VdThit, a Thiamine Transport Protein, Is Required for Pathogenicity of the Vascular Pathogen Verticillium dahliae

文献类型： 外文期刊

第一作者： Qi, Xiliang

作者： Qi, Xiliang;Su, Xiaofeng;Guo, Huiming;Cheng, Hongmei;Qi, Xiliang;Qi, Juncang

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期刊名称：MOLECULAR PLANT-MICROBE INTERACTIONS （ 影响因子：4.171； 五年影响因子：4.836 ）

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收录情况： SCI

摘要： Verticillium dahliae causes a serious wilt disease of important crops and is difficult to control. Few plasma-membrane transport proteins for nutrient acquisition have been identified for this fungus, and their involvement in the disease process is unknown. Here, a plasma-membrane protein, the V dahliae thiamine transporter protein VdThit, was characterized functionally by deletion of the VdThit gene in V dahliae. Disruption strains were viable, but growth and conidial germination and production were reduced and virulence was impaired. Interestingly, by supplementing exogenous thiamine, growth, conidiation, and virulence of the Vd Delta Thit mutants were partially restored. Stress-tolerance assays showed that the Vd Delta Thit mutant strains were markedly more susceptible to oxidative stress and UV damage. High-pressure liquid chromatography-mass spectrometry (HPLC-MS) and gas chromatography-mass spectrometry (GC-MS) analyses showed low levels of pyruvate metabolism intermediates acetoin and acetyl coenzyme A (acetyl-CoA) in the Vd Delta Thit mutant strains, suggesting that pyruvate metabolism was suppressed. Expression analysis of VdThit confirmed the importance of VdThit in vegetative growth, reproduction, and invasive hyphal growth. Furthermore, a green fluorescent protein (GFP)-labeled Vd Delta Thit mutant (Vd Delta Thit-7-GFP) was suppressed in initial infection and root colonization, as viewed with light microscopy. Together, these results showed that VdThit plays an indispensable role in the pathogenicity of V dahliae.

分类号： Q94

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