Target-site basis for resistance to imazethapyr in redroot amaranth (Amaranthus retroflexus L.)

文献类型： 外文期刊

第一作者： Huang, Zhaofeng

作者： Huang, Zhaofeng;Chen, Jinyi;Zhang, Chaoxian;Huang, Hongjuan;Wei, Shouhui;Chen, Jingchao;Wang, Xu;Zhou, Xinxin

作者机构：

关键词： Acetolactate synthase (ALS);Herbicide resistance;Imazethapyr;Arabidopsis

期刊名称：PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：3.963； 五年影响因子：4.454 ）

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收录情况： SCI

摘要： Experiments were conducted to confirm imazethapyr resistance in redroot amaranth (Amaranthus retroflexus L.) and study the target-site based mechanism for the resistance. Whole-plant response experiments revealed that the resistant (R) population exhibited 19.16 fold resistance to imazethapyr compared with the susceptible (S) population. In vitro ALS activity assay demonstrated that the imazethapyr I-50 value of the R population was 21.33 times greater than that of the S population. However, qRT-PCR analysis revealed that there is no difference in ALS gene expression between the R and S populations. Sequence analysis revealed an Asp-376-Glu substitution in ALS in the R population. In order to verify that the imazethapyr resistance was conferred by Asp-376-Glu mutation, the ALS-R and ALS-S genes were fused to the CaMV 35S promoter and introduced into Arabidopsis respectively. The expression of ALS-R in transgenic Arabidopsis plants exhibited 13.79 fold resistance to imazethapyr compared to ALS-S transgenic Arabidopsis. (C) 2015 Elsevier Inc. All rights reserved.

分类号： S4

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