Complete nucleotide sequence of little cherry virus 1 (LChV-1) infecting sweet cherry in China

文献类型: 外文期刊

第一作者: Wang, Jiawei

作者: Wang, Jiawei;Zhu, Dongzi;Tan, Yue;Zong, Xiaojuan;Wei, Hairong;Liu, Qingzhong;Hammond, Rosemarie W.

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期刊名称:ARCHIVES OF VIROLOGY ( 影响因子:2.574; 五年影响因子:2.466 )

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收录情况: SCI

摘要: Little cherry virus 1 (LChV-1), associated with little cherry disease (LCD), has a significant impact on fruit quality of infected sweet cherry trees. We report the full genome sequence of an isolate of LChV-1 from Taian, China (LChV-1-TA), detected by small-RNA deep sequencing and amplified by overlapping RT-PCR. The LChV-1-TA genome was 16,932 nt in length and contained nine open reading frames (ORFs), with sequence identity at the overall genome level of 76 %, 76 %, and 78 % to LChV-1 isolates Y10237 (UW2 isolate), EU715989 (ITMAR isolate) and JX669615 (V2356 isolate), respectively. Based on the phylogenetic analysis of HSP70h amino acid sequences of Closteroviridae family members, LChV-1-TA was grouped into a well-supported cluster with the members of the genus Velarivirus and was also closely related to other LChV-1 isolates. This is the first report of the complete nucleotide sequence of LChV-1 infecting sweet cherry in China.

分类号: R37

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