The effects of Mycoplasma hyopneumoniae on porcine circovirus type 2 replication in vitro PK-15 cells
文献类型: 外文期刊
第一作者: Wang, Haiyan
作者: Wang, Haiyan;Feng, Zhixin;Wu, Yuzi;Wei, Yanna;Gan, Yuan;Hua, Lizhong;Li, Bin;Wang, Xiaomin;Liu, Maojun;Xiong, Qiyan;Shao, Guoqing;Wang, Haiyan;Feng, Zhixin;Shao, Guoqing
作者机构:
关键词: Porcine circovirus type 2;Mycoplasma hyopneumoniae;Co-infection;Replication
期刊名称:RESEARCH IN VETERINARY SCIENCE ( 影响因子:2.534; 五年影响因子:2.382 )
ISSN:
年卷期:
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收录情况: SCI
摘要: Porcine circovirus type 2 (PCV2) is the causative agent of postweaning multisystemic wasting syndrome (PMWS). Mycoplasma hyopneumoniae (Mhp) is a very well-known co-factor that potentially enhances PCV2 replication and thus the development of PMWS. However, co-infection with Mhp and PCV2 in vivo under different conditions can produce divergent clinical signs and lesions. In this study, PCV2 replication could be enhanced by subsequent co-inoculation with Mhp (PCV2 + Mhp) in a time and dose dependent method, but not by prior (Mhp + PCV2) or simultaneous (Mhp/PCV2) co-inoculation. Furthermore, different magnitudes of PCV2-infected cells, varying from 150% +/- 14% to 351% +/- 28%, were detected when co-infected with different Mhp strains. The relative percentage of PCV2-infected cells greatly decreased from 351%+/- 28 to 141%+/- 8 when the Mhp strain was treated with UV light for 12 h. These results offer the evidences to better understand the complex clinical syndromes in Mhp/PCV2 co-infection cases, and the occurrence of PMWS. (C) 2016 Published by Elsevier Ltd.
分类号: S85
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