Molecular mechanism of endocrine system impairment by 17 alpha-methyltestosterone in gynogenic Pengze crucian carp offspring

文献类型： 外文期刊

第一作者： Zheng, Yao

作者： Zheng, Yao;Liu, Yan;Gao, Jiancao;Yang, Yanping;Zhang, Yingying;Wang, Zaizhao;Zheng, Yao;Chen, Jiazhang;Yang, Yanping;Bing, Xuwen;Zheng, Yao;Chen, Jiazhang;Yang, Yanping;Bing, Xuwen;Zheng, Yao;Chen, Jiazhang;Yang, Yanping;Bing, Xuwen;Zheng, Yao;Gao, Zexia;Liang, Hongwei

作者机构：

关键词： 17 alpha-methyltestosterone;Estrogen signalling;Gynogenic Pengze crucian carp;Steroid hormones;Steroidogenesis;Vitellogenesis

期刊名称：ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY （ 影响因子：6.291； 五年影响因子：6.393 ）

ISSN：

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收录情况： SCI

摘要： The effects of synthetic androgen 17 alpha-methyltestosterone (MT) on endocrine impairment were examined in crucian carp. Immature 7-month old mono-female Pengze crucian carp (Pcc) F2 offspring were exposed to 50 and 100 mu g/L of MT (week 2, 4, and 8). Gonadosomatic index, hepatosomatic index and intestine weight altered considerably and oocyte development was repressed. In the treatment groups, ovarian 11-ketotestosterone decreased, whereas 17 beta-estradiol and testosterone increased, and ovarian aromatase activities increased at week 4. However, in the brain tissue, those values, significantly decreased. Quantitative RT-PCR analysis demonstrated changes in steroid receptor genes and upregulation of steroidogenic genes (Pcc-3bhsd, Pcc-11bhsd2 Pcc-cyp11 alpha 1), while the other three steroidogenic genes (Pcc-cyp17 alpha 1, Pcc-cyp19a1a and Pcc-star) decreased from week 4 to week 8. Ovarian, hepatic Pcc-vtg B and vitellogenin concentration increased in both 50 and 100 a of MT exposure groups. This study adds further information regarding the effects of androgens on the development of previtellogenic oocytes, which suggests that MT could directly target estrogen signaling pathway, or indirectly affect steroidogenesis and vitellogenesis. (C) 2016 Elsevier Inc. All rights reserved.

分类号： X`R12`R99

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