Construction of a sequence-based bin map and mapping of QTLs for downy mildew resistance at four developmental stages in Chinese cabbage (Brassica rapa L. ssp pekinensis)

文献类型： 外文期刊

第一作者： Yu, Shuancang

作者： Yu, Shuancang;Su, Tongbing;Zhi, Shenghua;Zhang, Fenglan;Wang, Weihong;Zhang, Deshuang;Zhao, Xiuyun;Yu, Yangjun;Yu, Shuancang;Su, Tongbing;Zhi, Shenghua;Zhang, Fenglan;Wang, Weihong;Zhang, Deshuang;Zhao, Xiuyun;Yu, Yangjun;Yu, Shuancang;Su, Tongbing;Zhi, Shenghua;Zhang, Fenglan;Wang, Weihong;Zhang, Deshuang;Zhao, Xiuyun;Yu, Yangjun

作者机构：

关键词： Brassica rapa ssp pekinensis;Bin map;Downy mildew;Developmental stage;Quantitative trait loci;SNP

期刊名称：MOLECULAR BREEDING （ 影响因子：2.589； 五年影响因子：2.75 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Specific-locus amplified fragment sequencing is a high-resolution method for genetic mapping, genotyping, and single nucleotide polymorphism (SNP) marker discovery. Previously, a major QTL for downy mildew resistance, BraDM, was mapped to linkage group A08 in a doubled-haploid population derived from Chinese cabbage lines 91-112 and T12-19. The aim of the present study was to improve the linkage map and identify the genetic factors involved in downy mildew resistance. We detected 53,692 high quality SLAFs, of which 7230 were polymorphic, and 3482 of the polymorphic markers were used in genetic map construction. The final map included 1064 bins on ten linkage groups and was 858.98 cM in length, with an average inter-locus distance of 0.81 cM. We identified six QTLs that are involved in downy mildew resistance. The four major QTLs, sBrDM8, yBrDM8, rBrDM8, and hBrDM8, for resistance at the seedling, young plant, rosette, and heading stages were mapped to A08, and are identical to BraDM. The two minor resistance QTLs, rBrDM6 (A06) and hBrDM4 (A04), were active at the rosette and heading stages. The major QTL sBrDM8 defined a physical interval of similar to 228 Kb on A08, and a serine/threonine kinase family gene, Bra016457, was identified as the possible candidate gene. We report here the first high-density bin map for Chinese cabbage, which will facilitate mapping QTLs for economically important traits and SNP marker development. Our results also expand knowledge of downy mildew resistance in Chinese cabbage and provide three SNP markers (A08-709, A08-028, and A08-018) that we showed to be effective when used in MAS to breed for downy mildew resistance in B. rapa.

分类号： Q94

  • 相关文献

[1]QTL mapping for resistance to SCMV in chinese maize germplasm. Zhang, SH,Li, XH,Wang, ZH,George, ML,Jeffers, D,Wang, FG,Liu, XD,Li, MS,Yuan, LX. 2003

[2]QTL effects and epistatic interaction for flowering time and branch number in a soybean mapping population of JapanesexChinese cultivars. Yang Guang,Xie Fu-ti,Zhai Hong,Wu Hong-yan,Zhang Xing-zheng,Wang Ya-ying,Li Yu-qiu,Hu Bo,Wang Lu,Xia Zheng-jun,Zhang Xing-zheng,Wang Ya-ying,Li Yu-qiu,Wang Lu,Yang Guang,Lu Shi-xiang,Wen Zi-xiang,Wang De-chun,Wang Shao-dong,Harada, Kyuya. 2017

[3]Identification of a major quantitative trait locus for resistance to maize rough dwarf virus in a Chinese maize inbred line X178 using a linkage map based on 514 gene-derived single nucleotide polymorphisms. Shi, Li-yu,Hao, Zhuan-fang,Weng, Jian-feng,Xie, Chuan-xiao,Liu, Chang-lin,Zhang, De-gui,Li, Ming-shun,Bai, Li,Li, Xin-hai,Zhang, Shi-huang.

[4]Distribution of Baseline Sensitivities to Natural Product Physcion Among Isolates of Sphaerotheca fuliginea and Pseudoperonospora cubensis. Yang, X. J.,Ni, H.,Yang, X. J.,Yang, L. J.,Zeng, F. S.,Xiang, L. B.,Wang, S. N.,Yu, D. Z..

[5]The miRNAs and their regulatory networks responsible for pollen abortion in Ogura-CMS Chinese cabbage revealed by high-throughput sequencing of miRNAs, degradomes, and transcriptomes. Wei, Xiaochun,Yao, Qiuju,Yuan, Yuxiang,Zhao, Yanyan,Zhang, Qiang,Wang, Zhiyong,Jiang, Wusheng,Zhang, Xiaowei,Zhang, Xiaohui,Li, Xixiang,Wei, Fang. 2015

[6]Genome-wide identification and characterization of aquaporin genes (AQPs) in Chinese cabbage (Brassica rapa ssp pekinensis). Tao, Peng,Zhong, Xinmin,Li, Biyuan,Wang, Wuhong,Yue, Zhichen,Lei, Juanli,Huang, Xiaoyun,Guo, Weiling,Huang, Xiaoyun.

