Determination of tetraiodo-L-thyronine in human serum by competitive indirect chemiluminescence immunoassay

文献类型： 外文期刊

第一作者： Gao, Yilin

作者： Gao, Yilin;Deng, Zhirui;Chen, Qin;Wang, Quan

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期刊名称：ANALYTICAL METHODS （ 影响因子：2.896； 五年影响因子：2.716 ）

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收录情况： SCI

摘要： Based on the monoclonal antibodies against 3,3',5,5'-tetraiodo-L-thyronine (T-4), a competitive indirect chemiluminescence enzyme immunoassay (CLEIA) used for the quantitative determination of total T-4 in human serum was established. The relevant results indicated that the determined coating antigen concentration was 2.7 mu g mL(-1) and the CLEIA titer of ascites was 1 : 8.0 x 10(4). The standard curve was y = -0.495x + 1.095, with a coefficient of determination, R-2 = 0.988, and the derived half inhibition concentration (IC50) of T-4 was 16.0 ng mL(-1). The system detection limit was 9.23 ng mL(-1). The mean inter- and intra-assay variations were 5.89% and 4.02%, respectively. The detected data of total T-4 in human serum from seven patients by this method exhibited significant correlation with reported clinical data, suggesting that this CLEIA method could be applicable to the clinical diagnosis of thyroid gland disease.

分类号： O65

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