Molecular mechanisms of novel peptides from silkworm pupae that inhibit alpha-glucosidase

文献类型: 外文期刊

第一作者: Zhang, Yu

作者: Zhang, Yu;Wang, Wei;Wang, Junhong;Zhu, Zuoyi;Lia, Xue;Wang, Nan;Wang, Wei

作者机构:

关键词: Molecular mechanism;alpha-glucosidase;In-silico screening;Silkworm pupae

期刊名称:PEPTIDES ( 影响因子:3.75; 五年影响因子:3.389 )

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收录情况: SCI

摘要: The objectives of this study were to identify peptides that inhibit alpha-glucosidase using a quantitative structure-activity relationship (QSAR) screening method and a database of silkworm peptides. This study compared the docking characteristics of several peptides with high inhibitory activity against alpha-glucosidase and summarized the molecular mechanisms by which the silkworm peptides affected alpha-glucosidase. Four peptides that strongly inhibited alpha-glucosidase were obtained: Gln-Pro-Gly-Arg with IC50 at 65.8 mu mol/L, Ser-Gln-Ser-Pro-Ala at 20 mu mol/L, Gln-Pro-Pro-Thr at 560 mu mol/L and Asn-Ser-Pro-Arg at 205 mu mol/L. Studies docking the peptides to the active site of alpha-glucosidase (PDB ID: 2QMJ) showed that a common characteristic was Lys776 in 2QMJ, which could be a critical target for alpha-glucosidase trapping of inhibitory peptides. The results revealed that the four peptides, especially Ser-Gln-Ser-Pro-Ala, could be potential drugs for treating diabetes. (C) 2015 Elsevier Inc. All rights reserved.

分类号: Q5

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