of Copper
文献类型: 外文期刊
第一作者: Guo, Hui
作者: Guo, Hui;Miao, Yu-Tao;Qian, Kun;Wang, An-Li;Guo, Hui;Xian, Jian-An
作者机构:
关键词: Macrobrachium rosenbergii;Copper;Antioxidant enzyme;Gene expression;Hemocyte
期刊名称:BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY ( 影响因子:2.151; 五年影响因子:2.129 )
ISSN:
年卷期:
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收录情况: SCI
摘要: This study evaluated the expression level of antioxidant enzymes Cu, Zn-superoxide dismutase (Cu, Zn-SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione S-transferase (GST) in hemocytes of Macrobrachium rosenbergii exposed to copper by real-time PCR (qRT-PCR). Results showed that the mRNA expression of Cu, Zn-SOD increased to reach a peak at 6 h, then recovered to its normal level at 48 h. CAT expression level was significantly increased at 12 h and reached a peak at 24 h, but recovered to its normal level later. GPx expression level was significantly increased at 6 h and reached the peak at 12 h. GST expression level was significantly induced from 12 to 24 h and then dropped to its normal level at 48 h. These results indicated that antioxidant enzymes were inducible, possibly for removing excessive reactive oxygen species to protect prawn from oxidative stress.
分类号: R99
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