Self-priming on the plant viral RNAs during reverse transcription-PCR

文献类型： 外文期刊

第一作者： Zhang, C.

作者： Zhang, C.;Wu, H. N.;Zhang, Y. Q.;Li, W. M.;Shen, J. G.

作者机构：

关键词： RT-PCR;primer-independent cDNA synthesis;self-priming;plant RNA virus;RT temperature

期刊名称：ACTA VIROLOGICA （ 影响因子：1.162； 五年影响因子：1.255 ）

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年卷期：

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收录情况： SCI

摘要： The occurrence of the primer-independent cDNA synthesis during RT-PCR analysis of human and animal RNA viruses has been well documented. Conversely, there is scant knowledge about this event in plant RNA viruses. Here we show that the primer-independent cDNA synthesis occurs in all eight different plant RNA viruses tested in this study, suggesting a common phenomenon for RT-PCR analysis of plant RNA viruses. Additional experiments indicate that the event is likely contributed to by RNA self-priming, and can be effectively reduced or eliminated through increasing temperature of the RT reaction.

分类号： R37

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