Generation of transgenic watermelon resistance to Cucumber mosaic virus facilitated by an effective Agrobacterium-mediated transformation method

文献类型： 外文期刊

第一作者： Liu, Lifeng

作者： Liu, Lifeng;Ijaz, Raina;Zhang, Junhong;Ye, Zhibiao;Liu, Lifeng;Gu, Qinsheng

作者机构：

关键词： Watermelon;Transformation;Cucumber mosaic virus;Resistance;Artificial microRNA

期刊名称：SCIENTIA HORTICULTURAE （ 影响因子：3.463； 五年影响因子：3.672 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Watermelon is an annual vegetable crop that suffers from various diseases during growth. Genetic engineering is an effective tool for improving plant disease resistance. This paper presents an effective transformation protocol designed for a female parent watermelon line mediated by Agrobacterium. In this protocol, 2 times MS Fe-EDTA were supplemented into Murashige and Skoog (MS) medium to suppress the chlorosis during watermelon explants culturing, and the optimal preculture time (5 days) and coculture time (5 days) were screened out to improve the frequency of shoots regeneration. Using our protocol, 17 putative transgenic plants expressing artificial microRNAs that target Cucumber mosaic virus (CMV) 2a/2b genes were recovered, and seven of them were succeeded in obtaining T1 generation seeds because of the technique difficulties of self-pollination of the transgenic watermelon lines. PCR and Southern blot analyses confirmed that the transferred fragment was successfully imported and integrated into the genome of three of these seven transgenic lines. DAS-ELISA assay indicated that the CMV can be detected in the nontransgenic line but not in the transgenic lines, and the transgenic lines displayed resistance to CMV infection. (C) 2016 Elsevier B.V. All rights reserved.

分类号： S6

  • 相关文献

[1]Engineered Bacillus thuringiensis GO33A with broad insecticidal activity against lepidopteran and coleopteran pests. Wang, Guangjun,Zhang, Jie,Song, Fuping,Wu, Jun,Feng, Shuliang,Huang, Dafang.

[2]The artificial microRNA mediates GUS-GFP gene silencing using ath-miR169d precursor as backbone. Liu, Chong,Zhang, Lan,Sun, Jie,Luo, Yanzhong,Fan, Yunliu,Wang, Lei,Liu, Chong,Sun, Jie,Wang, Mingbo. 2009

[3]A novel approach for the construction of plant amiRNA expression vectors. Yan, Hong,Deng, Xuan,Ma, Lixin,Yan, Hong,Zhao, Bin,Cao, Yinglong,Huang, Junyan.

[4]A simplified method for constructing artificial microRNAs based on the osa-MIR528 precursor. Yan, Fei,Lu, Yuwen,Wu, Gentu,Peng, Jiejun,Zheng, Hongying,Lin, Lin,Chen, Jianping,Wu, Gentu,Peng, Jiejun.

[5]Reduced weed seed shattering by silencing a cultivated rice gene: strategic mitigation for escaped transgenes. Yan, Huanxin,Li, Lei,Liu, Ping,Jiang, Xiaoqi,Wang, Lei,Fang, Jia,Lu, Bao-Rong,Lin, Zhimin,Wang, Feng,Su, Jun.

[6]Histological and Ultrastructural Observation Reveals Significant Cellular Differences between Agrobacterium Transformed Embryogenic and Non-embryogenic Calli of Cotton. Shang, Hai-Hong,Liu, Chuan-Liang,Zhang, Chao-Jun,Li, Feng-Lian,Hong, Wei-Dong,Li, Fu-Guang.

[7]Improved Resistance to Cucumber mosaic virus in Petunia Transformed with Non-Cytotoxic Pokeweed Antiviral Protein Gene. Li, Yu,Chen, Dinghu,Wang, Xifeng,Feng, Hui,Chen, Dinghu. 2013

[8]An efficient virus-induced gene silencing vector for maize functional genomics research. Wang, Rong,Yang, Xinxin,Wang, Nian,Liu, Xuedong,Fan, Zaifeng,Zhou, Tao,Wang, Rong,Yang, Xinxin,Wang, Nian,Liu, Xuedong,Fan, Zaifeng,Zhou, Tao,Nelson, Richard S.,Li, Weimin.

[9]Identification and characterization of a new cucumber mosaic virus isolate from passionfruit in Hainan of China. Liu, ZX,Pan, JS,Wei, YW,Wu, H,Zheng, XQ. 2004

[10]Problems Encountered with the Selection of Cucumber Mosaic Virus (CMV) Isolates for Resistance Breeding Programs. Adam, Guenter,Heinze, Cornelia,Zhang, Deyong,Tan, Xinqiu. 2011

[11]Multiple virus resistance using artificial trans-acting siRNAs. Chen, Lingyun,Cai, Jianyu,Zhan, Linlin,Wu, Xiaoyun,Cheng, Xiaofei,Wu, Xiaoxia,Liu, Qi.

[12]Co-digestion of tobacco waste with different agricultural biomass feedstocks and the inhibition of tobacco viruses by anaerobic digestion. Liu, Yi,Liu, Gangjin,Yang, Hongnan,Wang, Lan,Kong, Chuixue,Zheng, Dan,Deng, Liangwei,Dong, Jianxin,Liu, Wei,Yang, Jinguang,Wang, Shusheng.

[13]Aphid performance changes with plant defense mediated by Cucumber mosaic virus titer. Gao, Yang,Yan, Shuo,Tang, Xin,Zhang, Deyong,Liu, Yong,Zhang, Deyong,Liu, Yong,Zhou, Xuguo. 2016

[14]Detection of pepper mild mottle virus in pepper sauce in China. Peng, Jiejun,Zheng, Hongying,Lu, Yuwen,Lin, Lin,Chen, Jianping,Yan, Fei,Shi, Bingbin,Jiang, Tong.

[15]Transmission Comparisons of Cucumber Mosaic Virus Subgroup I and II Isolates by Different Aphid Species. Tian, Zhaofeng,Liu, Weicheng,Luo, Chen,Liu, Ting,Li, Yongdan.

[16]Inheritance of male-sterility and dwarfism in watermelon [Citrullus lanatus (Thunb.) Matsum. and Nakai]. Huang, HX,Zhang, XQ,Wei, ZC,Li, QH,Li, X. 1998

[17]Relationships between endogenous hormonal content and direct somatic embryogenesis in Watermelon (Citrullus lanatus) cotyledons. Zhang, Hui Jun,Wang, Qing,Wang, Qing. 2015

[18]Genetic Analysis on Fruit Cut Cracking of Watermelon. Jiang, Hai-kun,Zhang, Qi-an,Fang, Lin. 2010

[19]One-step reverse transcription loop-mediated isothermal amplification for the rapid detection of cucumber green mottle mosaic virus. Wei, Qi-wei,Zhang, Wen-na,Wu, Jian-yan,Charimbu, Miriam Karwitha,Hu, Bai-shi,Tao, Xiao-rong,Liu, Yong,Tan, Xin-qiu,Hu, Bai-shi,Cheng, Zhao-bang,Yu, Cui. 2013

[20]Histological differences between watermelon grafted onto bottle gourd rootstock and self-rooted seedlings inoculated with Fusarium oxysporum f. sp niveum. Zhang, M.,Yang, X. P.,Liu, G.,Xu, J. H.,Zhu, L. L.,Gao, C. Z.,Li, P. F.,Yao, X. F.. 2012

作者其他论文 更多>>

微信小程序