[7]Hybrid Purity Testing of Brassica rapa Using SSR Marker Technology. Li, Li,Liu, Ling,Wu, Ping,Xu, Xiulan,Li, Li,Liu, Ling,Zhang, Deshuang,Wu, Ping,Zhang, Fenglan,Xu, Xiulan,Li, Li,Liu, Ling,Zhang, Deshuang,Wu, Ping,Zhang, Fenglan,Xu, Xiulan,Li, Li,Liu, Ling,Zhang, Deshuang,Wu, Ping,Zhang, Fenglan,Xu, Xiulan,Li, Li,Liu, Ling,Zhang, Deshuang,Wu, Ping,Zhang, Fenglan,Xu, Xiulan.

[8]Molecular cloning and characterization of kiss1 in Brandt's voles (Lasiopodomys brandtii). Liu, Lan,Guo, Cong,Chen, Yan,Liu, Lan,Li, Zhengguang,Wang, Dawei,Li, Ning,Song, Ying,Liu, Xiaohui. 2017

[9]Expression of cadherin, aminopeptidase N and alkaline phosphatase genes in Cry1Ac-susceptible and Cry1Ac-resistant strains of Plutella xylostella (L.). Yang, Z-X.,Wu, Q-J.,Wang, S-L.,Chang, X-L.,Wang, J-H.,Guo, Z-J.,Lei, Y-Y.,Xu, B-Y.,Zhang, Y-J.,Yang, Z-X.. 2012

[10]Sensitivity of Bemisia Tabaci (Hemiptera: Aleyrodidae) to Several New Insecticides in China: Effects of Insecticide Type and Whitefly Species, Strain, and Stage. Xie, Wen,Liu, Yang,Wang, Shaoli,Wu, Qingjun,Pan, Huipeng,Yang, Xin,Guo, Litao,Zhang, Youjun. 2014

[11]Stage-Specific Expression of Resistance to Different Acaricides in Four Field Populations of Tetranychus urticae (Acari: Tetranychidae). Tang, Xiaofeng,Zhang, Youjun,Wu, Qingjun,Xie, Wen,Wang, Shaoli.

[12]Impact of epistasis and QTL x environment interaction on the accumulation of seed mass of soybean (Glycine max L. Merr.). Han, Yingpeng,Teng, Weili,Sun, Desheng,Du, Yuping,Li, Wenbin,Qiu, Lijuan,Han, Yingpeng,Xu, Xiangling.

[13]Large-scale screening for maize drought resistance using multiple selection criteria evaluated under water-stressed and well-watered environments. Hao, Zhuanfang,Xie, Chuanxiao,Li, Xinhai,Zhang, Shihuang,Lu, Yanli,Crossa, Jose,Araus, Jose-Luis,Taba, Suketoshi,Xu, Yunbi,Lu, Yanli,Gao, Shibin,Pan, Guangtang,Rong, Tingzhao,Vivek, Bindiganavile S.,Magorokosho, Cosmos,Mugo, Stephen,Makumbi, Dan,Xu, Yunbi.

[14]Automated detection and identification of white-backed planthoppers in paddy fields using image processing. Yao Qing,Chen Guo-te,Wang Zheng,Zhang Chao,Yang Bao-jun,Tang Jian. 2017

[15]Cloning and differential expression of five heat shock protein genes associated with thermal stress and development in the polyphagous predatory mite Neoseiulus cucumeris (Acari: Phytoseiidae). Chen, Wei,Zhang, Min,Zhao, Yunlong,Wu, Wenjing,Zhang, Guren,Li, Dunsong.

[16]How to Compare the Lower Developmental Thresholds. Shi, Peijian,Ge, Feng,Men, Xingyuan.

[17]A reappraisal of the critical nitrogen concentration of wheat and its implications on crop modeling. Zhao, Zhigan,Wang, Zhimin,Zang, Hecang,Liu, Yunpeng,Zhao, Zhigan,Wang, Enli,Zhao, Zhigan,Wang, Enli,Zang, Hecang,Angus, John F.,Angus, John F..

[18]Toxicity of biocide GCSC-BtA on arthropod pests under different temperature conditions. Zhu, YJ,Sengonca, C,Liu, B.

[19]Characterization of a TIR-NBS-LRR gene associated with downy mildew resistance in grape. Fan, J. J.,Xu, X.,Ruan, Y. Y.,Zhu, Y. S.,Cui, Z. H.,Zhang, L. J.,Wang, P.,Liu, K.. 2015

[20]Breeding disease-resistant Hami-melon. Wu, MZ,Yi, HP,Zhai, WQ,Feng, JX. 2004

作者其他论文 更多>>

微信小程序