Effect of the glycosyltransferases on the capsular polysaccharide synthesis of Streptococcus suis serotype 2

文献类型： 外文期刊

第一作者： Zhang, Yanyan

作者： Zhang, Yanyan;Ding, Dandan;Yang, Xiaopei;Zong, Bingbing;Wang, Xiangru;Chen, Huanchun;Bei, Weicheng;Tan, Chen;Liu, Manli;Zhang, Yanyan;Ding, Dandan;Yang, Xiaopei;Zong, Bingbing;Wang, Xiangru;Chen, Huanchun;Bei, Weicheng;Tan, Chen;Zhang, Yanyan;Ding, Dandan;Yang, Xiaopei;Zong, Bingbing;Wang, Xiangru;Chen, Huanchun;Bei, Weicheng;Tan, Chen

作者机构：

关键词： Streptococcus suis serotype 2;S. suis 2 SC19;Capsular polysaccharide;cps2E/cps2G/cps2J/cps2L;Virulence

期刊名称：MICROBIOLOGICAL RESEARCH （ 影响因子：5.415； 五年影响因子：6.038 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： Streptococcus suis serotype 2 (S. suis 2) is a serious zoonotic pathogen causing septicemia and meningitis in piglets and humans. The capsular polysaccharide (CPS) is an essential virulence factor for S. suis 2 to infect the host. The synthesis of CPS repeating units involves multiple glycosyltransferases. In this study, four genes (cps2E, cps2G, cps2J and cps2L) encoding different glycosyltransferases involved in CPS synthesis were researched in S. suis 2. Four deletion mutants (Delta cps2E, Delta cps2G, Delta cps2J and Delta cps2L) with their CPS incomplete and their sialic acid content significantly decreased were constructed in S. suis 2 SC19. All these four mutant strains showed enhanced adhesion to Hep-2 cells and increased sensitivity to phagocytosis. Flow cytometric analysis also revealed that these four mutants were more susceptible to the attack by the complement system. In a mouse model of infection, the mutant strains were rapidly cleared by the immune system, compared with the wild-type strain. In summary, this study characterized four genes (cps2E, cps2G, cps2J and cps2L) involved in CPS synthesis of S. suis 2 SC19 and it revealed that these genes were all crucial for SC19 to invade and survive in the host. (C) 2016 Elsevier GmbH. All rights reserved.

分类号： Q

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