MicroRNA Bta-miR-181a regulates the biosynthesis of bovine milk fat by targeting ACSL1

文献类型： 外文期刊

第一作者： Lian, S.

作者： Lian, S.;Nan, X. M.;Ma, L.;Bu, D. P.;Lian, S.;Guo, J. R.;Loor, J. J.;Loor, J. J.;Bu, D. P.;Bu, D. P.

作者机构：

关键词： bta-miR-181a;ACSL1;bovine mammary epithelial cell

期刊名称：JOURNAL OF DAIRY SCIENCE （ 影响因子：4.034； 五年影响因子：4.354 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： MicroRNA (miRNA) are a class of small noncoding RNA that function as important posttranscriptional regulators of gene expression. The acyl-CoA synthetase long-chain family member 1 (ACSL1) is an important enzyme in the process of milk lipid synthesis. In a previous study dealing with incubations of stearic acid in bovine mammary epithelial cells, an opposite expression pattern was observed between ACSL1 and miR-181a. Bioinformatics analysis with TargetScan and PicTar revealed ACSL1 as a potential target gene of miR-181a. The objective of this work was to determine the potential function of miR-181a on milk fat synthesis by defining the regulatory relationship between miR-181a and ACSL1. Primary bovine mammary epithelial cells were harvested from mid-lactation cows and cultured in Dulbecco's modified Eagle's medium/F-12 medium with 10% fetal bovine serum, 0.5 mu g/mL of insulin, 10 ng/mL of epidermal growth factor, 5 mu g/mL of transferrin, 1 mu g/mL of hydrocortisone, 1 mu g/mL of progesterone, 5 mu g/mL of estradiol, and 5 mu g/mL of prolactin. Cells were transfected with an miR-181a mimic to increase its expression and an miR-181a inhibitor to decrease its expression before culturing for 48 h. The results revealed that the overexpression of miR-181a inhibited the expression of ACSL1, whereas the downregulation of miR-181a increased ACSL1 expression. Western blot analysis of ACSL1 revealed similar effects. Oil-red-O staining indicated that cellular lipid droplet synthesis was decreased with the overexpression of bta-miR-181a, and treatment with the bta-miR-181a inhibitor increased concentration of lipid droplets. Furthermore, overexpression of bta-miR-181a resulted in a decrease in concentration of triacylglycerol in the cells, whereas inhibition of bta-miR-181a increased concentration of triacylglycerol. Therefore, the results indicated that bta-miR-181a may contribute to negative regulation of lipid synthesis in mammary cells via targeting ACSL1.

分类号： TS252

  • 相关文献

[1]Characterization of a long-chain fatty acid-CoA ligase 1 gene and association between its SNPs and growth traits in the clam Meretrix meretrix. Dai, Ping,Huan, Pin,Wang, Hongxia,Liu, Baozhong,Dai, Ping,Lu, Xia.

[2]Expression and genome polymorphism of ACSL1 gene in different pig breeds. Li, Qinggang,Tao, Zhu,Shi, Lihua,Ban, Dongmei,Zhang, Bo,Yang, Yuzeng,Zhang, Hao,Wu, Changxin,Li, Qinggang.

[3]Immortalized bovine mammary epithelial cells express stem cell markers and differentiate in vitro. Hu, Han,Zheng, Nan,Hu, Han,Gao, Haina,Dai, Wenting,Zhang, Yangdong,Li, Songli,Wang, Jiaqi.

[4]Heat-induced apoptosis and gene expression in bovine mammary epithelial cells. Hu, Han,Wang, Jiaqi,Gao, Haina,Li, Songli,Zhang, Yangdong,Zheng, Nan,Hu, Han,Wang, Jiaqi,Gao, Haina,Li, Songli,Zhang, Yangdong,Zheng, Nan,Hu, Han,Wang, Jiaqi,Gao, Haina,Li, Songli,Zhang, Yangdong,Zheng, Nan.

[5]Identification and characterization of microRNA sequences from bovine mammary epithelial cells. Bu, D. P.,Nan, X. M.,Wang, F.,Wang, J. Q.,Bu, D. P.,Wang, F.,Bu, D. P.,Nan, X. M.,Loor, J. J.,Loor, J. J..

作者其他论文 更多>>

微信小程序