Rapid and highly efficient callus induction and plant regeneration in the starch-rich duckweed strains of Landoltia punctata

文献类型： 外文期刊

第一作者： Huang, Meng

作者： Huang, Meng;Fu, Lili;Sun, Xuepiao;Zhang, Jiaming;Di, Rong

作者机构：

关键词： Tissue culture;Explant orientation;Lemnaceae;Callus induction;Frond regeneration

期刊名称：ACTA PHYSIOLOGIAE PLANTARUM （ 影响因子：2.354； 五年影响因子：2.711 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： The starch-rich duckweed Landoltia punctata is a valuable aquatic plant in wastewater purification, bioenergy production, and many other applications. A highly efficient callus induction and plant regeneration protocol is desirable so that biotechnology can be used to develop new varieties with added value and adaptation. We studied both known and unknown factors that influence callus induction in L. punctata and obtained almost 100 % induction rate in 30 days. The optimum medium for callus induction was MS basal medium supplemented with 1 % sorbitol, 15 mg/L 2,4-D, and 2 mg/L 6-BA. Green fragile callus was induced from the meristematic region in the budding pouches. The optimum photoperiod for callus induction was 16-h day, and the optimum explant orientation was dorsal side down on the medium. The optimum medium for callus subculture was WPM basal medium supplemented with 2 % sorbitol, 4 mg/L 2,4-D, and 0.5 mg/L TDZ. Green callus could be maintained by subculture once every 4 weeks. However, when the subculture cycle was prolonged to 6 weeks or longer, yellow fragile embryogenic callus was obtained. The optimum plant regeneration medium was MS medium supplemented with 0.5 % sucrose, 1 % sorbitol, and 1.0 mg/L 6-BA with frond regeneration rates of approximately 90 %. The regenerated fronds rooted in Hoagland's liquid medium in 1 week. The callus induction and frond regeneration protocol was tested for its efficiency in geographically distinct strains 5502, 8721, and 9264. Thus, we obtained a rapid and efficient protocol for callus induction and frond regeneration of L. punctata, which takes only 9 weeks.

分类号： Q94

  • 相关文献

[1]Species distribution, genetic diversity and barcoding in the duckweed family (Lemnaceae). Xu, Yaliang,Ma, Shuai,Huang, Meng,Peng, Ming,Zhang, Jiaming,Xu, Yaliang,Bog, Manuela,Appenroth, Klaus-J.,Sree, K. Sowjanya.

[2]Adventitious shoot regeneration from leaves of apple rootstock 'Pingyitiancha' (Malus hupehensis var. pinyiensis) and genetic fidelity of regenerated plantlets using SSR markers. Jin, Wanmei,Wang, Yuanhua,Wang, Hua. 2014

[3]Efficient somatic embryogenesis in sugar beet (Beta vulgaris L.) breeding lines. Zhang, Chun-Lai,Chen, Dong-Fang,Kubalakova, Marie,Zhang, Jian,Scott, Nigel W.,Elliott, Malcolm C.,Slater, Adrian,Zhang, Chun-Lai,Chen, Dong-Fang,Kubalakova, Marie,Zhang, Jian.

[4]High Efficiency Shoot Regeneration from Leaf Explants of Taishan Sour Jujube (Zizyphus spinosus Hu.). Sun, H.,Sun, Q.,Zhou, G.,Liu, Q.,Xin, L.. 2009

[5]High-Efficiency Plant Regeneration from Immature Inflorescence Derived Callus Cultures of Two Phenotypically Distinct Accessions of Centipedegrass (Eremochloa ophiuroides). Ma, Jingjing,Wang, Yi,Zong, Junqin,Zhang, Bing,Chen, Jingbo,Li, Dandan,Li, Ling,Guo, Hailin,Liu, Jianxiu,Li, Jianjian,She, Jianming. 2018

[6]Comparison of dynamic changes of endogenous hormones between calli derived from mature and immature embryos of maize. Wang, Henning,Zhang, Jinwen,Jia, Xiaoxia,Liang, Huiguang,Kong, Weiping,Zhang, Xinhui. 2011

