Deletion of the BAC sequences from recombinant meq-null Marek's disease (MD) virus increases immunosuppression while maintaining protective efficacy against MD

文献类型: 外文期刊

第一作者: Su, S.

作者: Su, S.;Cui, N.;Sun, P.;Cui, Z.;Li, J.;Li, H.;Li, Y.

作者机构:

关键词: Marek's disease virus;meq-null;BAC;immunosuppression;protective efficacy

期刊名称:POULTRY SCIENCE ( 影响因子:3.352; 五年影响因子:3.679 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: Marek's disease virus (MDV) GX0101 is a field strain of MDV with a naturally occurring insertion of the reticuloendotheliosis virus (REV) long terminal repeat (LTR) fragment. Both copies of the meq gene were knocked out in the GX0101 bacterial artificial chromosome (BAC) clone to construct the recombinant virus SC9-1, resulting in a complete lack of pathogenicity and providing better protection against MD than CVI988/Rispens. In the present study, the BAC sequences in SC9-1 were removed using a cre-loxP system, and the virus termed SC9-2. SC9-2 showed a significant increase in replication in vitro and in vivo. There was a significant decrease in chicken weight, immune organ index, and antibody levels compared with those of SC9-1-inoculated chickens. The immune protection index of SC9-2 was similar to that of SC91, and the difference was not significant. The results of our studies demonstrate that the SC9-2 virus provides protection in specific pathogen free (SPF) chickens when challenged with a very virulent MDV rMd5, but it induces immunosuppressive effects in SPF chickens.

分类号: S8

  • 相关文献

[1]Evaluation of two strains of Mareles disease virus serotype 1 for the development of recombinant vaccines against very virulent infectious bursal disease virus. Li, Kai,Liu, Yongzhen,Liu, Changjun,Gao, Li,Gao, Yulong,Zhang, Yanping,Cui, Hongyu,Qi, Xiaole,Zhong, Li,Wang, Xiaomei.

[2]Cloning of equine herpesvirus type 1 438/77 strain genome as a bacterial artificial chromosome. Sun, Xuegiang,Yao, Huochun,Lu, Chengping,Zhang, Cun.

[3]A Gossypium BAC clone contains key repeat components distinguishing sub-genome of allotetraploidy cottons. Yuling Liu,Renhai Peng,Fang Liu,Xingxing Wang,Xinglei Cui,Zhongli Zhou,Chunying Wang,Xiaoyan Cai,Yuhong Wang,Zhongxu Lin,Kunbo Wang. 2016

[4]Screening and chromosome localization of two cotton BAC clones. Xinglei Cui,Fang Liu,Yuling Liu,Zhongli Zhou,Chunying Wang,Yanyan Zhao,Fei Meng,Xingxing Wang,Xiaoyan Cai,Yuhong Wang,Renhai Peng,Kunbo Wang. 2016

[5]Characterization of the cyp19a1a gene from a BAC sequence in half-smooth tongue sole (Cynoglossus semilaevis) and analysis of its conservation among teleosts. Shao Changwei,Shao Changwei,Liu Shanshan,Liu Changlin,Chen Songlin,Liu Geng. 2013

[6]Cytogenetic maps of homoeologous chromosomes A(h)01 and D(h)01 and their integration with the genome assembly in Gossypium hirsutum. Yuling Liu,Liu, Fang,Wang, Kunbo,Zhen Liu,Renhai Peng,Yuhong Wang,Zhongli Zhou,Xiaoyan Cai,Xingxing Wang,Zhenmei Zhang,Kunbo Wang,Fang Liu. 2017

[7]Construction of the BAC Library of Small Abalone (Haliotis diversicolor) for Gene Screening and Genome Characterization. Jiang, Likun,Xu, Jian,Jiang, Yanliang,Wang, Kai,Zhao, Zixia,Chen, Baohua,Zhao, Yunfeng,Xu, Peng,Jiang, Likun,Xu, Jian,Jiang, Yanliang,Wang, Kai,Zhao, Zixia,Chen, Baohua,Zhao, Yunfeng,Xu, Peng,You, Weiwei,Ke, Caihuan,Zhang, Xiaojun,Jiang, Likun,Chen, Baohua,Mahboob, Shahid,Al-Ghanim, Khalid A.,Mahboob, Shahid.

[8]Virus-encoded miR-155 ortholog is an important potential regulator but not essential for the development of lymphomas induced by very virulent Marek's disease virus. Yu, Zu-Hua,Teng, Man,Yu, Le-Le,Hu, Bo,Zhang, Gai-Ping,Luo, Jun,Yu, Zu-Hua,Hu, Bo,Liu, Ju-Xiong,Zhang, Gai-Ping,Sun, Ai-Jun,Cui, Zhi-Zhong,Qu, Liang-Hu,Yu, Zu-Hua,Ding, Ke,Cheng, Xiang-Chao,Luo, Jun.

