Simultaneous determination of 14 antiviral drugs and relevant metabolites in chicken muscle by UPLC-MS/MS after QuEChERS preparation

文献类型: 外文期刊

第一作者: Qian, Yongzhong

作者: Qian, Yongzhong;Qiu, Jing

作者机构:

关键词: Antiviral drugs;Metabolites;UPLC-MS/MS;QuEChERS method;Chicken muscle

期刊名称:JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES ( 影响因子:3.205; 五年影响因子:3.068 )

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收录情况: SCI

摘要: A fast method for the simultaneous determination of 14 antiviral drugs and relevant metabolites in chicken muscle by ultra-high "liquid chromatography tandem mass spectrometry (UPLC MS/MS) was developed. The analytes included anti-influenza drugs (amantadine, rimantadine, oseltamivir, oseltamivir carboxylate, memantine, arbidol, and moroxydine), anti-herpes drugs (acyclovir, ganciclovir, famciclovir, penciclovir, ribavirin and its main metabolite TCONH2), and an immunomodulator (imiquimod). The samples were pretreated using a modified QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) method. The determination of the target compounds was conducted in less than 11.0 min, and specificity was ensured by the use of multiple reaction monitoring (MRM) acquisition mode. Good linearities (R-2 > 0.9928) were obtained in the range of 0.1-100 pg/L, and the recovery rates were 56.2-113.4% with RSD5 of 1.7-10.3% for intra-day precision and 2.4-8.8% for inter-day precision. The LODs and LOQs of all analytes were in the ranges of 0.02-1.0 and 0.05-2.5 mu g/kg, respectively. An analysis of real samples suggested that this method is simple, sensitive, reliable and practical for the detection of antiviral drugs in animal tissue. (C) 2016 Elsevier B.V. All rights reserved.

分类号: Q503

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