Antibody-free colorimetric determination of total aflatoxins by mercury(II)-mediated aggregation of lysine-functionalized gold nanoparticles

文献类型： 外文期刊

第一作者： Du, Bibai

作者： Du, Bibai;Xiao, Can;Zhou, Ying;Wu, Li;Zhao, Hong;Yang, Jingkui;He, Yujian;Wang, Peilong;Su, Xiaoou;Wu, Li;He, Yujian

作者机构：

关键词： Spectrophotometry;Group specific assay;Visual test;Colloid;Transmission electron microscopy;Dynamic light scattering;Density functional theory;HPLC;Food analysis

期刊名称：MICROCHIMICA ACTA （ 影响因子：5.833； 五年影响因子：5.357 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Currently known immunoassays for aflatoxins (AFs) usually are not capable of simultaneous determination of total AFs (i.e., aflatoxin B-1, aflatoxin B-2, aflatoxin G(1), aflatoxin G(2)) due to the lack of group-specific antibodies. We are presenting here a colorimetric method for determination of total AFs by exploiting the Hg(II) ion-mediated aggregation of lysine-functionalized gold nanoparticles (Lys-AuNPs). AFs bind Hg(II) ion to form stable complexes, and this prevents the Hg(II) induced aggregation of Lys-AuNPs. Hence, the color change of solutions of AuNPs that occurs after aggregation of the AuNPs is suppressed. This effect was exploited to design a method for quantitation of AFs that can be detected by UV-Vis spectrophotometry (by measuring the ratio of absorbances at 725 nm and 525 nm), and even with bare eyes. The method has a very low detection limit (1.1 ppb) and is highly selective for AFs over other mycotoxins. Colorimetric analysis of rice samples by this method gave results that were in good agreement with those obtained by HPLC. To the best of our knowledge, this is the first method for visual detection of total AFs based on the distance-dependent optical properties of AuNPs.

分类号： O65`O655

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