iTRAQ-based comparative proteomic analysis of excretory-secretory proteins of schistosomula and adult worms of Schistosoma japonicum

文献类型： 外文期刊

第一作者： Cao, Xiaodan

作者： Cao, Xiaodan;Fu, Zhiqiang;Han, Hongxiao;Han, Qian;Lu, Ke;Hong, Yang;Lin, Jiaojiao;Zhang, Min;Han, Yanhui;Lin, Jiaojiao

作者机构：

关键词： Schistosoma japonicum;Schistosomula;Adult worms;iTRAQ;Excretory/secretory proteins

期刊名称：JOURNAL OF PROTEOMICS （ 影响因子：4.044； 五年影响因子：4.02 ）

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收录情况： SCI

摘要： Schistosomiasis remains a serious public health problem with 200 million people infected and 779 million people at risk worldwide. The schistosomulum and adult worm are two stages of the complex lifecycle of Schistosoma japonicum and excretory/secretory proteins (ESPs) play a major role in host-parasite interactions. In this study, iTRAQ-coupled LC-MS/MS was used to investigate the proteome of ESPs obtained from schistosomula and adult worms of S.japonicum, and 298 differential ESPs were identified. Bioinformatics analysis of differential ESPs in the two developmental stages showed that 161 ESPs upregulated in schistosomula were associated with stress responses, carbohydrate metabolism and protein degradation, whereas ESPs upregulated in adult worms were mainly related to immunoregulation and purine metabolism. Recombinant heat shock protein 70 (HSP70) and thioredoxin peroxidase (TPx), two differential proteins identified in this study, were expressed. Further studies showed that rSjHSP70 and rSjTPx stimulated macrophages expressing high levels of the anti-inflammatory factors TGF-beta, IL-10 and Arg-1, and suppressed the expression of the pro-inflammatory cytokines TNF-alpha, IL-beta, IL-6 and iNOS in LPS-induced macrophages. This study provides new insights into the survival and development of schistosomes in the final host and helps identify vaccine candidates or new diagnostic reagents for schistosomiasis. (C) 2016 Elsevier B.V. All rights reserved.

分类号： Q51

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