In vitro protective efficacy of Lithium chloride against Mycoplasma hyopneumoniae infection

文献类型： 外文期刊

第一作者： Ishag, Hassan Z. A.

作者： Ishag, Hassan Z. A.;Wu, Yu-zi;Liu, Mao-jun;Xiong, Qi-yan;Feng, Zhi-xin;Yang, Ruo-song;Shao, Guo-qing;Ishag, Hassan Z. A.

作者机构：

关键词： Mycoplasma hyopneumoniae;LiCl;Inhibition;Apoptosis

期刊名称：RESEARCH IN VETERINARY SCIENCE （ 影响因子：2.534； 五年影响因子：2.382 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Mycoplasma hyopneumoniae (M. hyopneumoniae) infection affects the swine industry. Lithium chloride (LiCl), is a drug used to treat bipolar disorder and has also shown activity against bacterial and viral infections. Herein, we evaluated the antibacterial activity of LiCl on PK-15 cells infected with M. hyopneumoniae. Incubation of LiCI (40 mM) with cells for 24 h, did not significantly affect the cell viability. The qRT-PCR showed similar to 80% reduction in M. hyopneumoniae genome when LiCl added post-infection. A direct effect of LiCl on bacteria was also observed. However, treatment of cells with LiCl prior infection, does not protect against the infection. Antibacterial activity of LiCl was further confirmed by IFA, which demonstrated a reduction in the bacterial protein. With 40 mM LiCl, the apoptotic cell death, production of nitric oxide and superoxide anion induced by M. hyopneumoniae, were prevented by similar to 80%, 60% and 58% respectively. Moreover, caspase-3 activity was also reduced (82%) in cells treated with 40 mM LiCl. LiCl showed activity against various strains of M. hyopneumoniae examined in our study. Collectively, our data showed that LiCl inhibited the infection of M. hyopneumoniae through anti-apoptotic mechanism. (C) 2016 Elsevier Ltd. All rights reserved.

分类号： S85

  • 相关文献

[1]Apoptosis induced by lipid-associated membrane proteins from Mycoplasma hyopneumoniae in a porcine lung epithelial cell line with the involvement of caspase 3 and the MAPK pathway. Ni, B.,Bai, F. F.,Wei, Y.,Liu, M. J.,Feng, Z. X.,Xiong, Q. Y.,Hua, L. Z.,Shao, G. Q.. 2015

[2]Novel antiviral effect of lithium chloride on mammalian orthoreoviruses in vitro. Chen, Ye,Cai, Gengyuan,Jiang, Zhiguo,Jiao, Yiren,Shi, Yuzhen,Li, Huaqin,Wang, Chong,Kong, Deyang.

[3]Lithium chloride inhibits early stages of foot-and-mouth disease virus (FMDV) replication in vitro. Zhao, Fu-Rong,Xie, Yin-Li,Liu, Ze-Zhong,Shao, Jun-Jun,Li, Shi-Fang,Zhang, Yong-Guang,Chang, Hui-Yun,Zhao, Fu-Rong,Xie, Yin-Li,Liu, Ze-Zhong,Shao, Jun-Jun,Li, Shi-Fang,Zhang, Yong-Guang,Chang, Hui-Yun,Zhao, Fu-Rong. 2017

[4]Development and validation of an attenuated Mycoplasma hyopneumoniae aerosol vaccine. Feng, Zhi-Xin,Wei, Yan-Na,Li, Gui-Lan,Lu, Xiao-Ming,Wang, Zhan-Wei,Kong, Meng,Gan, Yuan,Bai, Fang-Fang,Liu, Mao-Jun,Xiong, Qi-Yan,Wu, Xu-Su,Shao, Guo-Qing,Wan, Xiu-Feng,Pharr, G. Todd. 2013

[5]A rapid and sensitive loop-mediated isothermal amplification procedure (LAMP) for Mycoplasma hyopneumoniae detection based on the p36 gene. Liu, M. J.,Du, G. M.,Bai, F. F.,Wu, Y. Z.,Xiong, Q. Y.,Feng, Z. X.,Li, B.,Shao, G. Q.,Du, G. M.,Liu, M. J.,Shao, G. Q.. 2015

[6]Immune Responses to the Attenuated Mycoplasma hyopneumoniae 168 Strain Vaccine by Intrapulmonic Immunization in Piglets. Feng Zhi-xin,Shao Guo-qing,Liu Mao-jun,Wu Xu-su,Zhou Yong-qi,Gan Yuan. 2010

[7]Cholesterol exacerbates Mycoplasma hyopneumoniae-induced apoptosis via stimulating proliferation and adhesion to porcine alveolar macrophages. Liu, Maojun,Hu, Yun,Liu, Jie,Jia, Yimin,Zhao, Ruqian,Liu, Maojun,Liu, Beibei,Shao, Guoqing,Jia, Yimin,Zhao, Ruqian,Du, Gaimei,Minion, F. Chris,Shao, Guoqing. 2017

[8]CYP1A1 mediates the suppression of major inflammatory cytokines in pulmonary alveolar macrophage (PAM) cell lines caused by Mycoplasma hyponeumoniae. Fang, Xiaomin,Zhao, Weimin,Xu, Jie,Tu, Feng,Wang, Xuemin,Li, Bixia,Fu, Yanfeng,Ren, Shouwen.

