Development and antigenic characterization of three recombinant proteins with potential for Glasser's disease prevention

文献类型： 外文期刊

第一作者： Li, Miao

作者： Li, Miao;Li, Chunling;Song, Shuai;Kang, Huahua;Yang, Dongxia;Li, Miao;Li, Chunling;Song, Shuai;Kang, Huahua;Yang, Dongxia;Li, Miao;Li, Chunling;Song, Shuai;Kang, Huahua;Yang, Dongxia;Li, Miao;Li, Guoqing

作者机构：

关键词： Haemophilus parasuis;Outer membrane proteins;Immune responses;Protection;Vaccine candidate

期刊名称：VACCINE （ 影响因子：3.641； 五年影响因子：3.816 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Haemophilus parasuis is the causative agent of Glasser's disease, which causes high morbidity and mortality in piglets, leading to severe economic losses. The lack of a commercial vaccine against a broad spectrum of strains has limited the disease control. H. parasuis outer membrane proteins (OMPs) are potentially essential components for vaccine formulation. In this study, seven putative OMPs were selected from the annotated H. parasuis serovar 5 genome; they were predicted by bioinformatics and annotated as potential virulence-related factors. These proteins were cloned, expressed, and purified as His-tagged proteins. Antigenicity of the candidate proteins was assessed using Western blotting with convalescent sera against H. parasuis serovar 5. The immunogenicity of the seven OMPs was assessed in a guinea pig model. Except VacJ, all the other six recombinant proteins elicited a detectable antibody response. Anti sera against four of the selected proteins effectively killed the bacteria in vitro. Three proteins (Omp26, VacJ, and HAP5_0742) were found to confer significant protection against challenge with a lethal dose of H. parasuis in a guinea pig model. The results suggest that these three proteins have a strong potential to be vaccine candidates against Glasser's disease. (C) 2016 Elsevier Ltd. All rights reserved.

分类号： R392

  • 相关文献

[1]Identification of secreted proteins as novel antigenic vaccine candidates of Haemophilus parasuis serovar 5. Li, Miao,Li, Guoqing,Li, Miao,Song, Shuai,Yang, Dongxia,Li, Chunling,Li, Miao,Song, Shuai,Yang, Dongxia,Li, Chunling,Li, Miao,Song, Shuai,Yang, Dongxia,Li, Chunling.

[2]Evaluation of glutathione-binding protein A of Haemophilus parasuis as a vaccine candidate in a mouse model. Zhang, Yanhe,Li, Gang,Xie, Fang,Liu, Siguo,Wang, Chunlai. 2017

[3]Comparison of immune responses against foot-and-mouth disease virus induced by fusion proteins using the swine IgG heavy chain constant region or beta-galactosidase as a carrier of immunogenic epitopes. Li, GJ,Chen, WZ,Yan, WY,Zhao, K,Liu, MQ,Zhang, J,Fei, L,Xu, QX,Sheng, ZT,Lu, YG,Zheng, ZX.

[4]Immunogenic cross-reaction among outer membrane proteins of Gram-negative bacteria. Xu, CX,Wang, SY,Zhang, ZX,Peng, XX. 2005

[5]Immunoproteomic Analysis of Bordetella bronchiseptica Outer Membrane Proteins and Identification of New Immunogenic Proteins. Liu Yan,Qin Feng-yan,Bao Guo-lian,Chen Hui,Xiao Chen-wen,Wei Qiang,Ji Quan-an. 2014

[6]Preliminary Extraction and Identification of the 44.5 kDa Outer Membrane Proteins Isolated from Bovine Fusobacterium necrophorum (AB). Xu, Jing,Wang, Sun-Jie,Xu, Jing,Chen, Li-Zhi,Liu, Xiao-Ying,Feng, Er-Kai,Wang, Sun-Jie,Cao, Yue.

[7]Positive role for rApxIVN in the immune protection of pigs against infection by Actinobacillus pleuropneumoniae. Wang, Chunlai,Wang, Yong,Shao, Meili,Si, Wei,Liu, Huifang,Chang, Yuehong,Peng, Wei,Kong, Xiangang,Liu, Siguo,Shao, Meili.

[8]Differential proteomics of outer membrane proteins between virulent and avirulent strains of Riemerella anatipestifer. Liu Yan,Wei Qiang,Bao Guo-Lian,Ji Quan-An. 2008

[9]Characterization of Schistosoma japonicum estrogen-related receptor beta like 1 and immunogenicity analysis of the recombinant protein. Wu, Xiujuan,Zhao, Bin,Hong, Yang,Li, Xuezheng,Peng, Jinbiao,Zhang, Juan,Wang, Fei,Shi, Yaojun,Fu, Zhiqiang,Lin, Jiaojiao.

[10]Vibrio parahaemolyticus enolase is an adhesion-related factor that binds plasminogen and functions as a protective antigen. Jiang, Wei,Li, Xintong,Yi, Li,Liu, Yongjie,Jiang, Wei,Han, Xiangan,Wang, Quan,Ding, Chan.

