Development of an Enzyme-Linked Immunosorbent Assay Based on Fusion VP2332-452 Antigen for Detecting Antibodies against Aleutian Mink Disease Virus
文献类型： 外文期刊
第一作者： Chen, Xiaowei
作者： Chen, Xiaowei;Song, Cailing;Liu, Yun;Qu, Liandong;Liu, Dafei;Zhang, Yun;Liu, Ming
作者机构：
期刊名称：JOURNAL OF CLINICAL MICROBIOLOGY （ 影响因子：5.948； 五年影响因子：5.345 ）
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年卷期：
页码：
收录情况： SCI
摘要： For detection of Aleutian mink disease virus (AMDV) antibodies, an enzyme-linked immunosorbent assay (ELISA) was developed using the recombinant VP2(332-452) protein as an antigen. Counterimmunoelectrophoresis (CIEP) was used as a reference test to compare the results of the ELISA and Western blotting (WB); the specificity and sensitivity of the VP2(332-452) ELISA were 97.9% and 97.3%, respectively, which were higher than those of WB. Therefore, this VP2(332-452) ELISA may be a preferable method for detecting antibodies against AMDV.
分类号： R37
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