Box-Behnken design for extraction optimization, characterization and in vitro antioxidant activity of Cicer arietinum L. hull polysaccharides

文献类型： 外文期刊

第一作者： Ye, Zipeng

作者： Ye, Zipeng;Yuan, Qingxia;Ye, Hong;Sun, Yi;Zeng, Xiaoxiong;Wang, Wei;Zhang, Hongcheng

作者机构：

关键词： Chickpea hull;Cicer arietinum L;Polysaccharide extraction;Box-Behnken design;In vitro antioxidant activity

期刊名称：CARBOHYDRATE POLYMERS （ 影响因子：9.381； 五年影响因子：8.678 ）

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年卷期：

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收录情况： SCI

摘要： The optimal extraction conditions with a yield of 5.37 +/- 0.15% for extraction of polysaccharides from chickpea (Cicer arietinum L.) hull (CHPS) were determined as extraction temperature 99 degrees C, extraction time 2.8 h and ratio of water to raw material 24 mL/g. Three fractions of CHPS-1, CHPS-2 and CHPS-3, with average molecular weight of 3.1 x 10(6), 1.5 x 10(6) and 7.8 x 10(5) Da, respectively, were obtained from crude CHPS by chromatography of DEAE Fast Flow and Sephadex G-100. CHPS-1 was composed of man nose, rhamnose, galactose, galacturonic acid, glucose and arabinose, CHPS-2 was composed of mannose, rhamnose, galacturonic acid, galactose, xylose and arabinose, CHPS-3 was composed of galacturonic acid, galactose and rhamnose. CHPS-3 showed the strongest reducing power and protective effect on H2O2-induced oxidative injury in PC12 cells and highest scavenging activities against DPPH and ABTS radicals, while CHPS-2 showed the highest scavenging activity against superoxide anion radical. (C) 2016 Elsevier Ltd. All rights reserved.

分类号： O629`Q53

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