Quantitative estimation of the replication kinetics of genotype 2 PRRSV strains with different levels of virulence in vitro

文献类型： 外文期刊

第一作者： Dong, Jianguo

作者： Dong, Jianguo;Wang, Gang;Liu, Yonggang;Shi, Wenda;Wu, Jianan;Wen, Huiqiang;Wang, Shujie;Tian, Zhijun;Cai, Xuehui;Dong, Jianguo

作者机构：

关键词： PRRSV;RT-qPCR;Replication kinetics;Viral loads

期刊名称：JOURNAL OF VIROLOGICAL METHODS （ 影响因子：2.014； 五年影响因子：2.001 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Porcine reproductive and respiratory syndrome virus (PRRSV) has become an important pathogen for the swine industry, and has resulted in substantial economic losses. In 2006, highly pathogenic PRRSV (HP-PRRSV) belonging to genotype 2 was first identified in China. Here, the replication kinetics of genotype 2 PRRSV strains were estimated in vitro in MARC-145 cells and porcine alveolar macrophages (PAMs) using a TaqMan-based real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR) assay. The lower limit of detection was 10 copies/mu L, and the assay was linear between 10(1) and 10(8) copies/mu L. The intra-assay coefficients of variation were 0.81-1.36%, and the inter-assay coefficients of variation were 1.77-2.56%. Compared to the low pathogenicity CH-1a-F45 strain, the viral loads of the highly pathogenic HuN4-F45 strain were 10(0.5)-10(1.05) and 10(0.84)-10(1.35) times greater in MARC-145 cells and PAMs, respectively from 12 to 9611 after infection (P < 0.01). This study is the first to demonstrate that the HuN4-F45 strain replicated at higher levels than CH-1a-F45 in MARC-145 cells and PAMs, suggesting that HuN4-F45 has more robust virus amplification efficiency than CH-1a-F45 in vitro. (C) 2016 Elsevier B.V. All rights reserved.

分类号： R37

  • 相关文献

[1]A comparative study of pigeons and chickens experimentally infected with PPMV-1 to determine antigenic relationships between PPMV-1 and NDV strains. Guo, Hongbo,Liu, Xiaoli,Xu, Yang,Han, Zongxi,Shao, Yuhao,Kong, Xiangang,Liu, Shengwang.

[2]Comprehensive evaluation of candidate reference genes for gene expression studies in Lysiphlebia japonica (Hymenoptera: Aphidiidae) using RT-qPCR. Xue-Ke Gao,Cui, Jin-Jie,Shuai Zhang,Jun-Yu Luo,Chun-Yi Wang,Li-Min Lü,Li-Juan Zhang,Xiang-Zhen Zhu,Li Wang,Hui Lu,Jin-Jie Cui.

[3]Assessment of reference gene stability in Rice stripe virus and Rice black streaked dwarf virus infection rice by quantitative Real-time PCR. Fang, Peng,Sun, Feng,Lan, Ying,Du, Linlin,Zhou, Yijun,Zhou, Tong,Fang, Peng,Sun, Feng,Lan, Ying,Du, Linlin,Zhou, Yijun,Zhou, Tong,Fang, Peng,Lu, Rongfei,Shen, Wenbiao. 2015

[4]MicroRNAs associated with drought response in the pulse crop common bean (Phaseolus vulgaris L.). Wu, Jing,Wang, Lanfen,Wang, Shumin. 2017

[5]Molecular cloning and expression pattern of oriental river prawn (Macrobrachium nipponense) nitric oxide synthase. Rahman, N. M. A.,Fu, H. T.,Jin, S.,Bai, H. K.,Liang, G. X.,Gong, Y. S.,Wu, Y.,Fu, H. T.,Sun, S. M.,Qiao, H.,Jin, S.,Zhang, W. Y.,Xiong, Y. W.,Rahman, N. M. A.. 2016

[6]Molecular cloning and expression analysis of Fem1b from oriental river prawn Macrobrachium nipponense. Rahman, N. M. A.,Fu, H.,Bai, H.,Jiang, F. W.,Liang, G.,Jiang, F. F.,Wu, Y.,Fu, H.,Qiao, H.,Jin, S.,Zhang, W.,Sun, S.,Gong, Y.,Xiong, Y.,Rahman, N. M. A.. 2016

[7]A duplex, SYBR Green I-based RT-qPCR assay for the simultaneous detection of Apple chlorotic leaf spot virus and Cherry green ring mottle virus in peach. Liang, Pengbo,Wang, Hongqing,Zhao, Zhe,Yu, Yun,Zhang, Zhixiang,Liang, Pengbo,Ma, Yuxin,Li, Shifang. 2013

