Construction of a Rapid Feather-Degrading Bacterium by Overexpression of a Highly Efficient Alkaline Keratinase in Its Parent Strain Bacillus amyloliquefaciens K11
文献类型: 外文期刊
第一作者: Yang, Lian
作者: Yang, Lian;Wang, Hui;Lv, Yi;Bai, Yingguo;Luo, Huiying;Shi, Pengjun;Huang, Huoqing;Yao, Bin
作者机构:
关键词: Bacillus amyloliquefaciens K11;keratinase;extreme alkaline;gene expression
期刊名称:JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY ( 影响因子:5.279; 五年影响因子:5.269 )
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收录情况: SCI
摘要: Keratinase is essential to degrade the main feather component, keratin, and is of importance for wide industrial applications. In this study, Bacillus amyloliquefaciens strain K11 was found to have significant feather-degrading capacity (completely degraded whole feathers within 24 h). The keratinase encoding gene, kerK, was expressed in Bacillus subtilis SCK6. The purified recombinant KerK showed optimal activity at 50 degrees C and pH 11.0 and degraded whole feathers within 0.5 h in the presence of DTT. The recombinant plasmids harboring kerK were extracted from B. subtilis SCK6 and transformed into B. amyloliquefaciens K11. As a result, the recombinant B. amyloliquefaciens K11 exhibited enhanced feather-degrading capacity with shortened reaction time within 12 h and increased keratinolytic activity (1500 U/mL) by 6-fold. This efficient and rapid feather-degrading character makes the recombinant strain of B. amyloliquefaciens K11 have potential for applications in feather meal preparation and waste feather disposal.
分类号: R15`S
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