Cloning and Transformation of EeHKT1;4 Gene from Elytrigia elongata

文献类型： 外文期刊

第一作者： Meng, Lin

作者： Meng, Lin;Zhang, Lin;Guo, Qiang;Li, Shan-Shan;Mao, Pei-Chun;Tian, Xiao-Xia

作者机构：

关键词： Clone;EeHKT1;4 gene;Elytrigia elongata (Host) Nevski;plant expression vector;transformation;transgenic tobacco

期刊名称：PROTEIN AND PEPTIDE LETTERS （ 影响因子：1.89； 五年影响因子：1.528 ）

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收录情况： SCI

摘要： The EeHKT1;4 gene was firstly cloned from Elytrigia elongata by RT-PCR technique with 1977 bp full-length cDNA encoding 1722 bp open reading frame (ORF) and 573 amino acids. The PCR fragment of EeHKT1;4 gene was inserted into the binary vector pBI121 and got the resulted expression vector, which named pBI121-35S-EeHKT1;4-Nos. The vector was further transformed into the agrobacterium EHA105, and then EeHKT1;4 gene was transferred into tobacco by the Agrobaterium-mediated genetic transformation method. The results showed that the target gene was inserted into the genomes of tobacco and expressed. Therefore, the transgenic tobacco (T-0) plants overexpressing EeHKT1;4 gene were successfully obtained in this study. And EeHKT1;4 reduces Na+ concentration in the leaves of T-0 plants, thereby plays a central role in protecting plant leaves from salinity stress.

分类号： Q51

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