[8]A one step real-time RT-PCR assay for the quantitation of Wheat yellow mosaic virus (WYMV). Liu, Wenwen,Zhao, Xiaojuan,Zhang, Peng,Thi Thi Mar,Liu, Yan,Wang, Xifeng,Zhang, Zongying,Han, Chenggui,Zhang, Zongying,Han, Chenggui. 2013

[9]Evaluation of putative internal reference genes for gene expression normalization in Nannochloropsis sp by quantitative real-time RT-PCR. Cao, Shaona,Zhang, Xiaowen,Ye, Naihao,Fan, Xiao,Xu, Dong,Cao, Shaona,Wang, Yitao,Wang, Wenqi,Liang, Chengwei. 2012

[10]RT-qPCR analysis of dexB and galE gene expression of Streptococcus alactolyticus in Astragalus membranaceus fermentation. Hao, Gui J.,Zhang, Kai,Zhang, Jing Y.,Wang, Xu R.,Qin, Zhe,Wang, Xue Z.,Wang, Lei,Meng, Jia R.,Yang, Zhi Q.,Li, Jian X.,Hao, Gui J.,Zhang, Kai,Zhang, Jing Y.,Wang, Xu R.,Qin, Zhe,Wang, Xue Z.,Wang, Lei,Meng, Jia R.,Yang, Zhi Q.,Li, Jian X..

[11]Identification and comparative analysis of the Pseudosciaena crocea microRNA transcriptome response to poly(I:C) infection using a deep sequencing approach. Qi, Pengzhi,Guo, Baoying,Zhu, Aiyi,Wu, Changwen,Liu, Changlin.

[12]Characterization, expression and silencing by RNAi of p53 from Penaeus monodon. Dai, Wenting,Qiu, Lihua,Zhao, Chao,Fu, Mingjun,Ma, Zhenhua,Zhou, Falin,Yang, Qibin,Qiu, Lihua,Zhao, Chao,Fu, Mingjun,Ma, Zhenhua,Zhou, Falin,Yang, Qibin,Dai, Wenting,Yang, Qibin.

[13]Cloning, expression and methylation analysis of piwil2 in half-smooth tongue sole (Cynoglossus semilaevis). Zhang, Liyan,Zhao, Wen,Zhang, Liyan,Liu, Wanjun,Shao, Changwei,Zhang, Ning,Li, Hailong,Liu, Kun,Dong, Zhongdian,Qi, Qian,Chen, Songlin,Liu, Wanjun,Dong, Zhongdian,Qi, Qian.

[14]Quantification of southern rice black streaked dwarf virus and rice black streaked dwarf virus in the organs of their vector and nonvector insect over time. Wang, Biao,Wang, Xifeng,Ren, Yingdang,Lu, Chuantao.

[15]A qPCR aptasensor for sensitive detection of aflatoxin M-1. Guo, Xiaodong,Wen, Fang,Zheng, Nan,Li, Songli,Wang, Jiaqi,Guo, Xiaodong,Fauconnier, Marie-Laure,Guo, Xiaodong,Wen, Fang,Zheng, Nan,Li, Songli,Wang, Jiaqi,Zheng, Nan,Wang, Jiaqi.

[16]Validation of Reference Genes for RT-qPCR Studies of Gene Expression in Preharvest and Postharvest Longan Fruits under Different Experimental Conditions. Wu, Jianyang,Zhang, Hongna,Liu, Liqin,Li, Weicai,Wei, Yongzan,Shi, Shengyou. 2016

[17]Evaluation of reference genes for quantitative real-time RT-PCR analysis of gene expression in Nile tilapia (Oreochromis niloticus). Yang, Chang Geng,Tian, Juan,Liu, Wei,Wu, Fan,Jiang, Ming,Wen, Hua,Wang, Xian Li.

[18]Direct and efficient cloning of full-length genes from environmental DNA by RT-qPCR and modified TAIL-PCR. Huang, Huoqing,Wang, Guozeng,Zhao, Yanyu,Shi, Pengjun,Luo, Huiying,Yao, Bin.

[19]Analysis of multiple transcriptomes of the African oil palm (Elaeis guineensis) to identify reference genes for RT-qPCR. Xia, Wei,Xiao, Yong,Liu, Zheng,Yang, Yaodong,Lei, Xintao,Ma, Zilong,Peng, Ming,Mason, Annaliese S.,Mason, Annaliese S.,Wu, Xiaoming.

[20]The validity of a reference gene is highly dependent on the experimental conditions in green alga Ulva linza. Zhang, Xiaowen,Xu, Dong,Ye, Naihao,Dong, Meitao,Wang, Wenqi,Chi, Xiaoyuan,Mou, Shanli,Xu, Jianfang.

作者其他论文 更多>>

微信小程序