Comparative transcriptome analyses indicate enhanced cellular protection against FMDV in PK15 cells pretreated with IFN-gamma

文献类型： 外文期刊

第一作者： Fu, Yin

作者： Fu, Yin;Zhu, Zesen;Liu, Jing;Chen, Huiyong;Fu, Yin;Zhu, Zesen;Liu, Jing;Chen, Huiyong;Chang, Huiyun;Liu, Zaixin

作者机构：

关键词： RNA sequencing;Differently expressed genes;Apoptosis;Immune response

期刊名称：GENE （ 影响因子：3.688； 五年影响因子：3.329 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： Interferon gamma (IFN-gamma) can induce a host antiviral response to foot and mouth disease virus (FMDV) in vivo and in vitro. To elucidate the mechanism of IFN-gamma anti FMDV infection in host cells, high-throughput RNA sequencing was analyzed for systemic changes in gene expression profiles in PK15 cells infected by FMDV with or without IFN-gamma pretreatment. More than 25 million reads, covering 1.2-1.5 Gb, were analyzed from each experiment panel. FMDV challenge altered the transcription of genes involved in positively and negatively regulating cell death or apoptosis; however, the expected immune suppression response was not obvious. IFN-gamma pretreatment combined with FMDV infection normalized the increase in apoptosis. Furthermore, the transcription factors required for IFN-gamma functioning, STAT1 and IRF1 were up-regulated by IFN-gamma pretreatment and stimulated downstream IFN-stimulated genes (ISGs). These induced ISGs are mainly responsible for antigen processing, antigen presentation or antiviral defense. Interestingly, a synergistic effect on some ISGs, including OAS1, OAS2, MX1, MX2, RIG-I and IFIT1, was observed in the combined treatment compared to the IFN-gamma treatment alone. The suggested effects identified by RNA sequencing were consistent with cellular morphology changes and confirmed by related protein markers. This is the first report exploring transcriptome alterations introduced by FMDV infection with or without IFN-gamma pretreatment. The identified key host genes that control cell survival in vitro broaden our comprehensive understanding of how IFN-gamma inhibits FMDV infection and may shed light on developing improved FMD control approaches. (C) 2016 Elsevier B.V. All rights reserved.

分类号： R394

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