Physiological and morphological changes in Botrytis cinerea induced by the plant alkaloid camptothecin

文献类型： 外文期刊

第一作者： Mao, Shengfeng

作者： Mao, Shengfeng;Lei, Peng;Zhang, Liqin;Luo, Jinyan;Wu, Zhiyi;Mao, Shengfeng;Ge, Mengyu;Li, Bin;Wang, Yanli;Sun, Guochang

作者机构：

关键词： B. cinerea;Camptothecin;Enzyme activity;Gene expression;Mechanism

期刊名称：TROPICAL PLANT PATHOLOGY （ 影响因子：1.488； 五年影响因子：1.675 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Botrytis cinerea causes diseases in plants that lead to severe crop losses. This study aimed to evaluate the physiological and morphological changes and gene expression induced by camptothecin, a monoterpene indole alkaloid, for the control of B. cinerea. Using a single concentration of 8.0 mu g/mL, which showed to reduce 50 % of the dry weight of fungal mycelia, the alkaloid caused a 25 to 30 % reduction in colony diameter from 24 to 72 h of incubation and 34 to 54.7 % reduction in fungal biomass, from 24 to 96 h of incubation. Furthermore, changes in mycelial morphology were noticed based on both scanning and transmission electron microscopic observations. POD activity was reduced by 49.1 %, but PAL and SOD activity were not affected. Moreover, q-PCR analysis showed that the expression of erg27, os1, and SDHB were not affected, but the expression of cytb increased significantly, thus suggesting the involvement of this respiration-related gene in the response of B. cinerea to camptothecin. In summary, the alkaloid has the potential to improve the control of gray mold caused by B. cinerea.

分类号： Q94

  • 相关文献

[1]Response of the enzymes to nitrogen applications in cotton fiber (Gossypium hirsutum L.) and their relationships with fiber strength. Wang YouHua,Feng Ying,Xu NaiYin,Chen BingLin,Ma RongHui,Zhou ZhiGuo,Xu NaiYin. 2009

[2]Sucrose and citric acid accumulations in melon genotypes with different sugar and acid contents. Tang, Mi,Zhang, Bao-cai,Xie, Jun-jun,Bie, Zhi-long,Wu, Ming-zhu,Yi, Hong-ping,Feng, Jong-xin,Tang, Mi. 2012

[3]Molecular characterization, expression and activity of glucokinase in golden pompano, Trachinotus ovatus: Response of its expression to carbohydrate in the diet. Zhou, Chuanpeng,Wang, Jun,Wang, Yun,Lin, Heizhao,Huang, Zhong,Yu, Wei. 2018

[4]The effect of dietary folic acid on biochemical parameters and gene expression of three heat shock proteins (HSPs) of blunt snout bream (Megalobrama amblycephala) fingerling under acute high temperature stress. Sesay, Daniella Fatmata,Habte-Tsion, Habte-Michael,Zhou, Qunlan,Ren, Mingchun,Xie, Jun,Liu, Bo,Sesay, Daniella Fatmata,Zhou, Qunlan,Ren, Mingchun,Xie, Jun,Liu, Bo,Chen, Ruli,Pan, Liangkun.

[5]A manganese superoxide dismutase (MnSOD) from ark shell, Scapharca broughtonii: Molecular characterization, expression and immune activity analysis. Zheng, Libing,Wu, Biao,Liu, Zhihong,Tian, Jiteng,Zhou, Liqing,Sun, Xiujun,Yang, Aiguo,Zheng, Libing,Yu, Tao.

[6]Dynamic characteristics of enzymes and transcriptome related to sugar metabolism and accumulation in sweet and non-sweet watermelon fruits. Xu, Y.,Guo, S.,Liu, J.,He, H.,Zhang, H.,Ren, Y.,Sun, H.,Gong, G.,Fei, Z.,Zheng, Y.,Huang, M.,Zhong, S.,Liu, J.. 2012

[7]Characterization of Sugar Contents and Sucrose Metabolizing Enzymes in Developing Leaves of Hevea brasiliensis. Zhu, Jinheng,Qi, Jiyan,Fang, Yongjun,Xiao, Xiaohu,Lan, Jixian,Tang, Chaorong,Zhu, Jinheng,Lan, Jixian,Tang, Chaorong,Li, Jiuhui. 2018

[8]Effects of norfioxacin on hepatic genes expression of P450 isoforms (CYP1A and CYP3A), GST and P-glycoprotein (P-gp) in Swordtail fish (Xiphophorus Helleri). Liang, Ximei,Wang, Lan,Ou, Ruikang,Nie, Xiangping,Yang, YuFeng,Nie, Xiangping,Liang, Ximei,Wang, Fang,Li, Kaibin.

