Simultaneous preparation and characterization of three high-purity type B fumonisins from maize culture

文献类型： 外文期刊

第一作者： Guo, Wenbo

作者： Guo, Wenbo;Han, Zheng;Yang, Junhua;Rao, Qinxiong;Zhao, Zhihui

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期刊名称：ANALYTICAL METHODS （ 影响因子：2.896； 五年影响因子：2.716 ）

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收录情况： SCI

摘要： A reliable and large-scale preparative method was developed for the simultaneous preparation of high-purity fumonisin B-1 (FB1), fumonisin B-2 (FB2) and fumonisin B-3 (FB3) from maize culture inoculated with Fusarium moniliforme in this study. The targeted B-series fumonisins (FBs) were firstly extracted with acetonitrile/water (50 : 50, v/v) and then purified using MAX cartridges. The subsequent chromatographic preparative procedures on a Unitary C-18 column (250 mm x 10 mm, 5 mm) and a SB-CN column (250 mm x 9.4 mm, 5 mm) were performed to effectively eliminate the other interferents to obtain the high-purity compounds. The prepared compounds were clearly identified to be FB1, FB2 and FB3 using liquid chromatography-tandem mass spectrometry (LC-MS/MS) and nuclear magnetic resonance (NMR). The purities were 96.25 +/- 0.18%, 98.07 +/- 0.18% and 97.36 +/- 0.74% for FB1, FB2 and FB3, respectively, which were determined by the external standard LC-MS/MS method. Within 72 h, large amounts of high-purity FB1 (80 mg), FB2 (16 mg) and FB3 (16 mg) could be simultaneously prepared by the established approach.

分类号： O65

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