Construction of the BAC Library of Small Abalone (Haliotis diversicolor) for Gene Screening and Genome Characterization
文献类型: 外文期刊
第一作者: Jiang, Likun
作者: Jiang, Likun;Xu, Jian;Jiang, Yanliang;Wang, Kai;Zhao, Zixia;Chen, Baohua;Zhao, Yunfeng;Xu, Peng;Jiang, Likun;Xu, Jian;Jiang, Yanliang;Wang, Kai;Zhao, Zixia;Chen, Baohua;Zhao, Yunfeng;Xu, Peng;You, Weiwei;Ke, Caihuan;Zhang, Xiaojun;Jiang, Likun;Chen, Baohua;Mahboob, Shahid;Al-Ghanim, Khalid A.;Mahboob, Shahid
作者机构:
关键词: BAC;Small abalone;Haliotis diversicolor;Genome;Ion Torrent
期刊名称:MARINE BIOTECHNOLOGY ( 影响因子:3.619; 五年影响因子:3.322 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: The small abalone (Haliotis diversicolor) is one of the most important aquaculture species in East Asia. To facilitate gene cloning and characterization, genome analysis, and genetic breeding of it, we constructed a large-insert bacterial artificial chromosome (BAC) library, which is an important genetic tool for advanced genetics and genomics research. The small abalone BAC library includes 92,610 clones with an average insert size of 120 Kb, equivalent to approximately 7.6x of the small abalone genome. We set up three-dimensional pools and super pools of 18,432 BAC clones for target gene screening using PCR method. To assess the approach, we screened 12 target genes in these 18,432 BAC clones and identified 16 positive BAC clones. Eight positive BAC clones were then sequenced and assembled with the next generation sequencing platform. The assembled contigs representing these 8 BAC clones spanned 928 Kb of the small abalone genome, providing the first batch of genome sequences for genome evaluation and characterization. The average GC content of small abalone genome was estimated as 40.33 %. A total of 21 protein-coding genes, including 7 target genes, were annotated into the 8 BACs, which proved the feasibility of PCR screening approach with three-dimensional pools in small abalone BAC library. One hundred fifty microsatellite loci were also identified from the sequences for marker development in the future. The BAC library and clone pools provided valuable resources and tools for genetic breeding and conservation of H. diversicolor.
分类号: Q81
- 相关文献
作者其他论文 更多>>
-
Effects of Substrate Composition on the Growth Traits of Grafted Seedling in Macadamia (Macadamia integrifolia) Nuts
作者:Tan, Qiujin;Zhou, Chunheng;Xu, Peng;Huang, Xiyun;Pan, Zhenzhen;Wei, Yuanrong;Wang, Wenlin;Wang, Lifeng;Wang, Lifeng
关键词:grafted seedling; growth; Macadamia integrifolia; substrate; surviving rate
-
An analysis of lncRNAs related to fiber quality and the discovery of their target genes in a Gossypium hirsutum line with Gossypium mustelinum introgression
作者:Wang, Baohua;Ji, Meijun;Fang, Hui;Gu, Haijing;Mehari, Teame Gereziher;Han, Jinlei;Feng, Wenxiang;Wang, Kai;Huo, Xuehan;Zhang, Jingxia;Chen, Yu;Zhang, Jun;Ditta, Allah;Khan, Muhammad K. R.;Paterson, Andrew H.;Chee, Peng W.
关键词:
-
Exploring the Therapeutic Potential of Royal Jelly in Metabolic Disorders and Gastrointestinal Diseases
作者:El-Seedi, Hesham R.;El-Seedi, Hesham R.;Guo, Zhiming;El-Seedi, Hesham R.;Salama, Suzy;El-Wahed, Aida A. Abd;Di Minno, Alessandro;Daglia, Maria;Buccato, Daniele Giuseppe;Di Minno, Alessandro;Daglia, Maria;Li, Chuan;Guan, Xiao;Guan, Xiao;Khalifa, Shaden A. M.;Wang, Kai
关键词:royal jelly; diabetes mellitus; gastrointestinal diseases; cardiovascular diseases; bioactive compounds
-
Spatiotemporal response of ecosystem services to tourism activities in urban forests
作者:Li, Jiadan;Zhang, Xian;Wang, Kai;Xu, Zhihao;Gu, Qing;Zhang, Zhongchu
关键词:ecosystem service value; land use and land cover; tourism activities; geographically weighted regression; internet big data; tourism management strategies; urban forest
-
New Insights into Identification, Distribution, and Health Benefits of Polyamines and Their Derivatives
作者:Qiao, Jiangtao;Cai, Wenwen;Wang, Kai;Dong, Jie;Xu, Xiang;Qiao, Jiangtao;Wang, Kai;Haubruge, Eric;Cai, Wenwen;Dong, Jie;Zhang, Hongcheng;El-Seedi, Hesham R.;El-Seedi, Hesham R.;El-Seedi, Hesham R.
关键词:conjugated polyamines; spermidine; functionalproperties; nutraceuticals
-
Dietary Flavonoid Quercetin Supplement Promotes Antiviral Innate Responses Against Vesicular Stomatitis Virus Infection by Reshaping the Bacteriome and Host Metabolome in Mice
作者:Li, Tingting;Zhu, Jinfeng;Zhu, Yinrui;Pei, Pei;Yang, Kai;Hu, Lin;Li, Tingting;Zhu, Jinfeng;Zhu, Yinrui;Pei, Pei;Yang, Kai;Hu, Lin;Li, Tingting;Wu, Chao;Chen, Nannan;Zhu, Jinfeng;Yu, Qifeng;Zheng, Xing;Wang, Kai
关键词:gut microbiota; metabolomics; pro-inflammatory cytokines; quercetin; vesicular stomatitis virus
-
OsCOL5 suppresses heading through modulation of Ghd7 and Ehd2, enhancing rice yield
作者:Wen, Xiaoxia;Zhong, Zhengzheng;Xu, Peng;Yang, Qinqin;Liu, Ling;Wu, Zhaozhong;Wu, Yewen;Zhang, Yingxin;Liu, Qunen;Zhou, Zhengping;Peng, Zequn;Cheng, Shihua;Cao, Liyong;Zhan, Xiaodeng;Wu, Weixun;Wen, Xiaoxia;Zhong, Zhengzheng;Xu, Peng;Yang, Qinqin;Liu, Ling;Wu, Zhaozhong;Wu, Yewen;Zhang, Yingxin;Liu, Qunen;Zhou, Zhengping;Peng, Zequn;Cheng, Shihua;Cao, Liyong;Zhan, Xiaodeng;Wu, Weixun;Wen, Xiaoxia;Liu, Ling;He, Yuqing;Wang, Yinping;Wang, Yinping
关键词: