Phloretin promotes adipocyte differentiation in vitro and improves glucose homeostasis in vivo

文献类型： 外文期刊

第一作者： Shu, Gang

作者： Shu, Gang;Lu, Nai-Sheng;Zhu, Xiao-Tong;Du, Min-Qing;Xie, Qiu-Ping;Zhu, Can-Jun;Xu, Qi;Wang, Song-Bo;Wang, Li-Na;Gao, Ping;Xi, Qian-Yun;Zhang, Yong-Liang;Jiang, Qing-Yan;Lu, Nai-Sheng;Xu, Yong;Jiang, Qing-Yan

作者机构：

关键词： Phloretin;FAT/CD36;Preadipocytes;Differentiation;Akt signaling;Glucose

期刊名称：JOURNAL OF NUTRITIONAL BIOCHEMISTRY （ 影响因子：6.048； 五年影响因子：6.114 ）

ISSN：

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页码：

收录情况： SCI

摘要： Adipocyte dysfunction is associated with many metabolic diseases such as obesity, insulin resistance and diabetes. Previous studies found that phloretin promotes 3T3-L1 cells differentiation, but the underlying mechanisms for phloretin's effects on adipogenesis remain unclear. In this study, we demonstrated that phloretin enhanced the lipid accumulation in porcine primary adipocytes in a time-dependent manner. Furthermore, phloretin increased the utilization of glucose and nonesterified fatty acid, while it decreased the lactate output. Microarray analysis revealed that genes associated with peroxisome proliferator-activated receptor-gamma (PPAR gamma), mitogen-activated protein kinase and insulin signaling pathways were altered in response to phloretin. We further confirmed that phloretin enhanced expression of PPAR gamma, CAAT enhancer binding protein-alpha (C/EBP alpha) and adipose-related genes, such as fatty acids translocase and fatty acid synthase. In addition, phloretin activated the Akt (Thr308) and extracellular signal-regulated kinase, and therefore, inactivated Akt targets protein. Wortmannin effectively blocked the effect of phloretin on Akt activity and the protein levels of PPAR gamma, C/EBP alpha and fatty acid binding protein-4 (FABP4/aP2). Oral administration of 5 or 10 mg/kg phloretin to C57BL BKS-DB mice significantly decreased the serum glucose level and improved glucose tolerance. In conclusion, phloretin promotes the adipogenesis of porcine primary preadipocytes through Akt-associated signaling pathway. These findings suggested that phloretin might be able to increase insulin sensitivity and alleviate the metabolic diseases. (C) 2014 Published by Elsevier Inc.

分类号： Q5

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