A novel detoxifying agent: Using rice husk carriers to immobilize zearalenone-degrading enzyme from Aspergillus niger FS10

文献类型： 外文期刊

第一作者： He, Mengling

作者： He, Mengling;Li, Yun;Pi, Fuwei;Ji, Jian;He, Xingxing;Zhang, Yinzhi;Sun, Xiulan;Li, Yun

作者机构：

关键词： Detoxifying agent;Immobilization;Zearalenone;Rice husk;Artificial porcine gastrointestinal digestive juice

期刊名称：FOOD CONTROL （ 影响因子：5.548； 五年影响因子：5.498 ）

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年卷期：

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收录情况： SCI

摘要： The greatest hidden peril for commercially detoxifying agents is that although a toxin binding to the adsorbent is discharged outside of the animal without causing damage to them, because the toxin is too stable to destroy, they accumulate in the soil or barn, and become secondary hazards to the environment and animals. In this study, a novel detoxifying agent which uses rice husk (RH) to immobilize zearalenone-degrading enzyme (ZDE) was produced to reduce zearalenone (ZEN). In addition, the ZDE exhibited high enhancement of both its stability and catalytic activity for removing ZEN. The immobilized ZDE is more resistant to thermal denaturation than the native enzyme. At 90 degrees C, it retained 70% ZEN removal rate compared to the native enzyme. Additionally, it was also more stable during storage at 4 degrees C and 25 degrees C: after one month the immobilized ZDE retained 90% and 70% of initial activity, respectively. Furthermore, a comparison of the removal rate of immobilized ZDE with other detoxifying agents in artificial porcine gastrointestinal digestive juice revealed that the ZEN removal efficacy of immobilized ZDE was higher than other detoxifying agents. Specifically, the removal efficacy of the immobilized ZDE was 75.45% for ZEN in gastric digestive juice and 90.43% in intestinal digestive juice. (c) 2016 Elsevier Ltd. All rights reserved.

分类号： TS2

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