Dietary supplementation of probiotic Bacillus PC465 isolated from the gut of Fenneropenaeus chinensis improves the health status and resistance of Litopenaeus vannamei against white spot syndrome virus

文献类型： 外文期刊

第一作者： Chai, Peng-Cheng

作者： Chai, Peng-Cheng;Song, Xiao-Ling;Xu, Hua;Huang, Jie;Huang, Jie;Chen, Guo-Fu

作者机构：

关键词： Probiotic;Litopenaeus vannamei;Growth;Survival rate;Digestive enzymes;Interior surface structure;Immune status;WSSV infection

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： This study conducted a 30-day feeding trial and a subsequent 20-day anti-virus infection trial to determine the effects of probiotic Bacillus PC465 on the growth, health status, and disease resistance of Litopenaeus vannamei. Shrimp samples were fed with three practical diets prepared from shrimp feed containing varying probiotic doses [0 (control), 10(7), and 10(9) CFU g(-1)]. Probiotic supplementation significantly increased the weight gain and survival of L. vannamei (p < 0.05). The effect of 10(9) CFU g(-1) on the growth rate was higher than that of 10(7) CFU g(-1). Compared with those in the control group, the activities of digestive enzymes, such as amylase, protease, and lipase, in the shrimp mid-gut significantly increased in the probiotic-fed groups on days 15 and 30, except lipase on day 30. The influence of 10(9) CFU g(-1) on enzyme activities was also greater than that of 10(7) CFU g(-1)]. Scanning electron microscopy revealed folds and large ravines across the interior surface of the mid-gut, and the number of these folds and ravines increased significantly after the probiotic was administered. The probiotic treatment significantly (p < 0.05) enhanced the transcription of penaeidin 3a (Pen-3a), peroxinectin, C-type lectin 3 (Lec-3), and thioredoxin (Trx) in the hemocytes of L. vannamei. Likewise, probiotic treatment increased the transcription of hemocyanin in the hepatopancreas of L. vannamei. The probiotic treatment also significantly increased the transcription of prophenoloxidase (proPO) but decreased the transcription of crustin in hemocytes. By contrast, the same treatment failed to increase the transcription of Ras-related protein (Rab-6) in hemocytes. The number of species and biomass of Bacillus in the mid-gut were higher in the probiotic-fed group than in the control group. The total biomass of microbes was higher in the shrimp fed with 10(7) CFU g(-1), than in the shrimp fed with 10(9) CFU g(-1), and the control group on days 15 and 30 post-feeding. In two white spot syndrome virus (WSSV) infections, the weight gain, survival, and WSSV copies within the gills of the probiotic-treated shrimp significantly differed (p < 0.05) from those of the control group. Relatively efficient protection was associated with probiotic feeding. Results suggested that Bacillus PC465 feeding improves the growth performance, survival, digestion, and nutrient absorption of L. vannamei. Probiotic treatment also enhances the microbial structures in the gut, promotes the immune status of shrimp, and provides protection against viral infection. The supplementation with 10(9) CFU g(-1) can also improve the growth and survival of L. vannamei. (C) 2016 Elsevier Ltd. All rights reserved.

分类号： S9

  • 相关文献

[1]Effects of dietary chitosan oligosaccharides and Bacillus coagulans on the growth, innate immunity and resistance of koi (Cyprinus carpio koi). Lin, Shimei,Mao, Shuhong,Guan, Yong,Luo, Li,Pan, Yu,Lin, Shimei,Luo, Li,Luo, Lin. 2012

[2]Food ingestion, consumption and selectivity of pompano, Trachinotus ovatus (Linnaeus 1758) under different rotifer densities. Ma, Zhenhua,Guo, Huayang,Zhang, Dianchang,Jiang, Shigui,Ma, Zhenhua,Hu, Chaoqun.

[3]Excess dietary cholesterol may have an adverse effect on growth performance of early post-larval Litopenaeus vannamei. Niu, Jin,Lin, Hei-Zhao,Chen, Peng-Fei,Tian, Li-Xia,Liu, Yong-Jian,Yang, Hui-Jun,Liang, Gui-Ying. 2012

[4]Effects of bioflocs on water quality, and survival, growth and digestive enzyme activities of Litopenaeus vannamei (Boone) in zero-water exchange culture tanks. Xu, Wu Jie,Pan, Lu Qing,Sun, Xiao Hua,Huang, Jie. 2013

[5]Effects of seawater potassium concentration on the dietary potassium requirement of Litopenaeus vannamei. Zhu, Chang-Bo,Dong, Shuang-Lin,Wang, Fang,Zhang, Han-Hua. 2006

