Molecular characterization and mRNA expression of hypoxia inducible factor-1 and cognate inhibiting factor in Macrobrachium nipponense in response to hypoxia

文献类型： 外文期刊

第一作者： Sun, Shengming

作者： Sun, Shengming;Fu, Hongtuo;Ge, Xianping;Zhu, Jian;Qiao, Hui;Jin, Shubo;Zhang, Wenyi;Xuan, Fujun

作者机构：

关键词： Hypoxia;HIF-1;FIH-1;Gene expression;Macrobrachium nipponense

期刊名称：COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY （ 影响因子：2.231； 五年影响因子：2.215 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Hypoxia inducible factors (HIFs) are considered to be the master switches of oxygen-dependent gene expression in mammalian species. Currently, very little is known about the function of this important pathway or the molecular structures of key players in the hypoxia-sensitive Oriental River Prawn Macro brachium nipponense. In this study, HIFs-1 alpha (HIF-1 alpha),-1 beta (HIF-1 beta) and HIF 1-alpha inhibitor (FIH-1) from M. nipponense were cloned. The 4903-bp cDNA of M. nipponense HIF-1 alpha (MnHIF-1 alpha) encodes a protein of 1088 aa, M. nipponense HIF-1 beta (MnHIF-1 beta) spans 2042 bp encoding 663 aa and the 1163 bp M. nipponense NH-1 (MnFIH-1) specifies a polypeptide of 345 aa. MnHIF-1 and MnFIH-1 homologs exhibit significant sequence similarity and share key functional domains with previously described vertebrate and invertebrate isoforms. Phylogenetic analysis identifies that genetic diversification of HIF-1 and FIH-1 occurred within the invertebrate lineage, indicating functional specialization of the oxygen sensing pathways in this group. Quantitative real-time RT-PCR demonstrated that MnHIF-1 and MnFIH-1 mRNA are expressed in different tissues and exhibit transcriptional responses to severe hypoxia in gill and muscle tissue, consistent with their putative role in oxygen sensing and the adaptive response to hypoxia. The role of HIF-1 alpha in response to hypoxia was further investigated in the gills and muscles of prawns using in situ hybridization. These results suggested that HIF-1 alpha plays an important role in oxygen sensing and homeostasis in M. nipponense. (C) 2016 Elsevier Inc. All rights reserved.

分类号： Q5`Q6

  • 相关文献

[1]Molecular Cloning and Functional Characterization of a Hexokinase from the Oriental River Prawn Macrobrachium nipponense in Response to Hypoxia. Sun, Shengming,Fu, Hongtuo,Zhu, Jian,Ge, Xianping,Xuan, Fujun. 2017

[2]Transciptomic and histological analysis of hepatopancreas, muscle and gill tissues of oriental river prawn (Macrobrachium nipponense) in response to chronic hypoxia. Sun, Shengming,Fu, Hongtuo,Zhu, Jian,Ge, Xianping,Xuan, Fujun,Gu, Zhimin. 2015

[3]Identification of differentially expressed genes in hepatopancreas of oriental river prawn, Macrobrachium nipponense exposed to environmental hypoxia. Sun, Shengming,Ge, Xianping,Fu, Hongtuo,Zhu, Jian,Xuan, Fujun,Zhang, Shiyong.

[4]Identification and comparative analysis of the oriental river prawn (Macrobrachium nipponense) microRNA expression profile during hypoxia using a deep sequencing approach. Sun, Shengming,Fu, Hongtuo,Ge, Xianping,Zhu, Jian,Gu, Zhimin,Xuan, Fujun.

[5]Comparative proteomic study of the response to hypoxia in the muscle of oriental river prawn (Macrobrachium nipponense). Sun, Shengming,Fu, Hongtuo,Ge, Xianping,Zhu, Jian,Qiao, Hui,Jin, Shubo,Zhang, Yiwen,Xuan, Fujun.

[6]Molecular cloning, characterization, and expression analysis of p53 from the oriental river prawn, Macrobrachium nipponense, in response to hypoxia. Sun, Shengming,Fu, Hongtuo,Zhu, Jian,Ge, Xianping,Gu, Zhimin,Xuan, Fujun.

[7]THE METALLOTHIONEIN GENE FROM THE ORIENTAL RIVER PRAWN MACROBRACHIUM NIPPONENSE (DE HAAN, 1849): CHARACTERIZATION AND EXPRESSION IN RESPONSE TO HYPDXIA AND REOXYGENATION. Sun, Shengming,Gu, Zhimin,Fu, Hongtuo,Zhu, Jian.

