Synergistic effects of ultraviolet-B and methyl jasmonate on tanshinone biosynthesis in Salvia miltiorrhiza hairy roots
文献类型: 外文期刊
第一作者: Wang, Cong Hui
作者: Wang, Cong Hui;Tian, Hao;Wang, Jian Wen;Zheng, Li Ping;Tian, Hao
作者机构:
关键词: Salvia miltiorrhiza;Hairy roots;Tanshinones;UV-B;MeJA;Elicitation
期刊名称:JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY ( 影响因子:6.252; 五年影响因子:5.375 )
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收录情况: SCI
摘要: Tanshinones are major bioactive diterpenoids of Salvia miltiorrhiza roots used for the treatment of cardiocerebral diseases. To develop effective elicitation and bioprocess strategies for the enhanced production of tanshinones, ultraviolet-B (UV-B) irradiation and methyl jasmonate (MeJA) elicitation were applied alone or in combination respectively in S. miltiorrhiza hairy root cultures. Our results showed 40-min UV-B irradiation at 40 mu W/cm(2) stimulated tanshinone production without any suppression of root growth, suggesting a new effective elicitor to S. miltiorrhiza hairy root cultures for tanshinone production. Moreover, the combined treatment of UV-B irradiation and MeJA exhibited synergistic effects on the expression levels of 3-hydroxy-3-methylglutrayl-CoA reductase (SmHMGR) and geranylgeranyl diphosphate synthase (SmGGPPS) genes in the tanshinone biosynthetic pathway. When hairy roots of 18-day-old cultures were exposed to the combined elicitation for 9 days, the maximum production of tanshinone reached to 28.21 mg/L, a 4.9-fold increase over the control. The combined elicitation of UV-B and MeJA was firstly used to stimulate the production of biologically important secondary metabolites in hairy root cultures. (C) 2016 Elsevier B.V. All rights reserved.
分类号: Q`O6
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