The genes involved in the protective effects of phytohormones in response to Verticillium dahliae infection in Gossypium hirsutum

文献类型： 外文期刊

第一作者： Zhang, Wenwei

作者： Zhang, Wenwei;Jian, Guiliang;Qi, Fangjun;Si, Ning

作者机构：

关键词： Gossypium hirsutum;Phytohormone signal pathways;Quantitative real-time PCR;Verticillium dahliae;Virus-induced gene silencing

期刊名称：JOURNAL OF PLANT BIOLOGY （ 影响因子：2.434； 五年影响因子：2.455 ）

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收录情况： SCI

摘要： Verticillium wilt disease caused by soil-borne fungus Verticillium dahliae (V. dahliae), is a serious threats to cotton growth worldwide. Endogenous phytohormones, such as salicylic acid (SA), jasmonic acid (JA), ethylene (ET), and brassinosteroid (BR) have been reported to play important roles in defense response in plants. Based on highthroughput RNA sequencing analysis of resistant Gossypium hirsutum (G. hirsutum) variety Zhongzhimian KV-3, comparing the transcriptome with V. dahliae infection and non-infection, eight differentially expressed candidate genes functions indicated in SA (GhPUB17, GhTGA7 and GhPR1), JA (GhJAZ10 and GhbHLH18), ET (GhEBF1), cytokinine (GhE13L13) and BR (GhBZR1) signal pathways were investigated via virus-induced gene silencing (VIGS) using cotton leaf crumple virus (CLCrV) vector. The expression of eight genes was further validated through quantitative realtime PCR. The result showed that the expression level of GhPUB17, GhJAZ10, GhbHLH18, GhEBF1 and GhE13L13 increased faster in resistant variety Zhongzhimian KV-3 than in susceptible variety 86-1 upon V. dahliae infection. Silencing up-regulated genes GhPUB17, GhJAZ10, GhbHLH18 and GhEBF1 significantly enhanced resistant variety susceptibility to V. dahliae and silencing down-regulated genes GhTGA7 and GhBZR1 significantly increased susceptible variety resistance to V. dahliae. Taken together, these genes are important components in response to V. dahliae infection.

分类号： Q94

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