Determination of astaxanthin in feeds using high performance liquid chromatography and an efficient extraction method

文献类型： 外文期刊

第一作者： Du, Pengfei

作者： Du, Pengfei;Jin, Maojun;Yang, Lihua;Chen, Ge;Zhang, Chan;Jin, Fen;Shao, Hua;Yang, Mao;She, Yongxin;Wang, Shanshan;Zheng, Lufei;Wang, Jing;Yang, Xin

作者机构：

关键词： Astaxanthin;enzymatic hydrolysis;feeds;HPLC;saponification

期刊名称：JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES （ 影响因子：1.312； 五年影响因子：1.145 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： A high performance liquid chromatography method using an efficient extraction method was developed for the determination of astaxanthin in eight kinds of animal feed. The chromatographic separation was achieved using a C-18 reversed-phase column, using methanol and acetonitrile as the mobile phases with a flow rate of 1 mL/min. The feeds containing astaxanthin were first treated with maxatase, to cause enzymatic hydrolysis, and then extracted with dichloromethane. The optimized method produced recoveries of between 82.4% and 100% for all eight kinds of feed, and the coefficients of variation were lower than 4.28%. The limit of detection, defined as the concentration that gave a signal-to-noise ratio of 3, for astaxanthin was estimated to be 0.1 mu g/g. The limit of quantification, defined as the lowest spiked concentration that gave an appropriate level of precision and accuracy, was 0.3 mu g/g. Finally, the method developed was used to determine astaxanthin in real, commercially sourced, feed samples. The method met the requirements for the determination of astaxanthin in feed, providing satisfactory recoveries of 70-110%.

分类号： O661

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