[6]Effect of periodic light color change on the molting frequency and growth of Litopenaeus vannamei. Guo, Biao,Wang, Fang,Dong, Shuanglin,Guo, Biao,Mu, Yingchun. 2012

[7]Effects of dietary chitosan on growth, survival and stress tolerance of postlarval shrimp, Litopenaeus vannamei. Niu, J.,Liu, Y-J,Yang, H-J,Liang, G-Y,Tian, L-X,Niu, J.,Lin, H-Z,Mai, K-S. 2011

[8]Effects of dietary medicinal herbs and Bacillus on survival, growth, body composition, and digestive enzyme activity of the white shrimp Litopenaeus vannamei. Yu, Ming-Chao,Li, Zhuo-Jia,Lin, Hei-Zhao,Wen, Guo-Liang,Ma, Shen.

[9]Artificial substrates in zero-water-exchange culture system regulate the rearing performance of Pacific white shrimp Litopenaeus vannamei (Boone, 1931) under the winter indoor condition. Zhang, Jiasong,Chen, Liangliang,Dong, Hongbiao,Duan, Yafei,Li, Zhuojia,Wen, Guoliang,Chen, Jianguang,Feng, Zhenhua,Chen, Liangliang,Xu, WenJun,Xie, JianJun.

[10]The interaction of salinity and Na/K ratio in seawater on growth, nutrient retention and food conversion of juvenile Litopenaeus vannamei. Zhu, CB,Dong, SL,Wang, F. 2006

[11]Preliminary investigation into the contribution of bioflocs on protein nutrition of Litopenaeus vannamei fed with different dietary protein levels in zero-water exchange culture tanks. Xu, Wu-Jie,Pan, Lu-Qing,Zhao, Da-Hu,Huang, Jie.

[12]Effects of replacement of fish meal with fermented cottonseed meal on growth performance, body composition and haemolymph indexes of Pacific white shrimp, Litopenaeus vannamei Boone, 1931. Sun, Hong,Tang, Jiang-wu,Yao, Xiao-Hong,Wu, Yi-fei,Wang, Xin,Liu, Yong.

[13]Effect of long-term administration of dietary beta-1,3-glucan on growth, physiological, and immune responses in Litopenaeus vannamei (Boone, 1931). Zhao, Hong-Xia,Wang, An-Li,Ye, Chao-Xia,Zhao, Hong-Xia,Cao, Jun-Ming,Huang, Yan-Hua,Lan, Han-Bing,Zhou, Ting-Ting,Li, Guo-Li,Du, Zhen-Yu.

[14]Effect of salinity on survival, growth, oxygen consumption and ammonia-N excretion of juvenile whiteleg shrimp, Litopenaeus vannamei. Zhang, Peidong,Zhang, Xiumei,Gao, Tianxiang,Zhang, Peidong,Li, Jian.

[15]Effect of dietary crude protein level on villous morphology, immune status and histochemistry parameters of digestive tract in weaning piglets. Gu, X,Li, D. 2004

[16]Effects of curcumin on growth performance, jejunal mucosal membrane integrity, morphology and immune status in weaned piglets challenged with enterotoxigenic Escherichia coli. Xun, Wenjuan,Shi, Liguang,Zhou, Hanlin,Hou, Guanyu,Cao, Ting,Zhao, Chunping. 2015

[17]Effects of Arginine Supplementation on Organ Development, Egg Quality, Serum Biochemical parameters, and Immune Status of Laying Hens. Yang, H.,Ju, X.,Wang, Z.,Yang, Z.,Wang, W.,Lu, J.. 2016

[18]Effects of stocking density on growth performance, carcass yield, and immune status of a local chicken breed. Tong, H. B.,Lu, J.,Zou, J. M.,Wang, Q.,Shi, S. R.. 2012

[19]Effects of Dietary Arachidonic Acid Levels on Growth Performance, Whole-Body Proximate Composition, Digestive Enzyme Activities and Gut Morphology of Juvenile Golden Pompano Trachinotus ovatus.. Qi, Chang-Le,Lin, Hei-Zhao,Huang, Zhong,Zhou, Chuan-Peng,Wang, Yun,Wang, Jun,Niu, Jin,Tan, Xiao-Hong,Zhao, Shu-Yan,Qi, Chang-Le,Tan, Xiao-Hong,Zhao, Shu-Yan,Lin, Hei-Zhao,Huang, Zhong. 2016

[20]Tannins in livestock feeds in China. Diao, QY,Qi, GG. 2000

作者其他论文 更多>>

微信小程序