Promising MS2 mediated virus-like particle vaccine against foot-and-mouth disease

文献类型： 外文期刊

第一作者： Chen, Liang

作者： Chen, Liang

作者机构：

关键词： Foot-and-mouth disease (FMD);Virus-like particles (VLP);Vaccine;Immunity

期刊名称：ANTIVIRAL RESEARCH （ 影响因子：5.97； 五年影响因子：5.801 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Foot-and-mouth disease (FMD) has caused severe economic losses to millions of farmers worldwide. In this work, the coding genes of 141-160 epitope peptide (EPi4i_i6o) of VP1 were inserted into the coat protein (CP) genes of MS2 in prokaryotic expression vector, and the recombinant protein self-assembled into virus-like particles (VLP). Results showed that the CP-EP141_160 VLP had a strong immunoreaction with the FMD virus (FMDV) antigen in vitro, and also had an effective immune response in mice. Further virus challenge tests were carried out on guinea pigs and swine, high-titer neutralizing antibodies were produced and the CP-EP14i_16o VLP vaccine could protect most of the animals against FMDV. Foot-and-mouth disease (FMD) has brought severe economic losses to stockbreeding and it continues to be a serious threat (Sobrino et al., 2001; Pengyan et al., 2008). Vaccination and slaughter of the infected or exposed herds are the main measures taken to control outbreaks of FMD. Conventional inactivated vaccine has played a leading role in controlling epidemics (Chen et al., 2006, 2004; Wang et al., 2002). However, it also presents the risk of virus escaping from production facilities (Strohmaier et al., 1982; Guo et al., 2005; King et al., 1981). Besides, it is hard to distinguish carrier animals from uninfected vaccinated animals (Wang et al., 2002). Moreover, its thermal instability, short protection period and other hazardous factors make the traditional vaccine far from optimal (Wang et al., 2002; Shao et al., 2011). Thus, it is urgent to develop alternative effective and safe vaccines. FMD virus (FMDV), the etiological agent of FMD, has seven distinguishable serological types and more than 70 subtypes (Domingo et al., 2002; Bachrach, 1968; Knowles and Samuel, 2003).

分类号： R37

  • 相关文献

[1]Comparative study of the immune effect of an Edwardsiella tarda antigen in two forms: Subunit vaccine vs DNA vaccine. Sun, Yun,Sun, Li,Sun, Yun,Liu, Chun-Sheng.

[2]ProtectiveimmunityinducedbyaDNAvaccineexpressingeIF4AofToxoplasmagondiiagainstacutetoxoplasmosisinmice. 陈佳,SiyangHuang,ZhongyuanLi,ZiguoYuan,DonghuiZhou,EskildPetersen,NianzhangZhang,XingquanZhu. 2013

[3]Schistosoma japonicum: Cloning, expression and characterization of a gene encoding the alpha 5-subunit of the proteasome. Hong, Yang,Han, Hongxiao,Peng, Jinbiao,Li, Ye,Shi, Yaojun,Fu, Zhiqiang,Liu, Jinming,Lin, Jiaojiao,Hong, Yang,Han, Hongxiao,Li, Xiangrui. 2010

[4]Expression of an immunologically (MSP-1(42)) reactive merozoite surface protein in E-coli. Leung, WH,Meng, ZQ,Hui, G,Ho, WK.

[5]A multivalent killed whole-cell vaccine induces effective protection against Edwardsiella tarda and Vibrio anguillarum. Sun, Yun,Sun, Li,Liu, Chun-sheng,Sun, Yun.

[6]Foot-and-mouth disease vaccines: progress and problems. Cao, Yimei,Lu, Zengjun,Liu, Zaixin.

[7]Studying the Mechanism of Plasmopara viticola RxLR Effectors on Suppressing Plant Immunity. Li, Xinlong,Wu, Jiao,Zhang, Yali,Lu, Jiang,Yin, Ling. 2016

[8]Transgenic Eimeria magna Perard, 1925 Displays similar Parasitological Properties to the Wild-type strain and induces an exogenous Protein-specific immune response in rabbits (Oryctolagus cuniculus L.). Tao, Geru,Tang, Xinming,Wang, Yunzhou,Li, Chao,Suo, Jingxia,Tian, Xiuling,Liu, Xianyong,Suo, Xun,Tao, Geru,Tang, Xinming,Wang, Yunzhou,Li, Chao,Suo, Jingxia,Tian, Xiuling,Liu, Xianyong,Suo, Xun,Tao, Geru,Tang, Xinming,Wang, Yunzhou,Li, Chao,Suo, Jingxia,Tian, Xiuling,Liu, Xianyong,Suo, Xun,Shi, Tuanyuan,Duszynski, Donald W.. 2017

