Cloning and expression profile of ionotropic receptors in the parasitoid wasp Microplitis mediator (Hymenoptera: Braconidae)

文献类型: 外文期刊

第一作者: Dhiloo, Khalid Hussain

作者: Dhiloo, Khalid Hussain;Zheng, Yao;Shan, Shuang;Zhang, Yong-Jun;Guo, Yu-Yuan;Peng, Yong;Li, Rui-Jun;Lu, Zi-Yun;Dhiloo, Khalid Hussain;Shan, Shuang

作者机构:

关键词: Microplitis mediator;Antennae;Ionotropic receptor;Expression profile;Olfactory sensory neuron

期刊名称:JOURNAL OF INSECT PHYSIOLOGY ( 影响因子:2.354; 五年影响因子:3.045 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: Ionotropic receptors (IRs) mainly detect the acids and amines having great importance in many insect species, representing an ancient olfactory receptor family in insects. In the present work, we performed RNAseq of Microplitis mediator antennae and identified seventeen IRs. Full-length MmedIRs were cloned and sequenced. Phylogenetic analysis of the Hymenoptera IRs revealed that ten MmedIR genes encoded "antennal IRs" and seven encoded "divergent IRs". Among the IR25a orthologous groups, two genes, MmedIR25a.1 and MmedIR25a.2, were found in M. mediator. Gene structure analysis of MmedIR25a revealed a tandem duplication of IR25a in M. mediator. The tissue distribution and development specific expression of the MmedIR genes suggested that these genes showed a broad expression profile. Quantitative gene expression analysis showed that most of the genes are highly enriched in adult antennae, indicating the candidate chemosensory function of this family in parasitic wasps. Using immunocytochemistry, we confirmed that one co-receptor, MmedlR8a, was expressed in the olfactory sensory neurons. Our data will supply fundamental information for functional analysis of the IRs in parasitoid wasp chemoreception. (C) 2016 Elsevier Ltd. All rights reserved.

分类号: Q965

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