Molecular characterization of Sp110 gene in pigs

文献类型： 外文期刊

第一作者： Li, Li-zu

作者： Li, Li-zu;Wang, Qiu-shi;Han, Li-xin;Wang, Jin-kui;Shao, Si-yu;Yang, Xiu-qin;Wang, Liang;Liu, Di

作者机构：

关键词： Pig;Sp110;Alternative splicing;Subcellular localization;Expression

期刊名称：MOLECULAR GENETICS AND GENOMICS （ 影响因子：3.291； 五年影响因子：3.257 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Speckled 110 kDa (Sp110) plays an important role in infectious diseases, as revealed by studies in humans. However, little is known regarding porcine Sp110. To elucidate its potential role in porcine resistance to viral diseases, here, the complete coding sequence of porcine Sp110 gene and its 26 alternatively spliced isoforms were isolated using reverse transcription (RT)-polymerase chain reaction (PCR), and another seven splicing patterns were obtained using a minigene construct. Subcellular distribution of 11 representative isoforms was characterized in PK-15 cells transiently transfected with their respective GFP fusion constructs, and only isoforms (R and V) bearing all functional domains were localized in nucleus, indicating all the other isoforms lose normal functions of Sp110 owing to alternative splicing. Real-time quantitative PCR and competitive RT-PCR showed that both isoforms R and V had similar tissue expression profile, half-life and response to poly(I:C), a synthetic analog of viral double-stranded RNA, while the longer one (isoform R) was transcribed at a higher level. The results indicated that porcine Sp110 has a role in viral infection and that isoform R is the dominant active form. Overall the data provide potential resource for molecular breeding of pig resistant to diseases and contributes to breeding pigs resistant to viral infection.

分类号： Q7

  • 相关文献

[1]Garlic virus X 11-kDa protein granules move within the cytoplasm and traffic a host protein normally found in the nucleolus. Lu, Yuwen,Yan, Fei,Zheng, Hongying,Lin, Lin,Peng, Jiejun,Chen, Jianping,Lu, Yuwen,Yan, Fei,Zheng, Hongying,Lin, Lin,Peng, Jiejun,Chen, Jianping,Guo, Wei,Peng, Jiejun.

[2]Subcellular localization, expression patterns, SNPs and association analyses of the porcine HUMMLC2B gene. Wang, Huan L.,Wang, Heng,Zhu, Zheng M.,Wang, Chen F.,Zhu, Meng J.,Mo, De L.,Yang, Shu L.,Li, Kui.

[3]Molecular Characterization of Tob1 in Muscle Development in Pigs. Yuan, Jing,Cao, Ji-Yue,Yuan, Jing,Tang, Zhong-Lin,Li, Kui,Wang, Ning. 2011

[4]Cloning and Characterization of New Splice Variants of Insulin-Like Growth Factor-1 Gene in Songliao Black Pig. Liu, Chang,Zhaoa, Zhihui,Bai, Wenlin,Sun, Boxing,Zhang, Yonghong,Yang, Runjun,Liu, Chang,Bai, Wenlin. 2012

[5]Alternative splicing of the antitrypsin gene in the silkworm, Bombyx mori. Liu, Hui-fen,Li, Yi-nue,Zhang, Zhi-fang,Liu, Hui-fen,Cui, Wei-zheng,Mu, Zhi-mei,Jia, Ru.

[6]Genome-wide characterization of the ankyrin repeats gene family under salt stress in soybean. Zhang, Dayong,Wan, Qun,He, Xiaolan,Ning, Lihua,Huang, Yihong,Xu, Zhaolong,Liu, Jia,Shao, Hongbo,Shao, Hongbo.

[7]Isolation and functional characterization of a Medicago sativa L. gene, MsLEA3-1. Sun, Yan,Sun, Yan,Bai, Yongqin,Yang, Qingchuan,Kang, Junmei,Chao, Yuehui,Gruber, Margaret.

