Reverse transcription-PCR assays for the differentiation of various US porcine epidemic diarrhea virus strains

文献类型： 外文期刊

第一作者： Liu, Xinsheng

作者： Liu, Xinsheng;Wang, Qiuhong;Liu, Xinsheng

作者机构：

关键词： Porcine epidemic diarrhea virus;Reverse transcription-PCR;Spike gene

期刊名称：JOURNAL OF VIROLOGICAL METHODS （ 影响因子：2.014； 五年影响因子：2.001 ）

ISSN：

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收录情况： SCI

摘要： Concurrently, several porcine epidemic diarrhea virus (PEDV) variants are circulating in US swine farms, including the original US and the spike insertion-deletion (S-INDEL) strains. In this study, reverse transcription (RT)-PCR assays for the detection and differentiation of different US PEDV variants were developed based on the differences in the S1 domain of the spike (S) gene. This assay successfully differentiated three PEDV strains: PC22A (the original US virulent), Iowa106 (S-INDEL), and PC177 (S-197DEL) that was derived from cell culture adaptation and has a 197 amino acid-deletion in the S1 domain. The assays did not amplify the porcine deltacoronavirus OH-FD22 strain or transmissible gastroenteritis virus Miller strain. It is the first report on the development of RT-PCR assays allowing the detection and differentiation of all major types of US PEDV variants. (C) 2016 Elsevier B.V. All rights reserved.

分类号： R37

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