OsHSD1, a hydroxysteroid dehydrogenase, is involved in cuticle formation and lipid homeostasis in rice

文献类型： 外文期刊

第一作者： Zhang, Zhe

作者： Zhang, Zhe;Cheng, Zhi-jun;Gan, Lu;Wu, Fu-qing;Lin, Qi-bing;Wang, Jiu-lin;Wang, Jie;Guo, Xiu-ping;Zhang, Xin;Zhao, Zhi-chao;Lei, Cai-lin;Zhu, Shan-shan;Wan, Jian-min;Zhang, Huan;Wang, Chun-ming;Wan, Jian-min

作者机构：

关键词： Cuticular wax;Hydroxysteroid dehydrogenase;Lipid;Oil body;Oryza sativa

期刊名称：PLANT SCIENCE （ 影响因子：4.729； 五年影响因子：5.132 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： Cuticular wax, a hydrophobic layer on the surface of all aerial plant organs, has essential roles in plant growth and survival under various environments. Here we report a wax-deficient rice mutant oshsd1 with reduced epicuticular wax crystals and thicker cuticle membrane. Quantification of the wax components and fatty acids showed elevated levels of very-long-chain fatty acids (VLCFAs) and accumulation of soluble fatty acids in the leaves of the oshsd1 mutant. We determined the causative gene OsHSD1, a member of the short-chain dehydrogenase reductase family, through map-based cloning. It was ubiquitously expressed and responded to cold stress and exogenous treatments with NaCl or brassinosteroid analogs. Transient expression of OsHSD1-tagged green fluorescent protein revealed that OsHSD1 localized to both oil bodies and endoplasmic reticulum (ER). Dehydrogenase activity assays demonstrated that OsHSD1 was an NAD(+)/NADP(+)-dependent sterol dehydrogenase. Furthermore, OsHSD1 mutation resulted in faster protein degradation, but had no effect on the dehydrogenase activity. Together, our data indicated that OsHSD1 plays a specialized role in cuticle formation and lipid homeostasis, probably by mediating sterol signaling. This work provides new insights into oil-body associated proteins involved in wax and lipid metabolism. (C) 2016 Elsevier Ireland Ltd. All rights reserved.

分类号： Q94

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