[11]Experimental infection and comparative genomic analysis of a highly pathogenic PRRSV-HBR strain at different passage levels. Wei, Yanwu,Li, Shengbin,Huang, Liping,Tang, Qinghai,Liu, Jianbo,Liu, Dan,Wang, Yiping,Wu, Hongli,Liu, Changming. 2013

[12]Characterization and cross-protection evaluation of M949_1603 gene deletion Riemerella anatipestifer mutant RA-M1. Zou, Jiechi,Wang, Xiaolan,Ding, Chan,Tian, Mingxing,Han, Xiangan,Wang, Shaohui,Yu, Shengqing.

[13]Cloning, expression and characterization of protein disulfide isomerase of Schistosoma japonicum. Cao, Xiaodan,Hong, Yang,Zhang, Min,Han, Yanhui,Wu, Miaoli,Wang, Xinzhuo,Guo, Xiaoyong,Li, Changjian,Lu, Ke,Li, Hao,Fu, Zhiqiang,Lin, Jiaojiao,Lin, Jiaojiao,Zhang, Min,Han, Yanhui.

[14]Development and application of a loop-mediated isothermal amplification method for rapid detection of Haemophilus parasuis. Zhang, Jian-Min,Xu, Cheng-Gang,Guo, Li-Li,Zhang, Bin,Li, Jing-Yi,Chen, Ji-Dang,Fan, Hui-Ying,Liao, Ming,Shen, Hai-Yan. 2011

[15]Electrotransformation of Haemophilus parasuis with in vitro modified DNA based on a novel shuttle vector. Chen, Liping,Wu, Dongfang,Cai, Xuwang,Guo, Fengjuan,Xu, Xiaojuan,Chen, Huanchun,Chen, Liping,Blackall, P. J.. 2012

[16]Rapid detection of Haemophilus parasuis using cross-priming amplification and vertical flow visualization. Li, Chunling. 2018

[17]Concurrent highly pathogenic porcine reproductive and respiratory syndrome virus infection accelerates Haemophilus parasuis infection in conventional pigs. Wang, Jinbao,Yu, Jiang,Wang, Jinbao. 2012

[18]Development of a universal plate-agglutination test for detecting Haemophilus parasuis. Guo, Dingqian,Tang, Cheng,Hai, Quan,Yue, Hua,Shao, Guoqing. 2010

[19]Identification of novel Haemophilus parasuis serovar 5 vaccine candidates using an immunoproteomic approach. Li, Gang,Xie, Fang,Li, Jianjun,Liu, Jiao,Li, Dapeng,Zhang, Yanhe,Liu, Siguo,Wang, Chunlai,Langford, Paul R.,Li, Yanwen.

[20]Multilocus sequence typing and virulence analysis of Haemophilus parasuis strains isolated in five provinces of China. Wang, Liyan,Ma, Lina,Liu, Yongan,Gao, Pengcheng,Li, Youquan,Li, Xuerui,Liu, Yongsheng.

作者其他论文 更多>>
  • Comparative genomics-based insights into Pantoea ananatis strains, isolated from white spot diseased leaves of maize with plant growth-promoting attributes

    作者：Yang, Fenghuan;Li, Miao;Wu, Hanxiang;Yu, Chao;Liu, Wende;Chen, Huamin

    关键词：Pantoea ananatis; comparative genomics; evolution; environmental adaptation; maize white spot

  • EasyMetagenome: A user-friendly and flexible pipeline for shotgun metagenomic analysis in microbiome research

    作者：Bai, Defeng;Xun, Jiani;Ma, Chuang;Luo, Hao;Yang, Haifei;Hou, Huiyu;Lv, Hujie;Wan, Xiulin;Wang, Yao;Yousuf, Salsabeel;Zeng, Meiyin;Zhang, Tianyuan;Gao, Yunyun;Liu, Yong-Xin;Chen, Tong;Ma, Chuang;Yang, Haifei;Cao, Chen;Cao, Xiaofeng;Cui, Jianzhou;Deng, Yuan-Ping;Deng, Zhaochao;Yu, Hao;Zhang, Chunfang;Dong, Wenxin;Dong, Wenxue;Du, Juan;Fang, Qunkai;Fang, Wei;Fang, Yue;Luan, Yaning;Fu, Fangtian;Fu, Min;Fu, Yi-Tian;Gao, He;Ge, Jingping;Guo, Yuhao;Gong, Qinglong;Lou, Wenbo;Gu, Lunda;Yang, Li;Guo, Peng;Hai, Tang;Liu, Hao;He, Jieqiang;He, Zi-Yang;Huang, Can;Ji, Shuai;Jiang, ChangHai;Jiang, Gui-Lai;Jiang, Lingjuan;Jin, Ling N.;Li, Changchao;Kan, Yuhe;Kang, Da;Kou, Jin;Lam, Ka-Lung;Li, Chong;Li, Fuyi;Li, Liwei;Li, Miao;Li, Xin;Li, Ye;Li, Zheng-Tao;Zhu, Chengshuai;Liang, Jing;Mo, Jiayuan;Lin, Yongxin;Liu, Changzhen;Liu, Danni;Zhang, Jing;Chen, Shifu;Liu, Fengqin;Liu, Jia;Liu, Tianrui;Liu, Tingting;Wang, Xinlong;Liu, Xinyuan;Luo, Yuanyuan;Liu, Yaqun;Liu, Bangyan;Liu, Minghao;Lv, Hujie;Ma, Tengfei;Mai, Zongjiong;Niu, Dongze;Pan, Zhuo;Qi, Heyuan;Shi, Zhanyao;Song, Chunjiao;Sun, Fuxiang;Sun, Yan;Tian, Sihui;Wang, Guoliang;Wang, Hongyang;Wang, Hongyu;Wang, Huanhuan;Wang, Jing;Wang, Jun;Wang, Kang;Wang, Leli;Yao, Xiaofang;Wang, Shao-kun;Xiao, Zufei;Xing, Huichun;Xu, Yifan;Yang, Song;Yan, Shu-yan;Zhang, Yi-Bo;Yang, Yuanming;Lei, Yu;Yuan, Zhengrong;Zhang, Chunge;Zhang, Huimin;Zhang, Na;Zhang, Yupeng;Zhang, Zheng;Zhou, Mingda;Zhou, Yuanping;Zhu, Zhihao;Zhu, Lin;Zhu, Yue;Zou, Hongqin;Zuo, Anna;Dong, Wenxuan;Wen, Tao;Chen, Shifu;Chen, Shifu;Li, Guoliang