[8]Molecular cloning and characterization of a novel gene encoding zinc finger protein from Medicago sativa L.. Chao, Yuehui,Kang, Junmei,Yang, Qingchuan,Wu, Mingsheng,Li, Yan,Chao, Yuehui,Wang, Pingqing,Long, Ruicai,Qin, Zhihui,Sun, Yan. 2009

[9]Molecular cloning and characterization of a group 3 LEA gene from Agropyron mongolicum Keng. Zhao, Yan,Yun, Jinfeng,Shi, Fengmin,Wang, Junjie,Yang, Qingchuan,Chao, Yuehui. 2010

[10]Localization, Expression Change in PRRSV Infection and Association Analysis of the Porcine TAP1 Gene. Sun, Nunu,Liu, Dewu,Meng, Fanming,Zhang, Xianwei,Wu, Zhenfang,Sun, Nunu,Liu, Xiangdong,Xie, Shengsong,Li, Xinyun,Sun, Nunu,Liu, Xiangdong,Xie, Shengsong,Li, Xinyun,Chen, Hongbo,Chen, Huiyong. 2012

[11]Molecular cloning, expression and variation analyses of the dopamine D2 receptor gene in pig breeds in China. Xu, H. P.,He, X. M.,Hu, Y. S.,Jia, X. Z.,Nie, Q. H.,Zhang, X. Q.,Xu, H. P.,He, X. M.,Hu, Y. S.,Jia, X. Z.,Nie, Q. H.,Zhang, X. Q.,Fang, M. X.. 2011

[12]Molecular characterization and chromosomal mapping of porcine brain and muscle Arnt-like protein-1 gene. Xing, Jinyi,Xu, Qinying,Jiang, Yunliang,Li, Kui,Wang, Jiying,Wu, Ying,Xing, Jinyi.

[13]Porcine granulin gene (GRN): Molecular cloning, polymorphism and chromosomal localization. Yang, Jine,Yu, Mei,Liu, Bang,Zhu, Mengjin,Li, Kui.

[14]Porcine methionine sulfoxide reductase B3: molecular cloning, tissue-specific expression profiles, and polymorphisms associated with ear size in Sus scrofa. Zhang, Yuebo,Liang, Jing,Zhang, Longchao,Wang, Ligang,Liu, Xin,Yan, Hua,Zhao, Kebin,Shi, Huibi,Zhang, Tian,Li, Na,Pu, Lei,Wang, Lixian,Li, Na. 2015

[15]Molecular characterization of porcine MMP19 and MMP23B genes and its association with immune traits. Zhao, Shuanping,Zhao, Yongzhen,Niu, Pengxia,Tang, Zhonglin,Li, Kui,Zhao, Shuanping,Zan, Linsen,Wang, Ning.

[16]Molecular cloning and expression of the porcine high-affinity immunoglobulin G Fc receptor (Fc gamma RI). Zhang, Gaiping,Qiao, Songlin,Li, Qingmei,Wang, Xuannian,Duan, Yanhua,Wang, Li,Xiao, Zhijun,Xia, Chun. 2006

[17]Characterization of miRNAs involved in response to poly(I: C) in porcine airway epithelial cells. Wang, L.,Wang, J. K.,Han, L. X.,Zhuo, J. S.,Du, X.,Yang, X. Q.,Wang, L.,Liu, D..

[18]Long non-coding RNA analysis of muscular responses to testosterone deficiency in Huainan male pigs. Xing, Baosong,Bai, Xianxiao,Guo, Hongxia,Chen, Junfeng,Hua, Liushuai,Zhang, Jiaqing,Ma, Qiang,Ren, Qiaoling,Wang, Jing,Wang, Huashuai.

[19]Molecular cloning and characterization of porcine interferon-induced protein with tetratricopeptide repeats (IFIT) 5. Zhang, Jiao,Shao, Si-yu,Li, Li-zu,Yang, Xiu-qin,Liu, Di.

[20]Renaturation and purification of ApxII toxin of Actinobacillus pleuropneumoniae. Wang, Chunlai,Liu, Siguo,Peng, Yonggang,Shao, Meili,Wang, Yong,Gong, Qiang,Chang, Yuehong,Liu, Jiandong,Liu, Huifang,Liu, Di,Kong, Xiangang.

作者其他论文 更多>>

微信小程序