iTRAQ-based quantitative proteomic analysis of Macrobrachium rosenbergii hemocytes during Spiroplasma eriocheiris infection

文献类型： 外文期刊

第一作者： Hou, Libo

作者： Hou, Libo;Xiu, Yunji;Wang, Jian;Liu, Yuhan;Gu, Wei;Wang, Wen;Meng, Qingguo;Xiu, Yunji;Liu, Xiaoqian;Hou, Libo;Xiu, Yunji;Wang, Jian;Liu, Yuhan;Gu, Wei;Wang, Wen;Meng, Qingguo

作者机构：

关键词： Spiroplasma eriocheiris;Macrobrachium rosenbergii;Hemocytes;iTRAQ;Proteomics

期刊名称：JOURNAL OF PROTEOMICS （ 影响因子：4.044； 五年影响因子：4.02 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： Spiroplasma eriocheiris, as a novel aquaculture pathogen, has led into catastrophic economic losses in aquaculture. The Macrobrachium rosenbergii hemocytes were major target cells in S. eriocheiris infection. Our study was designed to examine the hemocytes' immune response at the protein levels. The differential proteomes of the prawn hemocytes were analyzed immediately prior to injection with the pathogen, and at 192 h post-injection by isobaric tags for relative and absolute quantization (iTRAQ) labeling, followed by liquid chromatogramphytandem mass spectrometry (LC MS/MS). A total of 69 differentially expressed proteins were identified. Forty-nine proteins were up-regulated and 20 proteins were down-regulated resulting from a S. eriocheiris infection. Up-regulated proteins included vertebrate gliacolin-like protein, vitellogenin, Gram-negative binding protein 1, alpha2 macroglobulin isoform 2 (a2M), etc. Down-regulated proteins, involved with beta-1,3-glucan-binding protein (BGBP), immunoglobulin like, Rab7, lipopolysaccharide and beta-1,3-glucan (LGBP), actin-related protein, etc. Selected bioactive factors (tachylectin, alpha 2M and vitellogenin, BGBP, C-type lectin, LGBP and Rab7) were verified by their immune roles in the S. eriocheiris infection using real-time PCR. The present work could serve as a basis for future studies on the proteins implicated in the susceptibility/resistance of M. rosenbergii to S. eriocheiris, as well as contribute to our understanding of disease processes in prawns.

分类号： Q51

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