[9]Characterisation of the phytase gene in trifoliate orange (Poncirus trifoliata (L.) Raf.) seedlings. Shu, Bo,Wang, Peng,Xia, Ren-Xue,Shu, Bo,Shu, Bo.

[10]The effect of different temperatures on browning incidence and phenol compound metabolism in fresh-cut lotus (Nelumbo nucifera G.) root. Min, Ting,Xie, Jun,Zheng, Menglin,Yi, Yang,Hou, Wenfu,Wang, Limei,Ai, Youwei,Wang, Hongxun,Yi, Yang,Hou, Wenfu,Wang, Hongxun.

[11]Synthesis and Insecticidal Activity of Novel Camptothecin Derivatives Containing Analogs of Chrysanthemic Acid Moieties. Zhang Lan,Cao Li-dong,Xie Ru-liang,Zhang Yan-ning,He Wei-zhi,Jiang Hong-yun. 2014

[12]Evaluation of insecticidal activity of camptothecin analogs against Brontispa longissima. Yang, Liu,Zhang, Zhi-Jun,Liu, Ying-Qian,Zhao, Chun-Yan,Wang, Mei-Juan,Nan, Xiang,Feng, Gang.

[13]Structures of the Ozonolysis Products and Ozonolysis Pathway of Aflatoxin B-1 in Acetonitrile Solution. Diao, Enjie,Shan, Changpo,Hou, Hanxue,Dong, Haizhou,Wang, Shanshan,Li, Minghua. 2012

[14]The Mechanism by Which Dodecyl Dimethyl Benzyl Ammonium Chloride Increased the Toxicity of Chlorpyrifos to Spodoptera exigua. Cui, Li,Yuan, Huizhu,Yang, Daibin,Rui, Changhui,Mu, Wei. 2017

[15]Advances in characterisation and biological activities of chitosan and chitosan oligosaccharides. Zou, Pan,Yang, Xin,Wang, Jing,Zhang, Yanxin,Liu, Guangyang,Zou, Pan,Yang, Xin,Wang, Jing,Li, Yongfei,Yu, Hailong,Zhang, Yanxin,Liu, Guangyang,Wang, Jing,Li, Yongfei,Yu, Hailong. 2016

[16]Potential use of anaerobically digested manure slurry to suppress Phytophthora root rot of chilli pepper. Cao, Yun,Chang, Zhizhou,Wang, Jidong,Ma, Yan,Yang, Hao,Fu, Guangqin. 2014

[17]In vitro Antimicrobial Activities and Mechanism of 1-Octen-3-ol against Food-related Bacteria and Pathogenic Fungi. Li, Qiang,Chen, Zuqin,Xiong, Chuan,Li, Qiang,Huang, Wenli,Li, Shuhong,Chen, Cheng. 2017

[18]Chlorination of parabens: reaction kinetics and transformation product identification. Mao, Qianhui,Wang, Wei,Hu, Zhenhu,Yuan, Shoujun,Ji, Feng,Wang, Qiquan,Yuan, Shoujun. 2016

[19]Synthesis, Structure and Mechanism of a Lithium Complex with Hexanuclear Cage Structure. Wang Li,Xue Xiao-Yan,Huang Shu-Ping,Chen Xia,Chen Xia,Chang Gong-Min. 2011

[20]Mechanism and Effective Factors of Acyl Migration of Lysophospholipid. Zhang Kangyi,Qu Lingbo,Zhang Kangyi,Zhang Lixia,Wang Xingguo,Liu Yuanfa. 2014

作者其他论文 更多>>

微信小程序