[9]Loop-mediated isothermal amplification for rapid and convenient detection of Mycoplasma hyopneumoniae. Li, Jiahe,Jiang, Fei,Yang, Sheng,Guo, Panpan,Wu, Wenxue,Minion, F. Chris,Petersen, Andrew C.,Li, Jinxiang.

[10]Mycoplasma hyopneumoniae induces pro-inflammatory cytokine and nitric oxide production through NF kappa B and MAPK pathways in RAW264.7 cells. Hwang, Mi-Hyun,Damte, Dereje,Lee, Joong-Su,Gebru, Elias,Chang, Zhi-Qiang,Rhee, Man-Hee,Park, Seung-Chun,Cheng, Henrique,Jung, Byeong-Yeal,Chang, Zhi-Qiang. 2011

[11]Development of a blocking ELISA for detection of Mycoplasma hyopneumoniae infection based on a monoclonal antibody against protein P65. Liu, Maojun,Zhang, Yue,Wu, Yuzi,Wang, Haiyan,Li, Bin,Bai, Yun,Feng, Zhixin,Xiong, Qiyan,Bai, Fangfang,Shao, Guoqing,Liu, Maojun,Du, Gaimei,Browning, Glenn F.,Shao, Guoqing.

[12]Effect of different adjuvant formulations on the immunogenicity and protective effect of a live Mycoplasma hyopneumoniae vaccine after intramuscular inoculation. Wang, Chunlai,Xie, Fang,Xiong, Qiyan,Wei, Yanna,Xie, Haidong,Feng, Zhixin,Gan, Yuan,Liu, Maojun,Bai, Fangfang,Shao, Guoqing.

[13]Comparative genomic analyses of Mycoplasma hyopneumoniae pathogenic 168 strain and its high-passaged attenuated strain. Liu, Wei,Xiao, Shaobo,Li, Mao,Guo, Shaohua,Li, Sha,Luo, Rui,Chen, Huanchun,Fang, Liurong,Feng, Zhixin,Li, Bin,Shao, Guoqing,Zhou, Zhemin. 2013

[14]Comparative analysis of mucosal immunity to Mycoplasma hyopneumoniae in Jiangquhai porcine lean strain and DLY piglets. Hua, L. Z.,Wu, Y. Z.,Bai, F. F.,William, K. K.,Feng, Z. X.,Liu, M. J.,Yao, J. T.,Zhang, X.,Shao, G. Q.. 2014

[15]Vaccination inhibits TLR2 transcription via suppression of GR nuclear translocation and binding to TLR2 promoter in porcine lung infected with Mycoplasma hyopneumoniae. Sun, Zhiyuan,Liu, Maojun,Zou, Huafeng,Li, Xian,Zhao, Ruqian,Liu, Maojun,Shao, Guoqing. 2013

[16]A replicating plasmid-based vector for GFP expression in Mycoplasma hyopneumoniae. Ishag, H. Z. A.,Liu, M. J.,Yang, R. S.,Xiong, Q. Y.,Feng, Z. X.,Shao, G. Q.,Ishag, H. Z. A.. 2016

[17]Protective efficacy of a live attenuated Mycoplasma hyopneumoniae vaccine with an ISCOM-matrix adjuvant in pigs. Xiong, Qiyan,Wei, Yanna,Feng, Zhixin,Gan, Yuan,Liu, Zhanjun,Liu, Maojun,Bai, Fangfang,Shao, Guoqing.

[18]The effects of Mycoplasma hyopneumoniae on porcine circovirus type 2 replication in vitro PK-15 cells. Wang, Haiyan,Feng, Zhixin,Wu, Yuzi,Wei, Yanna,Gan, Yuan,Hua, Lizhong,Li, Bin,Wang, Xiaomin,Liu, Maojun,Xiong, Qiyan,Shao, Guoqing,Wang, Haiyan,Feng, Zhixin,Shao, Guoqing.

[19]Use of serological and mucosal immune responses to Mycoplasma hyopneumoniae antigens P97R1, P46 and P36 in the diagnosis of infection. Feng, Zhi-Xin,Bai, Yun,Yao, Jing-Ting,Gan, Yuan,Wang, Hai-Yan,Wei, Yan-Na,Liu, Mao-Jun,Xiong, Qi-Yan,Bai, Fang-Fang,Li, Bin,Wu, Xu-Su,Shao, Guo-Qing,Feng, Zhi-Xin,Bai, Yun,Liu, Mao-Jun,Shao, Guo-Qing,Pharr, G. Todd,Wan, Xiu-Feng,Xiao, Shao-Bo,Chi, Ling-Zhi.

[20]Effects of Mycoplasma hyopneumoniae on porcine nasal cavity dendritic cells. Shen, Yumeng,Hu, Weiwei,Yang, Qian,Wei, Yanna,Feng, Zhixin.

作者其他论文 更多>>

微信小程序