A single cytosine deletion in the OsPLS1 gene encoding vacuolar-type H+-ATPase subunit A1 leads to premature leaf senescence and seed dormancy in rice

文献类型： 外文期刊

第一作者： Yang, Xi

作者： Yang, Xi;Gong, Pan;Li, Kunyu;Cheng, Fangmin;Pan, Gang;Huang, Fudeng

作者机构：

关键词： Oryza sativa L;OsPLS1;premature leaf senescence;ROS;SA;seed dormancy;vacuolar-type H+-ATPase

期刊名称：JOURNAL OF EXPERIMENTAL BOTANY （ 影响因子：6.992； 五年影响因子：7.86 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Leaf senescence is a programmed developmental process orchestrated by many factors, but its molecular regulation is not yet fully understood. In this study, a novel Oryza sativa premature leaf senescence mutant (ospls1) was examined. Despite normal development in early seedlings, the ospls1 mutant leaves displayed lesion-mimics and early senescence, and a high transpiration rate after tillering. The mutant also showed seed dormancy attributable to physical (defect of micropyle structure) and physiological (abscisic acid sensitivity) factors. Using a map-based cloning approach, we determined that a cytosine deletion in the OsPLS1 gene encoding vacuolar H+-ATPase subunit A1 (VHA-A1) underlies the phenotypic abnormalities in the ospls1 mutant. The OsPSL1/VHA-A1 transcript levels progressively declined with the age-dependent leaf senescence in both the ospls1 mutant and its wild type. The significant decrease in both OsPSL1/VHA-A1 gene expression and VHA enzyme activity in the ospls1 mutant strongly suggests a negative regulatory role for the normal OsPLS1/VHA-A1 gene in the onset of rice leaf senescence. The ospls1 mutant featured higher salicylic acid (SA) levels and reactive oxygen species (ROS) accumulation, and activation of signal transduction by up-regulation of WRKY genes in leaves. Consistent with this, the ospls1 mutant exhibited hypersensitivity to exogenous SA and/or H2O2. Collectively, these results indicated that the OsPSL1/VAH-A1 mutation played a causal role in premature leaf senescence through a combination of ROS and SA signals. To conclude, OsPLS1 is implicated in leaf senescence and seed dormancy in rice.

分类号： Q94

  • 相关文献

[1]Response of the vacuolar ATPase subunit E to RNA interference and four chemical pesticides in Leptinotarsa decemlineata (Say). Fu, Kai-Yun,Lu, Feng-gong,Liu, Xin-ping,Li, Guo-Qing,Guo, Wen-Chao.

[2]The Wheat Bax Inhibitor-1 Protein Interacts with an Aquaporin TaPIP1 and Enhances Disease Resistance in Arabidopsis. Lu, Pan-Pan,Yu, Tai-Fei,Zheng, Wei-Jun,Zhou, Yong-Bin,Xi, Ya-Jun,Lu, Pan-Pan,Yu, Tai-Fei,Chen, Ming,Zhou, Yong-Bin,Chen, Jun,Ma, You-Zhi,Xu, Zhao-Shi. 2018

[3]Arabidopsis FHY3 and FAR1 Regulate Light-Induced myo-Inositol Biosynthesis and Oxidative Stress Responses by Transcriptional Activation of MIPS1. Ma, Lin,Tian, Tian,Li, Gang,Lin, Rongcheng,Deng, Xing-Wang,Wang, Haiyang. 2016

[4]NPR1-dependent salicylic acid signaling is not involved in elevated CO2-induced heat stress tolerance in Arabidopsis thaliana. Li, Xin,Ahammed, Golam Jalal,Li, Xin,Yu, Jingquan,Shi, Kai. 2015

[5]Preliminary study on phytoalexin induction in cucumber. Wang, JB,Wang, BC,Zhao, HC,Li, BJ.

[6]Isolation, expression and functional analysis of a putative RNA-dependent RNA polymerase gene from maize (Zea mays L.). Wang, Guoying,He, Junguang,Dong, Zhigang,Jia, Zhiwei,Wang, Jianhua.

[7]Microarray-based gene expression analysis of strong seed dormancy in rice cv. N22 and less dormant mutant derivatives. Wu, Tao,Yang, Chunyan,Ding, Baoxu,Feng, Zhiming,Wang, Qian,He, Jun,Jiang, Ling,Wan, Jianmin,Wan, Jianmin,Tong, Jianhua,Xiao, Langtao. 2016

[8]Identification of QTLs for seed dormancy in rice (Oryza sativa L.). Xie, Kun,Jiang, Ling,Lu, BingYue,Yang, ChunYan,Li, LinFang,Liu, Xi,Zhang, Long,Zhao, ZhiGang,Wan, JianMin,Wan, JianMin.

