Identification of piroplasm infection in questing ticks by RLB: a broad range extension of tick-borne piroplasmin China?

文献类型： 外文期刊

第一作者： Abdallah, Mirza Omar

作者： Abdallah, Mirza Omar;Niu, Qingli;Yu, Peifa;Guan, Guiquan;Yang, Jifei;Chen, Ze;Liu, Guangyuan;Luo, Jianxun;Yin, Hong;Yin, Hong;Wei, Yonghong

作者机构：

关键词： Piroplasm;Reverse line blot;Detection;Differentiation

期刊名称：PARASITOLOGY RESEARCH （ 影响因子：2.289； 五年影响因子：2.403 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Sensitive and specific diagnostic method for rapid and simultaneous detection and discrimination of the different species is needed for an effective control of piroplasmosis. Here, a reverse line blot (RLB) assay was developed for piroplasm detection. A general pair of primer based on 18S ribosomal RNA (rRNA) gene was used to amplify V4 region of 18S rRNA gene. General and specific probes for 13 piroplasm species were cited from previous publications or designed according to the alignment of 18S rRNA gene sequences. For sensitivity test of RLB assay, serially diluted plasmids of the different species were used to access the sensitivity of the RLB. Four hundred and fifty tick samples collected from grass from different provinces of China were then detected. The result indicated that the RLB assay is highly specific and sensitive, detecting up to 10(2) copies/mu l of recombinant plasmid DNA. Multiple piroplasms were detected as single or mixed infection from tick species. Eight piroplasm species, most of which were Theileria annulata (33/450, 7.3 %) or Babesia sp. Xinjiang (30/450, 6.7 %), were found to infect with 89 tick samples in four tick species; no infections with Babesia major, Babesia ovata, Babesia bigemina, Theileria sergenti, or Theileria equi were detected. The piroplasms species-specific RLB assay may have potential clinical application in the simultaneous detection and differentiation of Babesia and Theileria species.

分类号： R53

  • 相关文献

[1]First Molecular Detection of Piroplasm Infection in Pet Dogs from Gansu, China. Niu, Qingli,Yang, Jifei,Liu, Zhijie,Gao, Shandian,Pan, Yuping,Guan, Guiquan,Chu, Yuefeng,Liu, Guangyuan,Luo, Jianxun,Yin, Hong,Yin, Hong. 2017

[2]Detection and differentiation of influenza viruses with glycan-functionalized gold nanoparticles. Zheng, Longtang,Lv, Xun,Bi, Yuhai,Zhang, Zhenxing,Wang, Pengfei,Liu, Ruichen,Jiang, Jingwen,Cong, Haolong,Liu, Wenjun,Gao, George F.,Li, Xuebing,Li, Xuebing,Zheng, Longtang,Zhang, Zhenxing,Wang, Pengfei,Jiang, Jingwen,Li, Xuebing,Wei, Jinhua,Du, Yuguang,Wu, Peixing,Liang, Jingnan,Cheng, Wenwen,Jiang, Xingyu,Cao, Hongzhi,Bi, Yuhai,Liu, Wenjun,Gao, George F.,Li, Xuebing,Cheng, Wenwen.

[3]Loop-mediated isothermal amplification (LAMP) method based on two species-specific primer sets for the rapid identification of Chinese Babesia bovis and B. bigemina. Liu, Aihong,Guan, Guiquan,Du, Pengfei,Gou, Huitian,Liu, Zhijie,Liu, Junlong,Ma, Milin,Yang, Jifei,Li, Youquan,Niu, Qinli,Ren, Qiaoyun,Bai, Qi,Yin, Hong,Luo, Jianxun.

[4]Detecting and differentiating Theileria sergenti and Theileria sinensis in cattle and yaks by PCR based on major piroplasm surface protein (MPSP). Liu, Aihong,Guan, Guiquan,Liu, Zhijie,Liu, Junlong,Li, Youquan,Gao, Jinliang,Ma, Milin,Niu, Qinli,Ren, Qiaoyun,Bai, Qi,Yin, Hong,Luo, Jianxun,Leblanc, Neil. 2010

[5]Genetic variability assessed by microsatellites in the breeding populations of the shrimp Penaeus (Fenneropenaeus) chinensis in China. Zhang, Tianshi,Kong, Jie,Wang, Weiji,Wang, Qingyin,Zhang, Tianshi.

[6]Establishment of a pluripotent embryonic cell line from sea perch (Lateolabrax japonicus) embryos. Cher, SL,Sha, ZX,Ye, HQ.