[8]Global gene expression responses to waterlogging in roots of sesame (Sesamum indicum L.). Wang, Linhai,Zhang, Yanxin,Qi, Xiaoqiong,Li, Donghua,Wei, Wenliang,Zhang, Xiurong. 2012

[9]Identification and characterization of a Macrobrachium nipponense ferritin subunit regulated by iron ion and pathogen challenge. Sun, Shengming,Gu, Zhimin,Gu, Zhimin,Sun, Shengming,Fu, Hongtuo,Zhu, Jian,Ge, Xianping,Xuan, Fujun.

[10]Molecular cloning and expression analysis of female sterile homeotic gene (fsh) in the oriental river prawn Macrobrachium nipponense. Zhang, W. Y.,Fu, H. T.,Zhang, W. Y.,Jiang, S. F.,Xiong, Y. W.,Fu, H. T.,Qiao, H.,Sun, S. M.,Gong, Y. S.,Jin, S. B.. 2015

[11]The specific expression pattern of globin mRNAs in Tibetan chicken during late embryonic stage under hypoxia. Liu, C.,Zhang, L. F.,Li, N.. 2013

[12]Dietary wolfberry upregulates carotenoid metabolic genes and enhances mitochondrial biogenesis in the retina of db/db diabetic mice. Yu, Huifeng,Wark, Logan,Ji, Hua,Jaing, Yu,Han, Jing,Ortiz, Edlin,Zhang, Yunong,Medeiros, Denis M.,Lin, Dingbo,Ji, Hua,He, Hui,Willard, Lloyd,Medeiros, Denis M.. 2013

[13]Editorial: Eutrophication and hypoxia and their impacts on the ecosystem of the Changjiang Estuary and adjacent coastal environment. Zhang, Jing,Xiao, Tian,Huang, Daji,Liu, Su Mei,Fang, Jianguang. 2016

[14]Respiratory response of grass carp Ctenopharyngodon idellus to dissolved oxygen changes at three acclimation temperatures. Zhao, Zhigang,Xu, Qiyou,Dong, Shuanglin. 2018

[15]Comparative analysis of sequence feature and expression of two heat shock cognate 70 genes in mandarin fish Siniperca chuatsi. Wang, Pengfei,Xu, Peng,Zeng, Shuang,Zhou, Lei,Zeng, Lei,Li, Guifeng,Wang, Pengfei,Xu, Peng,Zeng, Shuang,Zhou, Lei,Zeng, Lei,Li, Guifeng,Wang, Pengfei.

[16]Flaxseed Oil Alleviates Chronic HFD-Induced Insulin Resistance through Remodeling Lipid Homeostasis in Obese Adipose Tissue. Yu, Xiao,Shen, Ruiling,Yu, Xiao,Shen, Ruiling,Tang, Yuhan,Liu, Peiyi,Xiao, Lin,Liu, Liegang,Yao, Ping,Tang, Yuhan,Liu, Peiyi,Xiao, Lin,Liu, Liegang,Yao, Ping,Deng, Qianchun,Deng, Qianchun,Deng, Qianchun.

[17]Duplication and differentiation of common carp (Cyprinus carpio) myoglobin genes revealed by BAC analysis. Zhao, Zi-Xia,Xu, Peng,Deng, Hai-Xia,Zhang, Yan,Xu, Li-Ming,Li, Jiong-Tang,Xu, Jian,Sun, Xiao-Wen,Cao, Ding-Chen,Kuang, You-Yi,Sun, Xiao-Wen.

[18]Studies on resistance characteristic and cDNA sequence conservation of transferrin from crucian carp, Carassius auratus. Long, Hua,Yu, Qi-Xing.

[19]Bacterial growth efficiency in a partly eutrophicated bay of South China Sea: Implication for anthropogenic impacts and potential hypoxia events. Song, Xing-Yu,Liu, Hua-Xue,Zhong, Yu,Tan, Ye-Hui,Qinl, Geng,Li, Kai-Zhi,Shen, Ping-Ping,Huang, Liang-Min,Wang, You-Shao,Song, Xing-Yu,Zhong, Yu,Wang, You-Shao,Liu, Hua-Xue.

[20]Diversification of the duplicated Rab1a genes in a hypoxia-tolerant fish, common carp (Cyprinus carpio). Zhao, Zi-Xia,Xu, Jian,Xu, Ru,Li, Jiong-Tang,Zhang, Yan,Xu, Peng,Sun, Xiao-Wen,Cao, Ding-Chen,Sun, Xiao-Wen.

作者其他论文 更多>>

微信小程序