Discovery of genes associated with cadmium accumulation from gill of scallop Chlamys farreri based on high-throughput sequencing
文献类型: 外文期刊
第一作者: Zhang, Hui
作者: Zhang, Hui;Zhai, Yuxiu;Yao, Lin;Jiang, Yanhua;Li, Fengling;Zhang, Hui;Zhai, Yuxiu;Yao, Lin;Jiang, Yanhua;Li, Fengling;Zhang, Hui;Zhai, Yuxiu;Yao, Lin;Jiang, Yanhua;Li, Fengling
作者机构:
关键词: Comparative transcriptomics;Chlamys farreri;Cadmium accumulation;Differentially expressed genes
期刊名称:GENES & GENOMICS ( 影响因子:1.839; 五年影响因子:1.329 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: Chlamys farreri is an important economic mollusk and is able to accumulate cadmium (Cd) excessively. Two cDNA libraries from gill of C. farreri which were Cd treated or not were generated based on the Illumina sequencing platform in order to identify differentially expressed genes (DEGs) associated with cadmium accumulation. The analysis of comparative transcriptomics identified 82,800 unigenes and 128 differentially expressed genes. Of these, 8 of the most significant DEGs were verified by real-time PCR, showing consistent trends in expression patterns between the two techniques. KEGG pathways analysis revealed that 37 associated processes were highly enriched. A set of key DEGs were identified that may play important roles in cadmium exposure. Our work presents an overview of gene expression change during cadmium exposure to C. farreri and identifies a series of candidate genes and pathways for further research on the molecular mechanisms of cadmium accumulation.
分类号: Q
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