CHIP mediates down-regulation of nucleobindin-1 in preosteoblast cell line models

文献类型： 外文期刊

第一作者： Xue, Fuying

作者： Xue, Fuying;Zhao, Xinghui;Zhao, Taoran;Meng, Ying;Guo, Junwei;Chen, Wei;Wu, Yanping;Zhao, Zhanzhong

作者机构：

关键词： Nucleobindin-1;CHIP;Comparative two-dimensional gel;electrophoresis;Osteoblast differentiation;Heat shock proteins

期刊名称：CELLULAR SIGNALLING （ 影响因子：4.315； 五年影响因子：4.206 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Nucleobindin-1 (NUCB1), also known as Calnuc, is a highly conserved, multifunctional protein widely expressed in tissues and cells. It contains two EF-hand motifs which have been shown to play a crucial role in binding Ca2+ ions. In this study, we applied comparative two-dimensional gel electrophoresis to characterize differentially expressed proteins in HA-CHIP over-expressed and endogenous CHIP depleted MC3T3-E1 stable cell lines, identifying NUCB1 as a novel CHIP/Stub1 targeted protein. NUCB1 interacts with and is down-regulated by CHIP by both proteasomal dependent and independent pathways, suggesting that CHIP-mediated down regulation of nucleobindin-1 might play a role in osteoblast differentiation. The chaperone protein Hsp70 was found to be important for CHIP and NUCB1 interaction as well as CHIP-mediated NUCB1 down-regulation. Our findings provide new insights into understanding the stability regulation of NUCB1. (C) 2016 Elsevier Inc. All rights reserved.

分类号： Q24

  • 相关文献

[1]Cloning and expression of heat shock protein genes in two whitefly species in response to thermal stress. Wan, F. -H.,Yu, H.,Wan, F. -H..

[2]Functional characterization of GhSOC1 and GhMADS42 homologs from upland cotton (Gossypium hirsutum L.). Xiaohong Zhang,Jianghui wei,Shuli Fan,Meizhen Song,Chaoyou Pang,Hengling Wei,Chengshe Wang,Shuxun Yu. 2016

[3]Inhibition of osteoblast differentiation by aluminum trichloride exposure is associated with inhibition of BMP-2/Smad pathway component expression. Yang, Xu,Xiu, Chunyu,Song, Miao,Han, Yanfei,Li, Yanfei,Huo, Hui,Zhu, Yanzhu.

[4]Cloning and characterization of a FLO/LFY ortholog in Gossypium hirsutum L.. Jie Li,Shu-Li Fan,Mei-Zhen Song,Chao-You Pang,Heng-Ling Wei,Wei Li,Jian-hui Ma,Jiang-Hui Wei,Jian-guo Jing,Shu-Xun Yu.

[5]Loop-mediated isothermal amplification for visual detection of Vibrio parahaemolyticus using gold nanoparticles. Kong, Cong,Wang, Yuan,Yang, Guang-xin,Han, Feng,Fodjo, Essy Kouadio,Shen, Xiao-sheng. 2018

[6]Protein characteristics of Chinese black-grained wheat. Li, WD,Beta, T,Sun, SC,Corke, H. 2006

[7]Differentiation of the high molecular weight glutenin subunit D-t x2.1 of Aegilops tauschii indicated by partial sequences of its encoding gene and SSR markers. Yang, WY,Lu, BR. 2005

[8]Association between PCR-SSCP of bone morphogenetic protein 15 gene and prolificacy in Jining Grey goats. Chu, M. X.,Jiao, C. L.,He, Y. Q.,Wang, J. Y.,Liu, Z. H.,Chen, G. H..

[9]Comparative proteomics of leaves found at different stem positions of maize seedlings. Chen, Yi-Bo,Zhao, Biligen-Gaowa,Wang, Bai-Chen,Wang, Dan,Wang, Xu-Chu,Ge, Xuan-Liang.

[10]Analysis of the microflora in Tibetan kefir grains using denaturing gradient gel electrophoresis. Jiang Hanhu,Dong Mingsheng,Zhou Jianzhong,Liu Xiaoli.

[11]Overexpression of AtHsp90.3 in Arabidopsis thaliana impairs plant tolerance to heavy metal stress. Song, H. M.,Wang, H. Z.,Xu, X. B.,Song, H. M.. 2012

[12]Roles of heat-shock protein 70 in protecting against intestinal mucosal damage. Wu, Xin,Zhang, Yuzhe,Yin, Yulong,Wu, Xin,Yin, Yulong,Ruan, Zheng,Wu, Xin,Yin, Yulong,Ruan, Zheng,Yu, Huimin,Wu, Zhenlong,Wu, Guoyao,Wu, Guoyao. 2013

[13]Heat-induced apoptosis and gene expression in bovine mammary epithelial cells. Hu, Han,Wang, Jiaqi,Gao, Haina,Li, Songli,Zhang, Yangdong,Zheng, Nan,Hu, Han,Wang, Jiaqi,Gao, Haina,Li, Songli,Zhang, Yangdong,Zheng, Nan,Hu, Han,Wang, Jiaqi,Gao, Haina,Li, Songli,Zhang, Yangdong,Zheng, Nan.

[14]Molecular cloning, characterization, and heterologous expression analysis of heat shock protein genes (hsp70 and hsp90) of the invasive alien weed, Ageratina adenophora (Asteraceae). Wan, Fang Hao,Guo, Jian Ying,Gong, Wei Na,Xie, Bing Yan.

[15]Yeast heat-shock protein gene HSP26 enhances freezing tolerance in Arabidopsis. Xue, Yong,Peng, Rihe,Xiong, Aisheng,Li, Xian,Yao, Quanhong,Zha, Dingshi.

[16]Proteome Analysis on Differentially Expressed Proteins of the Fat Body of Two Silkworm Breeds, Bombyx mori, Exposed to Heat Shock Exposure. Moghaddam, S. Hossein Hosseini,Du, Xin,Li, Jun,Zhong, Boxiong,Chen, YuYin,Moghaddam, S. Hossein Hosseini,Cao, Jinru. 2008

[17]Effect of high dietary carbohydrate on growth, serum physiological response, and hepatic heat shock cognate protein 70 expression of the top-mouth culter Erythroculter ilishaeformis Bleeker. Liu, Bo,Xie, Jun,Ge, Xian-ping,Wang, Guangyu,Liu, Bo,Xie, Jun,Ge, Xian-ping,Miao, Ling-hong.

[18]Transient changes of enzyme activities and expression of stress proteins in the small intestine of piglets after weaning. Tao, Xin,Xu, Ziwei,Men, Xiaoming.

[19]Three heat shock proteins from Spodoptera exigua: Gene cloning, characterization and comparative stress response during heat and cold shocks. Xu, Qi,Zou, Qi,Zheng, Huizhen,Tang, Bin,Wang, Shigui,Zhang, Fan,Tang, Bin.

[20]Effects of heat stress on antioxidant defense system, inflammatory injury, and heat shock proteins of Muscovy and Pekin ducks: evidence for differential thermal sensitivities. Zeng, Tao,Wang, Gen-lin,Lu, Li-zhi,Zeng, Tao,Li, Jin-jun,Wang, De-qian,Li, Guo-qin,Lu, Li-zhi.

作者其他论文 更多>>

微信小程序