    关键词：metagenome; microbiome; microbiota; pipeline; visualization

  • QTL Mapping and Candidate Gene Analysis for Cotton Fiber Quality and Early Maturity Using F2 and F3 Generations

    作者：Jia, Xiaoyun;Zhu, Jijie;Zhao, Hongxia;Kong, Linglei;Wang, Shijie;Li, Miao;Wang, Guoyin

    关键词：upland cotton; fiber quality; early maturity; correlation analysis; quantitative trait loci; candidate gene

  • Genetic Diversity and Population Structure of Plenodomus biglobosus on Flixweed (Descurainia sophia) in Northwestern China

    作者：Luo, Tao;Si, Wei;Wu, Mingde;Zhang, Jing;Li, Guoqing;Yang, Long;Luo, Tao;Si, Wei;Wu, Mingde;Zhang, Jing;Li, Guoqing;Yang, Long;Jia, Donghai

    关键词：blackleg; flixweed; mating type; pennycress; Plenodomus biglobosus; population structure

  • Improved MobileVit deep learning algorithm based on thermal images to identify the water state in cotton

    作者：Jin, Kaijun;Zhang, Jihong;Liu, Ningning;Li, Miao;Ma, Zhanli;Wang, Zhenhua;Zhang, Jinzhu;Yin, Feihu;Jin, Kaijun;Zhang, Jihong;Liu, Ningning;Li, Miao;Ma, Zhanli;Wang, Zhenhua;Zhang, Jinzhu;Jin, Kaijun;Zhang, Jihong;Liu, Ningning;Li, Miao;Ma, Zhanli;Wang, Zhenhua;Zhang, Jinzhu;Jin, Kaijun;Zhang, Jihong;Liu, Ningning;Li, Miao;Ma, Zhanli;Wang, Zhenhua;Zhang, Jinzhu;Yin, Feihu

    关键词：Cotton; Water state identification; Computer vision; Improved MobileVit algorithm; Deep learning

  • Effect of dynamic pressure and emitter type on irrigation and fertigation uniformity of drip irrigation systems

    作者：Xing, Shaobo;Du, Kexin;Liu, Ningning;Li, Miao;Li, Wenhao;Zhang, Jihong;Zhang, Jinzhu;Wang, Zhenhua;Xing, Shaobo;Du, Kexin;Liu, Ningning;Li, Miao;Li, Wenhao;Zhang, Jihong;Zhang, Jinzhu;Wang, Zhenhua;Xing, Shaobo;Du, Kexin;Liu, Ningning;Li, Miao;Li, Wenhao;Zhang, Jihong;Zhang, Jinzhu;Wang, Zhenhua;Xing, Shaobo;Du, Kexin;Liu, Ningning;Li, Miao;Li, Wenhao;Zhang, Jihong;Zhang, Jinzhu;Wang, Zhenhua;Yin, Feihu

    关键词：Dynamic pressure; Drip irrigation; Drip tape type; Irrigation uniformity; Fertigation uniformity

  • Rosemary Extract Reduces Odor in Cats Through Nitrogen and Sulfur Metabolism by Gut Microbiota-Host Co-Modulation

    作者：Huang, Ziming;Li, Miao;He, Zhiqin;Yan, Xiliang;Wu, Yinbao;Wang, Yan;Yan, Xiliang;Wu, Yinbao;Wang, Yan;Yan, Xiliang;Wu, Yinbao;Wang, Yan;Mu, Peiqiang;Jiang, Jun;Wang, Xu

    关键词：rosemary extracts; pet cats; ammonia (NH3); hydrogen sulfide (H2S); odor reduction

微信小程序