[9]Fasted and postprandial response of serum physiological response, hepatic antioxidant abilities and HSP70 expression in Wuchang bream (Megalobrama amblycephala) fed different dietary carbohydrate levels. Zhou, Chuanpeng,Ge, Xianping,Liu, Bo,Xie, Jun,Chen, Ruli,Zhou, Chuanpeng. 2014

[10]Effect of High Dietary Carbohydrate on Growth, Serum Physiological Response, and Hepatic Heat Shock Protein 70 Expression of Wuchang Bream (Megalobrama amblycephala) at Two Temperatures. Zhou, Chuanpeng,Liu, Bo,Xie, Jun,Ge, Xianping,Liu, Bo,Xie, Jun,Ge, Xianping. 2013

[11]Transcriptome analysis of the spleen of the darkbarbel catfish Pelteobagrus vachellii in response to Aeromonas hydrophila infection. Wen, Zhengyong,Yuan, Dengyue,Shao, Ting,Wang, Jun,Li, Huatao,Gong, Quan. 2017

[12]Screening and analysis of PoAkirin1 and two related genes in response to immunological stimulants in the Japanese flounder (Paralichthys olivaceus). Yang, Chang-Geng,Sun, Bing,Liu, Shan-Shan,Chen, Song-Lin,Yang, Chang-Geng,Wang, Xian-Li,Zhang, Bo. 2013

[13]Identification and screening of effective protective antigens for channel catfish against Streptococcus iniae. Wang, Yajun,Wang, Erlong,He, Yang,Wang, Kaiyu,Yang, Qian,Wang, Jun,Geng, Yi,Ouyang, Ping,Lai, Weimin,Wang, Yajun,Wang, Kaiyu,Geng, Yi,Chen, Defang,Huang, Xiaoli. 2017

[14]Molecular characterization and expression analysis of a novel r-spondin member (rspo2l) in Chinese tongue sole (Cynoglossus semilaevis). Wei, Min,Zhao, Fa-zhen,Chen, Song-lin,Wei, Min,Xu, Wen-teng,Wang, Lei,Xiu, Yun-ji,Yang, Ying-ming,Li, Yang-zhen,Zhao, Fa-zhen,Chen, Song-lin,Wei, Min,Xu, Wen-teng,Wang, Lei,Xiu, Yun-ji,Yang, Ying-ming,Li, Yang-zhen,Zhao, Fa-zhen,Chen, Song-lin,Li, Hai-long. 2018

[15]Multiplex immune-related genes expression analysis response to bacterial challenge in mud crab, Scylla paramamosain. Zhang, Fengying,Jiang, Keji,Sun, Manman,Zhang, Dan,Ma, Lingbo. 2013

[16]The warm temperature acclimation protein Wap65 as an immune response gene: Its duplicates are differentially regulated by temperature and bacterial infections. Sha, Zhenxia,Yu, Peng,Takano, Tomokazu,Liu, Hong,Terhune, Jeffrey,Liu, Zhanjiang,Sha, Zhenxia,Liu, Hong. 2008

[17]Protochordate amphioxus is an emerging model organism for comparative immunology. Zhang, Shicui,Liang, Yujun,Ji, Guangdong,Zhuang, Zhimeng. 2009

[18]Iron-metabolic function and potential antibacterial role of Hepcidin and its correlated genes (Ferroportin 1 and Transferrin Receptor) in Turbot (Scophthalmus maximus). Yang, Chang-Geng,Liu, Shan-Shan,Sun, Bing,Wang, Na,Chen, Song-Lin,Yang, Chang-Geng,Sun, Bing,Wang, Xian-Li. 2013

[19]Influence of Ligustrum lucidum and Schisandra chinensis fruits on antioxidative metabolism and immunological parameters of layer chicks. Ma, Deying,Liu, Yuqin,Liu, Shengwang,Li, Qundao,Shan, Anshan. 2007

[20]Differential physiological effects of neonicotinoid insecticides on honey bees: A comparison between Apis mellifera and Apis cerana. Li, Zhiguo,Li, Meng,He, Jingfang,Zhao, Xiaomeng,Huang, Wei-Fone,Nie, Hongyi,Zhao, Yazhou,Su, Songkun,Chaimanee, Veeranan,Zhao, Yazhou. 2017

作者其他论文 更多>>

微信小程序