[9]Mutations of genes in synthesis of the carotenoid precursors of ABA lead to pre-harvest sprouting and photo-oxidation in rice. Fang, Jun,Chai, Chenglin,Li, Chunlai,Tang, Jiuyou,Huang, Zejun,Guo, Xiaoli,Sun, Changhui,Liu, Min,Wang, Yiqin,Cheng, Zhukuan,Chu, Chengcai,Fang, Jun,Chai, Chenglin,Li, Chunlai,Tang, Jiuyou,Huang, Zejun,Guo, Xiaoli,Sun, Changhui,Liu, Min,Wang, Yiqin,Cheng, Zhukuan,Chu, Chengcai,Fang, Jun,Chai, Chenglin,Li, Chunlai,Tang, Jiuyou,Huang, Zejun,Guo, Xiaoli,Qian, Qian,Sun, Lei,Zhang, Yan,Lu, Qingtao,Lu, Congming,Han, Bin,Chen, Fan.

[10]Transcriptomics Analysis Identified Candidate Genes Colocalized with Seed Dormancy QTLs in Rice (Oryza sativa L.). Qin, Huaide,Xie, Kun,Jiang, Ling,Wan, Jianmin,Wu, Fuqing,Cheng, Zhijun,Guo, Xiuping,Zhang, Xin,Wang, Jie,Lei, Cailin,Wang, Jiulin,Mao, Long,Wan, Jianmin.

[11]Exogenous auxin regulates multi-metabolic network and embryo development, controlling seed secondary dormancy and germination in Nicotiana tabacum L.. Li, Zhenhua,Wang, Jianhua,Li, Zhenhua,Zhang, Jie,Zhao, Jiehong,Ren, Xueliang,Liu, Yiling,Fu, Junjie,Wang, Guoying. 2016

[12]Genome-Wide Association Study of Seed Dormancy and the Genomic Consequences of Improvement Footprints in Rice (Oryza sativa L.). Lu, Qing,Niu, Xiaojun,Zhang, Mengchen,Wang, Caihong,Xu, Qun,Feng, Yue,Yang, Yaolong,Wang, Shan,Yuan, Xiaoping,Yu, Hanyong,Wang, Yiping,Wei, Xinghua,Lu, Qing,Chen, Xiaoping,Liang, Xuanqiang,Lu, Qing,Chen, Xiaoping,Liang, Xuanqiang. 2018

[13]Genetic dissection of the seed dormancy trait in cultivated rice (Oryza sativa L.). Wan, JM,Jiang, L,Tang, JY,Wang, CM,Hou, MY,Jing, W,Zhang, L.

[14]Haplotype analysis of Viviparous-1 gene in CIMMYT elite bread wheat germplasm. Sun, Y. W.,Li, S. M.,Chen, X. M.,Xia, L. Q.,Jones, H. D.,Shewry, P. R.,Yang, Y.,Dreisigacker, S..

[15]DELAY OF GERMINATION1 (DOG1) regulates both seed dormancy and flowering time through microRNA pathways. Bradford, Kent J.,Wei, Shouhui.

[16]Variation of the OsGI intron and its phenotypic associations in Oryza rufipogon Griff. and Oryza sativa L.. Chen, Z.,Pei, X.,Jia, S.,Wang, F.,Yuan, Q.,Wu, H.,Peng, Y.. 2013

[17]Seed losses at harvest and seed persistence of oilseed rape (Brassica napus) in different cultural conditions in Chinese farming systems. Zhu, Y. M.,Ma, K. P.,Wei, W.,Mi, X. C.,Li, Y. D.,Colbach, N.. 2012

[18]Proteome changes associated with dormancy release of Dongxiang wild rice seeds. Xu, Heng-Heng,Liu, Shu-Jun,Wang, Wei-Qing,Song, Song-Quan,Song, Shun-Hua,Moller, Ian Max.

[19]A novel role for histone methyltransferase KYP/SUVH4 in the control of Arabidopsis primary seed dormancy. Zheng, Jian,Chen, Fengying,Wang, Zhi,Cao, Hong,Li, Xiaoying,Liu, Yongxiu,Zheng, Jian,Wang, Zhi,Deng, Xin,Soppe, Wim J. J.,Li, Yong.

[20]IDENTIFICATION OF A SET OF RFLP PROBES FOR SUBSPECIES DIFFERENTIATION IN ORYZA-SATIVA L. QIAN, HR,ZHUANG, JY,LIN, HX,LU, J,ZHENG, KL. 1995

作者其他论文 更多>>

微信小程序