An approach for long-term preservation of Blumeria graminis f. sp tritici

文献类型： 外文期刊

第一作者： Gong, Shuangjun

作者： Gong, Shuangjun;Yang, Lijun;Xiang, Libo;Shi, Wenqi;Xue, Minfeng;Zeng, Fansong;Zhang, Xuejiang;Wang, Hua;Yu, Dazhao

作者机构：

关键词： Blumeria graminis f. sp tritici;Cryopreservation;Genetic stability;Silica gel;Phenotype

期刊名称：TROPICAL PLANT PATHOLOGY （ 影响因子：1.488； 五年影响因子：1.675 ）

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年卷期：

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收录情况： SCI

摘要： Blumeria graminis f. sp. tritici (Bgt) is an obligate parasite that only infects living tissues of wheat (Triticum aestivum L.). Long term preservation of Bgt is useful for genetic studies but it is challenging due to difficulty of artificial cultivation. In this study a simple protocol was developed in which desiccating the conidia using crystals (3-6 mm in diameter) of silica gel allowed viable storage for 12 months. The conidia were mixed with silica gel, dried for 5 h at 23 A degrees C, and then stored at -80 A degrees C. The preserved Bgt isolates still maintained their viability after 12 months and successfully infected detached leaves of wheat seedlings. Analysis of 20 Bgt isolates revealed that the cryopreservation process had no effect on two key phenotypic variants: virulence against 12 near-isogenic wheat cultivars and their sensitivity to the fungicide triadimefon. DNA sequencing analysis confirmed that the nucleotide sequences of the 1.4 alpha-demethylase inhibitor (cyp51), chitin synthase 1 (chs1), and beta-tubulin (tub2) genes had not changed and still contained the corresponding single nucleotide mutations. These results indicate that the silica gel-based desiccation protocol is suitable for the long term preservation of large numbers of Bgt isolates.

分类号： Q94

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