[9]Construction and targeted retrieval of specific clone from a non-gridded soybean bacterial artificial chromosome library. Xia, Zhengjun,Wu, Hongyan,Xia, Zhengjun,Watanabe, Satoshi,Harada, Kyuya.

[10]The integrity of PRRSV nucleocapsid protein is necessary for up-regulation of optimal interleukin-10 through NF-kappa B and p38 MAPK pathways in porcine alveolar macrophages. Liu, Yanyan,Zhang, Yuyu,Zhu, Xiwang,Ren, Sufang,Guo, Lihui,Sun, Wenbo,Chen, Zhi,Cong, Xiaoyan,Chen, Lei,Du, Yijun,Li, Jun,Wu, Jiaqiang,Wang, Jinbao,Yu, Jiang,Liu, Yanyan,Zhang, Yuyu,Zhu, Xiwang,Ren, Sufang,Guo, Lihui,Sun, Wenbo,Chen, Zhi,Cong, Xiaoyan,Chen, Lei,Shi, Jianli,Du, Yijun,Li, Jun,Wu, Jiaqiang,Wang, Jinbao,Liu, Yanyan,Zhu, Xiwang,Wu, Jiaqiang,Wang, Jinbao,Liu, Xing. 2017

[11]Mucosal Immune Responses against Live Newcastle Disease Vaccine in Immunosuppressed Chickens. Yan, Zhengui,Zhao, Qingyou,Fan, Ruifeng,Guo, Wenlong,Ma, Rongde,Wang, Xinjian,Zhu, Ruiliang,Du, Yijun. 2011

[12]Cordyceps militaris polysaccharides can enhance the immunity and antioxidation activity in immunosuppressed mice. Wang, Mi,Qin, Tao,Hu, Yuan Liang,Wang, Mi,Meng, Xin Yu,Le Yang, Rui,Wang, Xiao Yang,Zhang, Ke Yu,Fei, Chen Zhong,Li, Ying,Xue, Fei Qun.

[13]NE Strengthens the Immunosuppression Induced by AlCl3 Through beta(2)-AR/cAMP Pathway in Cultured Rat Peritoneal Macrophages. Li, Miao,Yang, Xu,Zhuang, Cuicui,Cao, Zheng,Ren, Limin,Xiu, Chunyu,Li, Yanfei,Zhu, Yanzhu.

[14]Protective Effect of Chitosan Oligosaccharides Against Cyclophosphamide-Induced Immunosuppression and Irradiation Injury in Mice. Zhai, Xingchen,Yang, Xin,Zou, Pan,Wang, Jing,Zhai, Xingchen,Zou, Pan,Shao, Yong,Wang, Jing,Zhai, Xingchen,Yuan, Shoujun,Abd El-Aty, A. M.. 2018

[15]Interaction of infectious bursal disease virus with the immune system of poultry. Rehman, Z. U.,Meng, C.,Ding, C.,Rehman, Z. U.,Umar, S.,Umar, S.,Munir, M..

[16]ALV-J infection induces chicken monocyte death accompanied with the production of IL-1 beta and IL-18. Dai, Manman,Liao, Ming,Feng, Min,Xie, Tingting,Li, Yuanfang,Ruan, Zhuohao,Zhang, Xiquan,Feng, Min,Xie, Tingting,Li, Yuanfang,Ruan, Zhuohao,Zhang, Xiquan,Shi, Meiqing. 2017

[17]Effect of epimedium polysaccharide-propolis flavone immunopotentiator on immunosuppression induced by cyclophosphamide in chickens. Fan, Yunpeng,Wang, Deyun,Liu, Jiaguo,Zhao, Xiaojuan,Luong Nguyen,Hu, Yuanliang,Fan, Yunpeng,Song, Xiaoping,Zhang, Weimin,Lu, Yu.

[18]Combined recombinant DNA vaccine results in significant protection against Mycobacterium tuberculosis. Pan, Y,Cai, H,Li, SX,Tian, X,Li, T,Zhu, YX. 2003

[19]Expression of truncated Babesia microti apical membrane protein 1 and rhoptry neck protein 2 and evaluation of their protective efficacy. Wang, Guanbo,Efstratiou, Artemis,Moumouni, Paul Franck Adjou,Liu, Mingming,Jirapattharasate, Charoonluk,Guo, Huanping,Gao, Yang,Suzuki, Hiroshi,Igarashi, Ikuo,Xuan, Xuenan,Cao, Shinuo,Zhou, Mo.

[20]Effects of different promoters on the protective efficacy of recombinant Marek's disease virus type 1 expressing the VP2 gene of infectious bursal disease virus. Li, Kai,Liu, Yongzhen,Liu, Changjun,Gao, Li,Zhang, Yanping,Gao, Yulong,Cui, Hongyu,Qi, Xiaole,Zhong, Li,Wang, Xiaomei.

作者其他论文 更多>>