[7]MicroRNA-95 promotes myogenic differentiation by down-regulation of aminoacyl-tRNA synthase complex-interacting multifunctional protein 2. Li, Biao,Xie, Shanshan,Cai, Chunbo,Qian, Lili,Jiang, Shengwang,Ma, Dezun,Xiao, Gaojun,Gao, Ting,Cui, Wentao,Yang, Jinzeng. 2017

[8]Genetic diversity and differentiation of Camellia sinensis L. (cultivated tea) and its wild relatives in Yunnan province of China, revealed by morphology, biochemistry and allozyme studies. Jin, C,Wang, PS,Xia, YM,Xu, M,Pei, SJ. 2005

[9]Isolation and Characterization of Mesenchymal Stem Cells Derived from Duck Embryo Liver. Wang, Wenjie,Guan, Weijun,Ma, Yuehui,Huang, Hongmei,Li, Qian,Wang, KunFu,Chen, Jia. 2017

[10]Klf7 modulates the differentiation and proliferation of chicken preadipocyte. Zhang, Zhiwei,Wang, Haixia,Sun, Yingning,Li, Hui,Wang, Ning,Zhang, Zhiwei,Wang, Haixia,Sun, Yingning,Li, Hui,Wang, Ning,Zhang, Zhiwei,Wang, Haixia,Sun, Yingning,Li, Hui,Wang, Ning,Sun, Yingning. 2013

[11]Direct and indirect shoot and bulblet regeneration from cultured leaf explants of Lilium pumilum, an endangered species. Jin, Shumei,Wang, Ji,Sun, Dan,Liu, Shengkui,Wang, Xinwang,Genovesi, A. D.,Li, Guoliang. 2014

[12]Cocktail supplement with rosiglitazone: a novel inducer for chicken preadipocyte differentiation in vitro. Cheng, Bohan,Wu, Mengqi,Xu, Songsong,Zhang, Xinyang,Wang, Yuxiang,Wang, Ning,Leng, Li,Li, Hui,Cheng, Bohan,Wu, Mengqi,Xu, Songsong,Zhang, Xinyang,Wang, Yuxiang,Wang, Ning,Leng, Li,Li, Hui,Cheng, Bohan,Wu, Mengqi,Xu, Songsong,Zhang, Xinyang,Wang, Yuxiang,Wang, Ning,Leng, Li,Li, Hui. 2016

[13]IGF1 regulates RUNX1 expression via IRS1/2: Implications for antler chondrocyte differentiation. Yang, Zhan-Qing,Zhang, Hong-Liang,Geng, Shuang,Wang, Kai,Yu, Hai-Fan,Yue, Zhan-Peng,Guo, Bin,Duan, Cui-Cui. 2017

[14]Oleate promotes differentiation of chicken primary preadipocytes in vitro. Shang, Zhouchun,Guo, Lin,Wang, Ning,Shi, Hui,Wang, Yuxiang,Li, Hui,Shang, Zhouchun,Guo, Lin,Wang, Ning,Shi, Hui,Wang, Yuxiang,Li, Hui,Shang, Zhouchun,Guo, Lin,Wang, Ning,Shi, Hui,Wang, Yuxiang,Li, Hui. 2014

[15]Immortalized bovine mammary epithelial cells express stem cell markers and differentiate in vitro. Hu, Han,Zheng, Nan,Hu, Han,Gao, Haina,Dai, Wenting,Zhang, Yangdong,Li, Songli,Wang, Jiaqi.

[16]Seed coat development and its evolutionary implications in diploid and amphidiploid Brassica species. Zeng, Chang-Li,Wu, Xiao-Ming,Wang, Jian-Bo.

[17]Genetic differentiation in seven geographic populations of the fleshy shrimp Penaeus (Fenneropenaeus) chinensis based on microsatellite DNA. Meng, Xian Hong,Wang, Qing Yin,Liu, Ping,Kong, Jie,Meng, Xian Hong,Jang, In Kwon.

[18]Duplication and differentiation of common carp (Cyprinus carpio) myoglobin genes revealed by BAC analysis. Zhao, Zi-Xia,Xu, Peng,Deng, Hai-Xia,Zhang, Yan,Xu, Li-Ming,Li, Jiong-Tang,Xu, Jian,Sun, Xiao-Wen,Cao, Ding-Chen,Kuang, You-Yi,Sun, Xiao-Wen.

[19]Subcellular localization, expression patterns, SNPs and association analyses of the porcine HUMMLC2B gene. Wang, Huan L.,Wang, Heng,Zhu, Zheng M.,Wang, Chen F.,Zhu, Meng J.,Mo, De L.,Yang, Shu L.,Li, Kui.

[20]Use of mitochondrial RNA genes for the differentiation of four Trichinella species by multiplex PCR amplification. Blaga, R.,Fu, BaoQuan,Le Rhun, D.,Le Naour, E.,Heckman, A.,Zocevic, A.,Boireau, P.,Liu, MingYuan.

作者其他论文 更多>>

微信小程序