Isolation of broad-specificity domain antibody from phage library for development of pyrethroid immunoassay
文献类型: 外文期刊
第一作者: Zhao, Yanyan
作者: Zhao, Yanyan;Zhao, Shengming;Tu, Kang;Liang, Ying;Liu, Yuan;Zhang, Xiao;Hu, Xiaodan;Wu, Aihua;Zhang, Cunzheng;Zhong, Jianfeng;Liu, Xianjin;Liang, Ying;Liu, Yuan;Zhang, Xiao;Hu, Xiaodan;Wu, Aihua;Zhang, Cunzheng;Zhong, Jianfeng;Liu, Xianjin;Tu, Sicong
作者机构:
关键词: Domain antibody;Enzyme-linked immunosorbent assay;Phenoxybenzoic acid;Pyrethroid
期刊名称:ANALYTICAL BIOCHEMISTRY ( 影响因子:3.365; 五年影响因子:3.049 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: An antibody to phenoxybenzoic acid (PBA), the conserved chemical region of pyrethroids, was developed using a domain antibody (DAB) library to enable pyrethroid detection in agricultural products. The DAB library, constructed without animal immunization and based on a human VH framework, displayed repertoires on filamentous bacteriophage. After four rounds of panning, we obtained five domain antibodies that are capable of binding to PBA. Antibody A3 has strong identification capability to cypermethrin, beta-cypermethrin, and fenvalerate. The antibody A3 was used to develop an enzyme-linked immunosorbent assay (ELISA). The IC50 values were 2.586, 1.814, and 2.251 mu g/ml for cypermethrin, beta-cypermethrin, and fenvalerate, respectively. The assay shows weak competition with flucythrinate but shows no competition with fenpropathrin, deltamethrin, and permethrin. The developed ELISA process was successfully applied to fortified Chinese cabbage samples, with the recoveries of cypermethrin, cypermethrin, and fenvalerate ranging from 84.4 to 112.3%. We developed an immunoassay to detect pyrethroids depending on the domain antibody library, which overcomes the limitation of requiring protein antigen to immunize animals raising antibody. (C) 2016 Elsevier Inc. All rights reserved.
分类号: Q5`Q`O65
- 相关文献
作者其他论文 更多>>
-
Combined detection of foodborne pathogens in irrigation water in Jiangsu, China
作者:Xie, Yajing;Zhang, Cunzheng;Liu, Xianjin;Xie, Yajing;Lu, Jiayao;Liu, Xianjin;Li, Bin;Fan, Bochao;Li, Dandi;Du, Xuefei;Xie, Yajing;Schmidt, Stefan
关键词:Irrigation water; Foodborne pathogens; Combined detection; Noroviruses; Strawberry production
-
A fast and efficient virtual screening and identification strategy for helix peptide binders based on finDr webserver: A case study of bovine serum albumin (BSA)
作者:Bu, Jiarui;Luo, Na;Liu, Yuan;Luo, Chuping;Zhang, Xiao;Bu, Jiarui;Luo, Na;Shen, Cheng;Xu, Chongxin;Zhu, Qing;Chen, Chengyu;Xie, Yajing;Liu, Xianjin;Liu, Yuan;Zhang, Xiao
关键词:Virtual screening; Affinity identification; Molecular simulations
-
Impact of Hormone on Growth and GA3 Regulation of Anthocyanin Biosynthesis in Suspension-Culture Cells of Cyclocarya paliurus
作者:Pan, Chuanqing;Liu, Yuan;Chen, Jiguang;Yin, Zhongping;Tang, Daobang
关键词:anthocyanin; Cyclocarya paliurus; gibberellin A(3); multi-omics; plant cell culture
-
Analysis of the effect of three different blanching processes on the flavor profile of peeled and unpeeled broad beans
作者:Shi, Lu;Fu, Xiaohui;Lin, Menghua;Li, Yahui;Liang, Ying;Zhang, Zhiyong;Fu, Xiaohui
关键词:Broad bean; Blanching; HS-GC-IMS; Volatile compounds; Antioxidant activity
-
Enhancing the formation of functional glucosinolate degradation products in fermented broccoli stalk by-product with lactic acid bacteria
作者:Wu, Qinghang;Liu, Daqun;Zhang, Jianming;Li, Ting;Niu, Haiyue;Xin, Xiaoting;Zhang, Chengcheng;Wu, Qinghang;Zhao, Shengming;He, Chengyun
关键词:Broccoli stalk by-product; Glucosinolates; Isothiocyanates; Degradation products; Lactic acid bacteria
-
Synergistic use of stay-green traits and UAV multispectral information in improving maize yield estimation with the random forest regression algorithm
作者:Liu, Yuan;Meng, Lin;Nie, Chenwei;Liu, Yadong;Song, Yang;Jin, Xiuliang;Liu, Yuan;Fan, Kaijian;Meng, Lin;Nie, Chenwei;Liu, Yadong;Song, Yang;Jin, Xiuliang;Cheng, Minghan
关键词:UAV multispectral; Maize yield; Stay-Green Index (SGI); Machine learning; Remote sensing
-
Manipulation of glycine-serine and flavanone metabolism to maintain plasma membrane stability and improve drought tolerance of millet
作者:Wang, Mengjiao;Guo, Jixun;Zhang, Tao;Shi, Lianxuan;Wang, Bianyin;Liu, Yajie;Zhang, Wenying;Wang, Bianyin;Liu, Yajie;Zhang, Wenying;Zhang, Xiao
关键词:Drought; Metabolomics; Millet; Roots; Transcriptomics