[7]Establishment of a highly efficient regeneration method from the scraped broken embryo of mature barley seed. Hua, Wei,Zhu, Jinghuan,Shang, Yi,Wang, Junmei,Jia, Qiaojun,Lin, Feng,Yang, Jianming. 2013

[8]Shoot organogenesis and plant regeneration in Agave hybrid, No. 11648. Zhang, Yan-Mei,Li, Jun-Feng,Lu, Jun-Ying,Zhou, Wen-Zhao,Li, Xin,Chen, Zhi. 2013

[9]Production of triploid plants of papaya by endosperm culture. Sun, De-Quan,Lu, Xin-Hua,Mo, Yi-Wei,Xie, Jiang-Hui,Liang, Guo-Lu,Guo, Qi-Gao.

[10]Callus induction and plant regeneration in Alternanthera philoxeroides. Gao, Jianming,Li, Jinzhi,Luo, Caibo,Yin, Liang,Li, Shengchun,Yang, Guangxiao,He, Guangyuan,Gao, Jianming,Li, Jinzhi.

[11]Low temperature treatments of rice (Oryza sativa L.) anthers changes polysaccharide and protein composition of the anther walls and increases pollen fertility and callus induction. Tian, Q. Q.,Li, X.,Fang, X. W.,Tian, Q. Q.,Lu, C. M.,Li, X..

[12]Limited tissue culture-induced mutations and linked epigenetic modifications in F-1 hybrids of sorghum pure lines are accompanied by increased transcription of DNA methyltransferases and 5-methylcytosine glycosylases. von Wettstein, Diter,Zhang, Meishan,Xu, Chunming,Zhao, Na,Liu, Bao,Zhang, Meishan,Yan, Hongyan. 2009

[13]Molecular cytogenetic identification of Triticum aestivum Secale cereale substitution and addition lines. Li, HJ,Zhu, ZQ,Zhang, YM,Guo, BH,Wen, YX,Jia, X. 1998

[14]Bioaccumulation of microcystin and its oxidative stress in the apple (Malus pumila). Chen, Jianzhong,Zhang, Haiyang,Wang, Chenyi,Zhou, Guoqing,Han, Zhiping,Chen, Jianzhong,Liu, Zhili,Dai, Jian. 2010

[15]Variation of B Chromosome Associated with Tissue Culture in Wheat-rye Cross. Li, Hongjie,Tian, Bohong. 2009

[16]Comparison of three ELISA methods for the detection of apple chlorotic leaf spot virus and apple stem grooving virus. Wu, YQ,Zhang, DM,Chen, SY,Wang, XF,Wang, WH. 1998

[17]Molecular cytogenetic analysis of intergeneric chromosomal translocations between wheat (Triticum aestivum L.) and Dasypyrum villosum arising from tissue culture. Li, HJ,Guo, BH,Li, YW,Du, LQ,Jia, X,Chu, CC. 2000

[18]The Research on Actinidia eriantha Benth. in South China. Wu, Y. J.,Xie, M.,Zhang, Q. C.,Zhang, H. Q.,Fang, J. B.. 2011

[19]Investigation of metabolites accumulation in medical plant Gentiana rigescens during different growing stage using LC-MS/MS and FT-IR. Pan, Yu,Zhang, Ji,Zhao, Yan-Li,Wang, Yuan-Zhong,Pan, Yu,Huang, Heng-Yu. 2015

[20]Dramatic genotypic difference in, and effect of genetic crossing on, tissue culture-induced mobility of retrotransposon Tos17 in rice. Lin, Chunjing,Hu, Lanjuan,Yang, Jingjing,Zhou, Tianqi,Xu, Chunming,Liu, Bao,Lin, Chunjing,Lin, Xiuyun,Long, Likun,Xing, Shaochen,Dong, Yingshan,Qi, Bao. 2012

作者其他论文 更多>>

微信小程序