New Targets and Cofactors for the Transcription Factor LrpA from Mycobacterium tuberculosis

文献类型： 外文期刊

第一作者： Song, Ningning

作者： Song, Ningning;Cui, Yingying;Li, Zhaoli;Chen, Liping;Liu, Siguo;Cui, Yingying

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期刊名称：DNA AND CELL BIOLOGY （ 影响因子：3.311； 五年影响因子：3.557 ）

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收录情况： SCI

摘要： Rv3291c (MtbLrpA), a transcriptional regulator, belongs to the leucine-responsive regulatory protein (Lrp) family and is thought to play an important role in Mycobacterium tuberculosis persistence. In this study, we verified 17 novel potential binding sites for MtbLrpA by in vitro binding assays on the basis of previous predictions from an in silico analysis and bacterial one-hybrid (BIH) reporter system. Amino acids, such as tyrosine, phenylalanine, tryptophan, and histidine, strongly affect the binding affinity of MtbLrpA, and vitamins, including B-1, B-3, B-6, V-C, B-7, B-9, B-12, V-A, and V-K3, also decrease MtbLrpA binding affinity. This is the first report regarding that an Lrp-like protein can sense vitamins as an environmental signal. Vitamin supplementation to the environment can change the expression level of the target genes, which provides a potential mechanism for tuberculosis supplementary treatment with vitamins.

分类号： Q343

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