Isolation of broad-specificity domain antibody from phage library for development of pyrethroid immunoassay

文献类型： 外文期刊

第一作者： Zhao, Yanyan

作者： Zhao, Yanyan;Zhao, Shengming;Tu, Kang;Liang, Ying;Liu, Yuan;Zhang, Xiao;Hu, Xiaodan;Wu, Aihua;Zhang, Cunzheng;Zhong, Jianfeng;Liu, Xianjin;Liang, Ying;Liu, Yuan;Zhang, Xiao;Hu, Xiaodan;Wu, Aihua;Zhang, Cunzheng;Zhong, Jianfeng;Liu, Xianjin;Tu, Sicong

作者机构：

关键词： Domain antibody;Enzyme-linked immunosorbent assay;Phenoxybenzoic acid;Pyrethroid

期刊名称：ANALYTICAL BIOCHEMISTRY （ 影响因子：3.365； 五年影响因子：3.049 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： An antibody to phenoxybenzoic acid (PBA), the conserved chemical region of pyrethroids, was developed using a domain antibody (DAB) library to enable pyrethroid detection in agricultural products. The DAB library, constructed without animal immunization and based on a human VH framework, displayed repertoires on filamentous bacteriophage. After four rounds of panning, we obtained five domain antibodies that are capable of binding to PBA. Antibody A3 has strong identification capability to cypermethrin, beta-cypermethrin, and fenvalerate. The antibody A3 was used to develop an enzyme-linked immunosorbent assay (ELISA). The IC50 values were 2.586, 1.814, and 2.251 mu g/ml for cypermethrin, beta-cypermethrin, and fenvalerate, respectively. The assay shows weak competition with flucythrinate but shows no competition with fenpropathrin, deltamethrin, and permethrin. The developed ELISA process was successfully applied to fortified Chinese cabbage samples, with the recoveries of cypermethrin, cypermethrin, and fenvalerate ranging from 84.4 to 112.3%. We developed an immunoassay to detect pyrethroids depending on the domain antibody library, which overcomes the limitation of requiring protein antigen to immunize animals raising antibody. (C) 2016 Elsevier Inc. All rights reserved.

分类号： Q5`Q`O65

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